Marine invertebrate cell culture method
A technology for invertebrate and cell culture, applied in the field of marine invertebrate cell culture, can solve the problems of late start, lack of stable and reliable cell culture technology solutions for marine invertebrates, etc., and achieve the effect of improving survival rate
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Embodiment 1
[0036] A method for culturing sea cucumber intestinal cells in this embodiment comprises the following steps:
[0037] 1. Culture medium preparation
[0038] (1) primary culture medium
[0039]
[0040] After the preparation is completed, the 0.22 μm filter membrane is filtered to sterilize and refrigerated for later use.
[0041] (2) Secondary medium
[0042]
[0043] After the preparation is completed, the 0.22 μm filter membrane is filtered to sterilize and refrigerated for later use.
[0044] (3) Tertiary medium
[0045]
[0046]
[0047] After the preparation is completed, the 0.22 μm filter membrane is filtered to sterilize and refrigerated for later use.
[0048] 2. Processing of sea cucumber tissue samples
[0049] Dissect the adult sea cucumber (80-100g) under normal growth state, take out the sea cucumber intestine, remove the contents, soak in 75% ethanol for 5 seconds, take it out quickly and wash it in PBS solution or primary medium At least twic...
Embodiment 2
[0058] In this embodiment, a method for culturing embryonic cells of squid without needles comprises the following steps:
[0059] 1. Culture medium preparation
[0060] (1) primary culture medium
[0061]
[0062] After the preparation is completed, the 0.22 μm filter membrane is filtered to sterilize and refrigerated for later use.
[0063] (2) Secondary medium
[0064]
[0065]After the preparation is completed, the 0.22 μm filter membrane is filtered to sterilize and refrigerated for later use.
[0066] (3) Tertiary medium
[0067]
[0068]
[0069] After the preparation is completed, the 0.22 μm filter membrane is filtered to sterilize and refrigerated for later use.
[0070] 2. Handling of Squid Tissue Samples
[0071] Collect squid squid embryos that develop about 15 days after spawning and fertilization, soak them in 75% ethanol for 5 seconds, take them out quickly and wash them at least twice in PBS solution or primary medium to remove ethanol, and th...
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