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Method for preparing phospholipid vesicle by utilizing highly oriented pyrolytic graphite

A pyrolytic graphite, highly directional technology, applied in chemical instruments and methods, chemical/physical/physicochemical processes of energy application, chemical/physical/physicochemical processes, etc., can solve the problems of poor uniformity of phospholipid vesicles, etc., to achieve The effect of fast formation, simple operation and mild reaction conditions

Inactive Publication Date: 2019-01-01
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of poor uniformity of phospholipid vesicles prepared by the existing electroforming method, and propose a method for preparing phospholipid vesicles using highly oriented pyrolytic graphite as an electrode

Method used

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  • Method for preparing phospholipid vesicle by utilizing highly oriented pyrolytic graphite
  • Method for preparing phospholipid vesicle by utilizing highly oriented pyrolytic graphite
  • Method for preparing phospholipid vesicle by utilizing highly oriented pyrolytic graphite

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specific Embodiment approach 1

[0025] Embodiment 1: In this embodiment, the method for preparing phospholipid vesicles using highly oriented pyrolytic graphite as an electrode is carried out according to the following steps:

[0026] 1. Preparation of phospholipid solution

[0027] Dissolving phospholipids in ethanol to obtain a phospholipid solution;

[0028] 2. Preparation of planar highly oriented pyrolytic graphite electrodes loaded with phospholipid dry film

[0029] Uniformly coating the phospholipid solution prepared in step 1 on one surface of a planar highly oriented pyrolytic graphite electrode, and then vacuum-drying to obtain a planar highly oriented pyrolytic graphite electrode loaded with a phospholipid dry film;

[0030] 3. Assembling the reaction pool

[0031] Take two planar highly oriented pyrolytic graphite electrodes loaded with phospholipid dry film, place the two planar highly oriented pyrolytic graphite electrodes loaded with phospholipid dry film in parallel, and place the phosphol...

specific Embodiment approach 2

[0041] Embodiment 2: This embodiment differs from Embodiment 1 in that the phospholipid in step 1 is dioleoyl phosphatidylcholine, 1-palmitoyl-2-oleoyl phosphatidylcholine or phosphatidylcholine. Other steps and parameters are the same as in the first embodiment.

specific Embodiment approach 3

[0042]Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the mass concentration of ethanol in step 1 is 99.95%. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention relates to a method for preparing phospholipid vesicle by utilizing highly oriented pyrolytic graphite, and relates to a preparation method of phospholipid vesicle, which aims at solvingthe problem that the phospholipid vesicle prepared in an electric forming method is poor in homogeneity. The preparation method comprises the following steps: preparing a phospholipid solution, uniformly smearing the phospholipid solution onto the surface of a plane highly oriented pyrolytic graphite electrode, drying to obtain the plane highly oriented pyrolytic graphite electrode loaded with aphospholipid dry membrane, placing two pieces of the plane highly oriented pyrolytic graphite electrodes loaded with the phospholipid dry membrane in parallel, arranging the phospholipi dry membrane in opposite directions, arranging a cylinder body between the plane highly oriented pyrolytic graphite electrodes loaded with the phospholipid dry membrane to obtain a reaction cell, injecting a reaction medium solution, respectively connecting the graphite electrodes with a signal generator, applying an electric field, thus preparing the phospholipid vesicle. The phospholipid vesicle prepared by the method has controllability and homogeneity, and is low in cost, simple in operation, high in forming speed, mild in reaction conditions, and small in environmental pollution. The method is suitablefor preparing the phospholipid vesicle.

Description

technical field [0001] The invention relates to a preparation method of phospholipid vesicles. Background technique [0002] Phospholipid bilayer membrane is the matrix membrane structure of biological membranes, and is the best model for studying the characteristics of biological membranes, such as membrane osmotic pressure, membrane curvature, membrane tension and membrane elasticity, and the relationship between membrane and nanoparticles. interaction etc. At present, the preparation methods of phospholipid vesicles mainly include microfluidic method, extrusion method, changing solvent method, mild hydration method, electroforming method, etc. Among them, conductive glass is the electrode substrate commonly used in electroforming method. In the existing electroforming method, due to the poor surface roughness and flatness of the substrate used, the uniformity of the formed phospholipid vesicles is poor, and the diameter difference of the phospholipid vesicles formed in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J19/08
CPCB01J19/087
Inventor 毕洪梅史国滨
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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