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Preparation method of ZnO/GQD-PEI composite quantum dot antibacterial agent

An antibacterial agent and quantum dot technology, which is applied in the field of preparation of ZnO/GQD-PEI composite quantum dot antibacterial agent, can solve the problems of large structural difference, large size, and small number of adsorption active sites, and achieves strong drug resistance, Good biocompatibility and high antibacterial activity

Active Publication Date: 2018-12-11
四川华运天晨新材料技术有限公司
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Problems solved by technology

But it is worth noting that the ZnO / GO composites prepared in the above studies are relatively large in size, usually 100-1000nm, and it is difficult to enter the interior of bacterial cells and kill bacteria
In addition, the composition and structure of nanomaterials and bacterial cell membranes are quite different, resulting in a relatively small number of adsorption active sites that interact with bacteria.

Method used

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  • Preparation method of ZnO/GQD-PEI composite quantum dot antibacterial agent
  • Preparation method of ZnO/GQD-PEI composite quantum dot antibacterial agent
  • Preparation method of ZnO/GQD-PEI composite quantum dot antibacterial agent

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preparation example Construction

[0025] A preparation method of ZnO / GQD-PEI composite quantum dot antibacterial agent, comprising the following steps:

[0026] Step 1: Preparation of graphene quantum dots (GQDs) solution:

[0027] Put 1-10g of citric acid (CA) into a beaker and heat to obtain an orange liquid; then, add sodium hydroxide solution dropwise to adjust the pH=7.0, dialyze and rotary evaporate to obtain a graphene quantum dot (GQDs) solution ;

[0028] Step 2: Preparation of GQD-PEI:

[0029] Dilute the GQDs obtained in step 1 to 0.2-2mg / ml with deionized water, add a modifier to make the concentration 5-15mg / ml, and after ultrasonic treatment for 10min, add 0.05-0.2g 1-ethyl-(3- Dimethylaminopropyl) carbodiimide hydrochloride (EDC•HCl), ultrasonic treatment for 20min, then add 0.2-0.5g EDC•HCl, ultrasonic treatment for 20min, magnetic stirring at room temperature for 24h, to obtain GQD-PEI;

[0030] Step 3: Preparation of modified ZnO / GQD-PEI composite quantum dot antibacterial agent:

[0031]...

Embodiment 1

[0039] Step 1: Put 2g of citric acid (CA) into a 50ml beaker, heat to 200°C and react for 30 minutes to obtain an orange liquid; then, adjust the pH to 7.0 with aqueous sodium hydroxide solution, dialyze for 24 hours, and rotate to evaporate to obtain the GQDs solution;

[0040] Step 2: Dilute the GQDs obtained in step 1 to 0.5 mg / ml with deionized water, add polyethyleneimine (PEI) to make the concentration 8 mg / ml, and after ultrasonic treatment for 10 min, add 0.1 g 1-ethyl-( 3-Dimethylaminopropyl) carbodiimide hydrochloride (EDC•HCl), ultrasonic treatment for 20min, then add 0.3g EDC•HCl, ultrasonic treatment for 20min, magnetic stirring at room temperature for 24h, to obtain GQD-PEI ;

[0041] Step 3: Add 0.3 g of potassium hydroxide to 50 ml of ethanol solution, and stir magnetically for 30 minutes to obtain solution A; add 0.587 g of zinc acetate dihydrate to 100 ml of ethanol solution, and stir magnetically for 30 minutes to obtain solution B; Add the GQD-PEI obtained...

Embodiment 2

[0043] Step 1: Put 4g of citric acid (CA) into a 50ml beaker, heat to 200°C and react for 30 minutes to obtain an orange liquid; then, adjust the pH to 7.0 with aqueous sodium hydroxide solution, dialyze for 24 hours, and rotate to evaporate to obtain the GQDs solution;

[0044] Step 2: Dilute the GQDs obtained in Step 1 to 1 mg / ml with deionized water, add polyethyleneimine (PEI) to make the concentration 10 mg / ml, and after ultrasonic treatment for 10 min, add 0.12 g of 1-ethyl-(3 - Dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), after ultrasonic treatment for 20 minutes, then add 0.388g EDC·HCl, ultrasonic treatment for 20 minutes, magnetic stirring at room temperature for 24 hours, to obtain GQD-PEI;

[0045] Step 3: Add 0.42g of sodium hydroxide to 50ml of ethanol solution, and stir magnetically for 30min to obtain solution A; add 0.78g of zinc acetate dihydrate to 100ml of ethanol solution, and stir magnetically for 30min to obtain solution B; Add the GQD-PEI ob...

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Abstract

The invention relates to a preparation method of a ZnO / GQD-PEI composite quantum dot antibacterial agent. The preparation method comprises the following steps: heating citric acid to obtain an orangesolution; dropwise adding a NaOH solution to adjust pH, and performing dialysis and rotary evaporation, thus obtaining a graphene quantum dot (GQDs) solution; firstly diluting the obtained GQDs solution, then adding modifier to adjust the concentration, performing the ultrasonic treatment, adding EDC-HCl in two times, ultrasonically stirring, and obtaining GQD-PEI; separately adding an alkaline source and a zinc source into an ethanol solution, stirring, and obtaining a solution A and a solution B; adding the obtained GQD-PEI into the solution B, ultrasonically dispersing, then adding the solution A into the solution B, heating in a water bath, reacting, then washing, and centrifuging, thus obtaining the composite quantum dot antibacterial agent. Compared with the traditional antibacterialagent, the nano composite antibacterial agent prepared in the invention has the advantages of safety, environmental protection, high drug resistance and the like, and the minimum antibacterial concentration for escherichia coli can reach 1.8 mg / ml.

Description

technical field [0001] The invention relates to the research of a novel high-efficiency antibacterial agent, in particular to a preparation method of a ZnO / GQD-PEI composite quantum dot antibacterial agent. Background technique [0002] In recent years, due to the irrational exploitation of resources, the resources are scarce and the natural environment has been damaged, leading to diseases caused by various pathogenic bacteria, fungi and viruses that threaten human health. The research and development of various antibacterial agents has become a current research hotspot, among which inorganic antibacterial materials are favored because of their good safety, durability, chemical stability, and low cost. [0003] At present, the inorganic antibacterial agents that have been studied more are silver-based antibacterial agents, but silver-based antibacterial agents are easy to change color, Ag + Dissolution is unfavorable to the human body, which greatly limits its application...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/44A61K47/59A61P31/04A61K33/30
CPCA61K33/30A61K33/44A61K47/59A61P31/04B82Y5/00B82Y40/00Y02A50/30B82Y30/00
Inventor 刘俊莉邵建真马建中刘辉李军奇
Owner 四川华运天晨新材料技术有限公司
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