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A preparation method of injection for treating androgenetic alopecia using dermal papilla cell exosomes

A technology of dermal papilla cells and androgen source is applied in the field of preparation of injections, which can solve the problems of difficulty in reaching the required amount of treatment, difficulties in exosome research and clinical application, and large differences in the types of main substances, and achieves convenient treatment, Good effect, the effect of a large amount of exosomes

Active Publication Date: 2021-07-16
浙江卫未生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at this stage, the collection of exosomes from stem cells is difficult to meet the therapeutic requirements. At the same time, the amount of secreted exosomes and the types of main substances contained in exosomes are quite different under different environments, different pH and different induction conditions. Both bring troubles to the research and clinical application of exosomes

Method used

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  • A preparation method of injection for treating androgenetic alopecia using dermal papilla cell exosomes
  • A preparation method of injection for treating androgenetic alopecia using dermal papilla cell exosomes
  • A preparation method of injection for treating androgenetic alopecia using dermal papilla cell exosomes

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Embodiment 1

[0028] A method for preparing an injection using dermal papilla cell exosomes to treat androgenetic alopecia, the injection being a physiological saline solution containing dermal papilla cell exosomes obtained in a serum-free basal medium, Its preparation method comprises the following steps:

[0029] (1) Isolation of dermal papilla cells:

[0030] a. A single hair follicle after surgery is stored in the hair follicle collection solution at 4°C, and the hair follicle tissue is washed 2 to 3 times with a double-antibody PBS solution containing 1% mass fraction;

[0031] b. Under the microscope, use a 1ml syringe needle to separate the entire hair bulb tissue, remove the residual hair matrix, and use a pipette gun to move the tissue to a new Petri dish containing PBS solution;

[0032] c. Wash PBS once, add 0.2% collagenase IV, digest at 37°C for 1-2 hours, observe the digestion status at intervals of 10 minutes, and blow the tissue with a straw until the dermal sheath tissue ...

Embodiment 2

[0038] An injection for treating androgenetic alopecia by using dermal papilla cell exosomes, the injection being a physiological saline solution containing dermal papilla cell exosomes.

[0039] The preparation method of the above injection for treating androgenetic alopecia using exosomes from dermal papilla cells comprises the following steps:

[0040] (1) Isolation of dermal papilla cells:

[0041] a. A single hair follicle after surgery is stored in the hair follicle collection solution at 4°C, and the hair follicle tissue is washed 2 to 3 times with a double-antibody PBS solution containing 1% mass fraction;

[0042] b. Under the microscope, use a 1ml syringe needle to separate the entire hair bulb tissue, remove the residual hair matrix, and use a pipette gun to move the tissue to a new Petri dish containing PBS solution;

[0043] c. Wash PBS once, add 0.2% collagenase IV, digest at 37°C for 1-2 hours, observe the digestion status at intervals of 10 minutes, and blow t...

Embodiment 3

[0049] An injection for treating androgenetic alopecia by using dermal papilla cell exosomes, the injection being a physiological saline solution containing dermal papilla cell exosomes.

[0050] The preparation method of the above injection for treating androgenetic alopecia using exosomes from dermal papilla cells comprises the following steps:

[0051] (1) Isolation of dermal papilla cells:

[0052] a. A single hair follicle after surgery is stored in the hair follicle collection solution at 4°C, and the hair follicle tissue is washed 2 to 3 times with a double-antibody PBS solution containing 1% mass fraction;

[0053] b. Under the microscope, use a 1ml syringe needle to separate the entire hair bulb tissue, remove the residual hair matrix, and use a pipette gun to move the tissue to a new Petri dish containing PBS solution;

[0054] c. Wash PBS once, add 0.2% collagenase IV, digest at 37°C for 1-2 hours, observe the digestion status at intervals of 10 minutes, and blow t...

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Abstract

The invention relates to an injection for treating androgen-induced alopecia by using exosomes of dermal papilla cells. The injection is a physiological saline solution containing exosomes of dermal papilla cells. There are five steps: adherent culture of papilla cells, stimulation-induced culture of dermal papilla cells, preparation of exosome freeze-dried powder, and preparation of injection solution. The exosomes secreted by the dermal papilla cells obtained by the preparation method of the present invention have a large amount of exosomes and a high content of effective ingredients for promoting hair regeneration, so that the injection has a good effect in treating androgenetic alopecia.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing an injection for treating androgenetic alopecia by using dermal papilla cell exosomes. Background technique [0002] The prevalence of androgenetic alopecia (AGA) increases with age and affects both men and women. Patients diagnosed with AGA may experience a reduced quality of life. Although natural and synthetic ingredients can be used to treat AGA and promote hair growth, they have serious side effects and unknown mechanisms of action that limit their use. At present, there are studies using the hair follicles of patients' own normal hair to isolate dermal papilla cells (DPC), which play a key role in hair follicle regeneration, to obtain exosomes by culturing and proliferating them in vitro, and injecting them into the patient's bald area to generate new normal hair follicles. hair. The researchers further found that during the process of exosomes promoti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/36A61K9/08A61K47/02A61P17/14C12N5/071
CPCA61K9/0019A61K35/36A61K47/02A61P17/14C12N5/0627C12N2509/00
Inventor 袁博陈锦阳刘军权
Owner 浙江卫未生物医药科技有限公司
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