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Injection capable of treating androgenetic alopecia with dermal papilla cell exosomes

A dermal papilla cell and androgen source technology, applied in the field of injection for the treatment of androgen-induced alopecia and its preparation, can solve the problem of difficult to achieve the required amount of treatment, problems of exosome research and clinical application, and relatively different types of main substances. Large and other problems, to achieve the effect of convenient treatment, good effect, and large amount of exosomes

Active Publication Date: 2018-12-14
浙江卫未生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, at this stage, the collection of exosomes from stem cells is difficult to meet the therapeutic requirements. At the same time, the amount of secreted exosomes and the types of main substances contained in exosomes are quite different under different environments, different pH and different induction conditions. Both bring troubles to the research and clinical application of exosomes

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  • Injection capable of treating androgenetic alopecia with dermal papilla cell exosomes
  • Injection capable of treating androgenetic alopecia with dermal papilla cell exosomes
  • Injection capable of treating androgenetic alopecia with dermal papilla cell exosomes

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Embodiment 1

[0029] An injection for treating androgenetic alopecia by using dermal papilla cell exosomes, the injection being a physiological saline solution containing dermal papilla cell exosomes.

[0030] The preparation method of the above injection for treating androgenetic alopecia using exosomes from dermal papilla cells comprises the following steps:

[0031] (1) Separation of dermal papilla cells:

[0032] a. A single hair follicle after surgery is placed in the hair follicle collection solution and stored at 4°C, and the hair follicle tissue is washed 2 to 3 times with a double-antibody PBS solution containing 1% mass fraction;

[0033] b. Under the microscope, use a 1ml syringe needle to separate the entire hair bulb tissue, remove the remaining hair matrix, and use a pipette gun to move the tissue to a new Petri dish containing PBS solution;

[0034] c. Wash PBS once, add 0.2% collagenase IV, digest at 37°C for 1-2 hours, observe the digestion status at intervals of 10 minute...

Embodiment 2

[0040] An injection for treating androgenetic alopecia by using dermal papilla cell exosomes, the injection being a physiological saline solution containing dermal papilla cell exosomes.

[0041] The preparation method of the above injection for treating androgenetic alopecia using exosomes from dermal papilla cells comprises the following steps:

[0042] (1) Separation of dermal papilla cells:

[0043] a. A single hair follicle after surgery is placed in the hair follicle collection solution and stored at 4°C, and the hair follicle tissue is washed 2 to 3 times with a double-antibody PBS solution containing 1% mass fraction;

[0044] b. Under the microscope, use a 1ml syringe needle to separate the entire hair bulb tissue, remove the remaining hair matrix, and use a pipette gun to move the tissue to a new Petri dish containing PBS solution;

[0045]c. Wash PBS once, add 0.2% collagenase IV, digest at 37°C for 1-2 hours, observe the digestion status at intervals of 10 minutes...

Embodiment 3

[0051] An injection for treating androgenetic alopecia by using dermal papilla cell exosomes, the injection being a physiological saline solution containing dermal papilla cell exosomes.

[0052] The preparation method of the above injection for treating androgenetic alopecia using exosomes from dermal papilla cells comprises the following steps:

[0053] (1) Separation of dermal papilla cells:

[0054] a. A single hair follicle after surgery is placed in the hair follicle collection solution and stored at 4°C, and the hair follicle tissue is washed 2 to 3 times with a double-antibody PBS solution containing 1% mass fraction;

[0055] b. Under the microscope, use a 1ml syringe needle to separate the entire hair bulb tissue, remove the remaining hair matrix, and use a pipette gun to move the tissue to a new Petri dish containing PBS solution;

[0056] c. Wash PBS once, add 0.2% collagenase IV, digest at 37°C for 1-2 hours, observe the digestion status at intervals of 10 minute...

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Abstract

The invention relates to an injection capable of treating androgenetic alopecia with dermal papilla cell exosomes. The injection is a normal saline solvent containing dermal papilla cell exosomes. A preparation method of the injection comprises fiver steps including dermal papilla cell separation, dermal papilla cell adherent culture, dermal papilla cell stimulation induced culture, exosome freezedried powder preparation, and injection preparation. The amount of the exosomes secreted by the dermal papilla cells prepared using the preparation method is large, the content of effective components capable of promoting hair regeneration is high, and the treatment effect of the injection on androgenetic alopecia is excellent.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an injection for treating androgenetic alopecia by using dermal papilla cell exosomes and a preparation method thereof. Background technique [0002] The prevalence of androgenetic alopecia (AGA) increases with age and affects both sexes. Patients diagnosed with AGA may experience a reduced quality of life. Although natural and synthetic ingredients can be used to treat AGA and promote hair growth, they have serious side effects and unknown mechanisms of action that limit their use. At present, there are studies using the hair follicles of the patient's own normal hair to isolate dermal papilla cells (DPC), which play a key role in hair follicle regeneration, to culture and proliferate them in vitro to obtain exosomes, and inject them into the patient's bald area to generate new normal hair follicles. hair. The researchers further found that during the process of exosomes promotin...

Claims

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Application Information

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IPC IPC(8): A61K35/36A61K9/08A61K47/02A61P17/14C12N5/071
CPCA61K9/0019A61K35/36A61K47/02A61P17/14C12N5/0627C12N2509/00
Inventor 袁博陈锦阳刘军权
Owner 浙江卫未生物医药科技有限公司
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