A closed bioreactor and cell culture method thereof

Abstract

The invention discloses a closed bioreactor, comprising a solution container, a collection container, a biological reaction tank, a liquid inlet pipe and a liquid outlet pipe, and the liquid inlet pipe and the liquid outlet pipe are each provided with a cross. The invention also discloses a cell culture method for a closed bioreactor, including pre-cultivation; bioreactor cell inoculation; bioreactor cell perfusion culture; bioreactor virus inoculation; bioreactor washing; bioreactor virus perfusion culture . The invention can realize the perfusion culture of the bioreactor, realize the rapid liquid change, facilitate cleaning and high pressure, can be reused many times, save the production cost, and can also reduce the pollution risk and improve the work efficiency. The device and the cell culture method thereof replace the existing method for single-pipe bioreactor production operation, which not only effectively ensures the sterility requirements in vaccine production, but also is easy to operate and suitable for large-scale cultivation of fixed-bed bioreactors.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a closed bioreactor and a cell culture method thereof. Background technique [0002] In the production of biological products, bioreactors are often required for large-scale cell culture. Traditional bioreactor cultures are mostly fed-batch, but in recent years there has been a growing interest in continuous processes. Compared with batch or fed-batch culture, perfusion culture can give cells better nutrients and environment for growth, so that cells can reach a better state, and finally can achieve higher cell densities and correspondingly higher unit protein production. Rate. The advantage of this is that the perfusion culture can obtain an order of magnitude more target products. [0003] The control of sterility in the vaccine production process is very important, which will directly affect the quality of the product. Strict aseptic operation is required in ever...

AI Technical Summary

Problems solved by technology

Bioreactor perfusion culture is generally a single-function inlet and outlet pipe. In the actual production process, it needs to be disassembled and assembled many times. The workload is heavy and it is easy to be confused, which increases the risk of contamination.

In short, this kind of pipeline is not only cumbersome, time-consuming and labor-intensive in the operation process, but also has a high risk of contamination during the operation process

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