A synthetic method of wheat in winter wheat area with high genetic diversity and high embryo and seedling rate

A technology of genetic diversity and synthesis method, which is applied in the field of synthesis of wheat, winter wheat with high genetic diversity and high embryo and seedling rate, can solve the problem of limited genetic information, inapplicability of winter wheat area, and Low embryo seedling rate and other problems, to achieve the effect of broadening the genetic basis, wide selection range, and simple operation process

Active Publication Date: 2020-10-16
CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems that existing synthetic wheat is not applicable to winter wheat regions, genetic information is limited, and the rate of embryo and seedling formation is low, the present invention provides a synthesis of wheat from winter wheat regions with high genetic diversity and high embryo and seedling rate. method

Method used

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  • A synthetic method of wheat in winter wheat area with high genetic diversity and high embryo and seedling rate
  • A synthetic method of wheat in winter wheat area with high genetic diversity and high embryo and seedling rate
  • A synthetic method of wheat in winter wheat area with high genetic diversity and high embryo and seedling rate

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Embodiment 1

[0033] A method for synthesizing wheat with high genetic diversity and high embryo and seedling rate, using the following steps:

[0034] A, select durum wheat and rough goat grass to sow by mass and number ratio 2:1, durum wheat is sowed in three phases, every 3 days at intervals, the first stage sowing of rough goat grass;

[0035] B, select durum wheat as the female parent, choose A. chinensis as the male parent, carry out emasculation and bagging to the durum wheat of the mother, and pollinate and bag the 48 hours after emasculation;

[0036] After c, pollination 24 hours, cut off 30cm below the ear of durum wheat plant, and after 30s with tap water flushing, insert in liquid culture medium I and cultivate, culture temperature is 21 ℃, and culture humidity is 80%, and light is 1500lux, 8 hours of light per day, after 14 days of cultivation, the wheat grains are stripped from the ears for sterilization;

[0037] d. Peel off the sterilized immature embryos of wheat grains o...

Embodiment 2

[0045] A method for synthesizing wheat with high genetic diversity and high embryo and seedling rate, using the following steps:

[0046] A, select durum wheat and rough goat grass to sow by mass and number ratio 2:1, durum wheat is sowed in three phases, every 5 days at intervals, the first stage sowing of rough goat grass;

[0047] B, select durum wheat as the female parent, choose A. chinensis as the male parent, carry out emasculation and bagging to the maternal durum wheat, 72 hours after emasculation, pollinate and bag with the newly collected A. chinensis pollen;

[0048] After c, pollination 24 hours, 40cm below the durum wheat plant ear is cut off, and after 30s with tap water flushing cut-off place, insert in liquid culture medium I and cultivate, culture temperature is 25 ℃, and culture humidity is 80%, and light is 2500lux, 8 hours of light per day, after 14 days of cultivation, the wheat grains are stripped from the ears for sterilization;

[0049] d. Peel off th...

Embodiment 3

[0057] A method for synthesizing wheat with high genetic diversity and high embryo and seedling rate, using the following steps:

[0058] A, select durum wheat and rough goat grass to sow by mass and number ratio 2:1, and durum wheat is sowed in three phases, every 4 days at intervals, the first stage sowing of rough goat grass;

[0059] B, select durum wheat as the female parent, choose A. chinensis as the male parent, carry out emasculation and bagging to the durum wheat of the mother, 51 hours after emasculation, pollinate and bag with the pollen of the newly collected A. chinensis pollen;

[0060] After c, pollination 24 hours, cut off 37cm below the ear of durum wheat plant, and after 30s with tap water flushing cut-off place, insert in liquid culture medium I and cultivate, culture temperature is 22 ℃, and culture humidity is 80%, and light is 1900lux, 8 hours of light per day, after 14 days of cultivation, the wheat grains are stripped from the ears for sterilization; ...

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Abstract

The invention specifically relates to a winter wheat region wheat synthesizing method with high genetic diversity and a high embryo formation and seedling formation rate and solves the problems that existing synthesized wheat is not suitable for winter wheat regions, genetic information is limited, and the embryo formation and seedling formation rate is low. The winter wheat region wheat synthesizing method comprises the steps: a, choosing durum wheat and aegilops tauschii to be sowed; b, pollinating pollen of the aegilops tauschii to the durum wheat; c, performing cultivation and sterilization; d, inoculating young embryo of sterilized kernels to a culture medium II to be cultured; e, performing vernalization culture when seedlings are achieved; f, transplanting the seedlings to a land for growing field crops; g, performing soaking treatment through a colchicine solution with the concentration as 1% when the seedlings grow out tillers and then continuing performing field culture and management until the wheat is harvested. The synthesizing method disclosed by the invention is suitable for synthesizing and breeding a wheat novel material of the winter wheat region of northern China.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a synthesis method of wheat, in particular to a synthesis method of wheat in winter wheat regions with high genetic diversity and high embryo and seedling formation rate. Background technique [0002] Common wheat (Triticum aestivum L, AABBDD, 2n=6x=42) is one of the main food crops in the world. During the evolution of common wheat, only a very small number of cultivated emmer wheat and goat grass had naturally crossed to form hexaploid wheat, resulting in a significant decline in the genetic diversity of hexaploid wheat compared with its parents, resulting in the "evolutionary bottleneck". [0003] Since the 1970s, due to the breeding and large-scale promotion of semi-dwarf high-yield wheat varieties, they have replaced the local varieties of wheat with rich variations and good adaptability. This long-term artificial directional selective breeding has accelerate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001A01H4/008
Inventor 闫贵云左静静霍利光任永康左敏牛瑜琦
Owner CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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