Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of fingerprint detection method of traditional Chinese medicine composition

A detection method and fingerprint technology, which is applied in the field of fingerprint detection of traditional Chinese medicine compositions, can solve the problems of not providing quality detection methods for traditional Chinese medicine preparations, etc.

Active Publication Date: 2021-06-15
张环宇
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent does not provide a quality testing method for Chinese medicine preparations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of fingerprint detection method of traditional Chinese medicine composition
  • A kind of fingerprint detection method of traditional Chinese medicine composition
  • A kind of fingerprint detection method of traditional Chinese medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Raw materials: 10g of Radix Pseudostellariae, 10g of Rehmannia glutinosa, 6g of Lycium barbarum, 6g of Schisandra, 6g of Polygala, 6g of Shichangpu, 10g of Poria;

[0046] Preparation:

[0047] Add 10 times the amount of 60% ethanol to Schisandra chinensis, heat and reflux extraction for 3 times, each time for 2 hours, combine the extracts, concentrate, and dry under reduced pressure; add 8 times the amount of water to Shichangpu, heat and distill for 8 hours to extract the volatile oil, and the obtained volatile oil can be used as a cyclopaste Fine inclusion (inclusion process: oil: β-CD ratio 1:8, 40°C, stirring for 2 hours); Pseudostellariae, Rehmannia glutinosa, Lycium barbarum, Poria cocos, Polygala add 12 times the amount of water, reflux extraction 3 times, each time After 1.5 hours, the obtained medicinal solution was combined with the water solution of Acorus calamus, concentrated to a relative density of 1.15-1.20 (60°C), added ethanol until the alcohol conten...

Embodiment 2

[0123] Preparation of the test solution: Weigh 0.3g of dry paste powder, put it in a 100ml Erlenmeyer flask, add 9ml of acetone, extract 1 time, ultrasonically extract 1h each time, leave it to room temperature, filter (glass syringe+0.45μm organic needle pore filter membrane), the filtrate ① was obtained and stored for later use; respectively, 9ml of acetone was taken, and the filter residue was washed 2-3 times, and filtered (glass syringe + 0.45 μm organic pinhole filter membrane), the filtrate ② was obtained, and the filtrate ① and ② were combined, and were mixed at 50 Recover ethyl acetate under reduced pressure in a water bath at ℃; dissolve the residue with 10ml of 0.1% phosphoric acid-acetonitrile (40-60), filter through a 0.22 μm filter membrane, take the subsequent filtrate as the test sample, store it at 4°C for testing, take it out and place it at room temperature before measurement .

[0124] Preparation of reference substance solution: Weigh 10 mg of schisandrin ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a fingerprint detection method for a traditional Chinese medicine composition. The method comprises the following steps: preparation of a test solution: taking a traditional Chinese medicine composition to prepare a test solution; preparation of a reference solution: taking schisandrin, Prepare reference solution; Chromatographic conditions: take acetonitrile-phosphoric acid aqueous solution as mobile phase, gradient elution, the concentration of phosphoric acid aqueous solution is 0.05%~0.2%; Determination method: draw reference solution and test solution respectively, inject into liquid phase Chromatograph, measurement; wherein, the traditional Chinese medicine composition is made from the following raw materials by weight: 5-20 parts by weight of Taizishen, 5-20 parts by weight of Shuidi, 4-10 parts by weight of Lycium barbarum, 4-10 parts by weight of Schisandra chinensis parts, 4-10 parts by weight of Polygala radix, 4-10 parts by weight of Shichangpu, and 5-20 parts by weight of Poria.

Description

technical field [0001] The invention relates to a fingerprint detection method of a traditional Chinese medicine composition. Background technique [0002] Fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum that can mark the chemical characteristics of some traditional Chinese medicinal materials or preparations after proper treatment and certain analysis methods. Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of traditional Chinese medicine and is mainly used to evaluate the authenticity, goodness and stability of the quality of Chinese medicinal materials and semi-finished products of traditional Chinese medicine preparations. Fingerprints should have fingerprint properties, namely: (1) strong specificity. It means that the formulated fingerprint should be unique to the traditional Chinese medicine and can be distinguishe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 韩赓倪健王俊宏
Owner 张环宇
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com