Enclosed bioreactor and cell culturing method thereof

Abstract

The invention discloses an enclosed bioreactor. The enclosed bioreactor comprises a solution container, a collection container, a bioreaction tank, a liquid inlet pipeline and a liquid outlet pipeline, wherein the liquid inlet pipeline and the liquid outlet pipeline are provided with one four-way joint respectively. The invention further discloses a cell culturing method of the enclosed bioreactor. The cell culturing method comprises pre-culturing, bioreactor cell inoculation, bioreactor cell perfusion culture, bioreactor virus vaccination, bioreactor washing and replacement as well as bioreactor virus perfusion culture. By adopting the enclosed bioreactor, bioreactor perfusion culture can be realized, rapid liquid exchanging is realized, high-pressure cleaning is facilitated, repeated used is realized, the production cost is reduced, the pollution risk can be lowered, and the working efficiency is improved. By adopting the enclosed bioreactor and the cell culturing method thereof disclosed by the invention, the production operation of the bioreactor with a single pipeline in the existing method is substituted, and the aseptic requirement in vaccine production is effectively ensured. The enclosed bioreactor is easy and convenient to operate, and is suitable for fixed bed bioreactor large-scale culture.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a closed bioreactor and a cell culture method thereof. Background technique [0002] In the production of biological products, large-scale cell culture usually requires the use of bioreactors. Traditional bioreactor cultures are mostly fed-batch cultures, but in recent years, there has been increasing interest in continuous processes. Compared with batch or fed-batch culture, perfusion culture can provide better nutrition and environment for cell growth, so that cells can achieve a better state, and finally achieve higher cell density and correspondingly higher unit protein production. Rate. The advantage of this is that the perfusion culture can obtain an order of magnitude more target products. [0003] Aseptic control is very important in the vaccine production process, which will directly affect the quality of the product. Strict aseptic operation is required in...

AI Technical Summary

Problems solved by technology

Bioreactor perfusion culture is generally a single-function inlet and outlet pipe. In the actual production process, it needs to be disassembled and assembled many times. The workload is heavy and it is easy to be confused, which increases the risk of contamination.

In short, this kind of pipeline is not only cumbersome, time-consuming and labor-intensive in the operation process, but also has a high risk of contamination during the operation process

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