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A kind of bursal heptapeptide for promoting vaccine immune response and its application

A technology of bursa polypeptide and bursa, which is applied in the field of bursa active heptapeptide, can solve problems such as economic losses in poultry industry, hidden dangers of public health safety, threats to human health, etc., to promote extensive immune enhancement and simple structure , Improving the effect of vaccine immunity

Active Publication Date: 2019-11-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The H9N2 avian influenza virus can not only infect poultry, but also gradually has the ability to infect mammals including humans. a huge threat

Method used

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  • A kind of bursal heptapeptide for promoting vaccine immune response and its application
  • A kind of bursal heptapeptide for promoting vaccine immune response and its application
  • A kind of bursal heptapeptide for promoting vaccine immune response and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and identification of BP7

[0028] Wash 500 g of AA broiler bursa tissues without fascia and adipose tissue once with 0.85% physiological saline (precooled to 4-10° C.). After draining, place the bursa tissue in a tissue masher, add pre-cooled saline, and homogenize at high speed three times, 30 seconds each time, and keep the homogenization process at 0-10°C. Then, the homogenate was ultrasonically lysed twice at 4°C, 5 min each time. Then the lysate was heated to 80°C, kept warm for 5min, then quickly placed on ice, and cooled to 10°C. The lysate was then refrigerated and centrifuged for 30 minutes (4000×g / min). The supernatant was collected and freeze-thawed twice. Then the supernatant was centrifuged at high speed at 12000g / min, 4°C, 30min. The supernatant was collected for ultrafiltration, and the ultrafiltrate with a molecular weight below 1000 Da was collected, that is, the crude extract of the bursa tissue. After freeze-drying, dilute with ultr...

Embodiment 2

[0030] 1. Synthetic BP7

[0031] A commercial peptide synthesis company is entrusted to synthesize the peptide according to the GGCDGAA sequence (SEQ ID No.1), and the purity is required to be ≥ 97%.

[0032] 2. Vaccines

[0033] AIV inactivated vaccine (purchased from Nanjing Tianbang Biotechnology Co., Ltd.)

[0034] 3. Grouping of experimental animals

[0035] BALB / C mice are randomly divided into five groups, 10 in every group: (I) PBS control group (every immunization 0.2ml PBS); (II) AIV inactivated vaccine immunization group (every immunization inactivated vaccine 0.2ml ); (III~V) inactivated vaccine+BP7 group (BP7 concentrations were 10, 50, 250ug / mL, and the immunization dose was 0.2ml); the mice in the corresponding group were immunized twice by intraperitoneal injection. The immunization interval was two weeks, 200ul / each / every time (Table 1).

[0036] 4. Sample Collection of Immunized Mice

[0037] After the third immunization, blood was collected from the orb...

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Abstract

The invention relates to separation, authentication and application of a bursa fabricius heptapeptide for promoting vaccine immunoreaction. The immunoactive peptide is a small-molecular polypeptide from bursa fabricius, has the amino acid compoiiton of Gly Gly Cys Asp Gly Ala Ala and is simple in structure and extremeley weak in immunogenicity. The active peptide has a function of promoting the vaccine immunoreaction and has effect of simulating antibody generation and cell immunoreaction, enhancing lymphocyte activity and improving vaccine immunity on mice and chickens. The heptapeptide can be applied to the animal vaccine application research as a vaccine adjuvant or immunopotentiator so as to improve the immune response ability of animal bodies for specific antigens and improve the vaccine immunity, thereby improving the disease infection resistant ability of the animal bodies, and can be applied to the fields of basic immune research, clinical application research and the like.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a heptapeptide capable of promoting vaccine immune response and its application. Background technique [0002] Avian Influenza (AI) is an acute, septic infectious disease caused by type A influenza virus, which can infect almost all wild and domestic poultry. H9N2 subtype avian influenza virus (AIV) has been widespread in my country and around the world since the 1990s. The H9N2 avian influenza virus can not only infect poultry, but also gradually has the ability to infect mammals including humans. a huge threat. Of concern is the 2013 outbreak of H7N9 avian influenza in China, which can infect and kill humans. Through the genetic analysis of its various fragments, it was found that its internal genes were highly homologous to H9N2, which also confirmed that the virus had recombined. These incidents, which have been reported in large numbers...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61K39/39A61K39/145A61P37/04A61P31/16
CPCA61K38/00A61K39/00A61K39/12C07K7/08C12N2760/16134
Inventor 冯秀丽宗嫚嫚余远楠郑阳曹瑞兵陈溥言
Owner NANJING AGRICULTURAL UNIVERSITY
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