Method for extracting livetin and oil from larimichthys crocea eggs at the same time

Abstract

The invention discloses a method for extracting livetin and oil from larimichthys crocea eggs at the same time. The method comprises the steps of larimichthys crocea egg freeze-dried powder preparation, freeze-dried powder dissolution, primary extraction, precipitation dissolution, secondary extraction, mixing and dialysis. According to the method for extracting the livetin and the oil from the larimichthys crocea eggs at the same time, a sodium chloride solution is adopted for digestion at a room temperature, and through centrifugation, the livetin and the oil are separated according to different tensity so that the oil and protein can be recovered at the same time; the livetin recovery rate is up to 76-82%, a kjeldah method is used for detecting the protein purity which is up to 80% or above, and through mass spectrum identification, the livetin content accounts for more than 99% of the total protein content; the oil recovery rate reaches 22-23%, and the oil purity is reaches 90% orabove; the emulsibility of the larimichthys crocea egg livetin prepared through the method is significantly improved compared with that of the freeze-dried powder and is significantly better than thatof soy isolate protein, so that the method has important significance in larimichthys crocea egg deep-processing.

Description

technical field [0001] The invention relates to the technical field of aquatic products processing, and relates to a preparation method of large yellow croaker egg yolk protein and oil, more specifically, relates to a method for simultaneously extracting large yellow croaker egg yolk protein and oil. Background technique [0002] Large yellow croaker roe is the main by-product of large yellow croaker processing, accounting for about 15% to 25% of the total mass of the fish. Its protein content is as high as 59.03±0.08% (by dry weight), and it is rich in oil, so , how to effectively develop and utilize large yellow croaker egg yolk protein and oil has gained more and more people's attention. At present, many large yellow croaker roe in my country are discarded except for being made into feed, which causes a great waste of resources and environmental pollution. [0003] Regarding the literature reports on large yellow croaker roe, most scholars focus on unilateral utilization...

AI Technical Summary

Problems solved by technology

At present, many large yellow croaker eggs in my country are discarded except for being made into feed, which causes a great waste of resources and environmental pollution.

[0003] Regarding the literature reports of large yellow croaker roe, most scholars focus on unilateral utilization, such as the research on the functional properties of large yellow croaker roe muscle layer protein or the research on the oil of large yellow croaker roe. Part of it is discarded, which causes a waste of resources

[0004] The relative content of protein in large yellow croaker roe is as high as 59.03%, and the oil content is also very rich. However, there is no report on the method of obtaining protein and oil from large yellow croaker roe at the same time, and the problem of simultaneously preparing large yellow croaker egg yolk protein and oil has not been solved.

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