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Detection reagent card for typing test of helicobacter pylori and preparation method of detection reagent card

A technology of Helicobacter pylori and detection reagent, applied in the field of quantum dot immunofluorescence chromatography in medical immunology

Active Publication Date: 2018-12-07
重庆新赛亚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many detection methods, there are very few that are simple, fast and able to detect typing

Method used

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  • Detection reagent card for typing test of helicobacter pylori and preparation method of detection reagent card
  • Detection reagent card for typing test of helicobacter pylori and preparation method of detection reagent card
  • Detection reagent card for typing test of helicobacter pylori and preparation method of detection reagent card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Preparation of detection reagent card

[0040] 1. Optimization of sample pad 1:

[0041] 1.1. Materials and reagents:

[0042] (1) Sample pad 1: glass fiber material.

[0043] (2) Sample pad optimization buffer: 50 mmol / L, pH8.2 tris (tris), 0.5 wt% Tween 20, 0.1 wt% proclin300, and remaining purified water.

[0044] 1.2. Immerse the sample pad 1 in the sample pad optimization buffer for 30-45 minutes, and then dry it for more than 5 hours in the drying room, and control the humidity below 30%.

[0045] 2. Optimization of quantum pad 2:

[0046] 2.1. Materials and reagents

[0047] (1) Quantum pad 2: glass fiber material.

[0048] (2) Quantum pad optimization buffer: 50mmol / L, pH8.2 tris (tris), 1% mass percent bovine serum albumin (BSA), 0.5 mass percent concentration Tween 20, 2% sucrose by mass, 0.1% proclin300 by mass, and the remainder of purified water.

[0049] 2.2. Immerse the quantum pad 2 in the quantum pad optimization buffer for 30-45 minu...

Embodiment 2

[0098] Embodiment 2, Helicobacter pylori typing detection method

[0099] (1) The serum to be tested was respectively added to the sample pad (1) of the detection reagent card of Example 1, and the reaction was carried out at room temperature for 15 min.

[0100] (2) Insert the detection reagent card into the iSIA01 fluorescence immunoassay analyzer, and read the negative and positive of the antibody.

[0101] Test methods and test results

[0102] (1) A large number of three types of normal negative serum were used to measure the concentration value. Since the reliability was greater than 99%, the mean value + 3SD of each serum was determined as the positive judgment value.

[0103] (2) The minimum detection limit of the detection reagent card of Example 1 of the present invention: the sensitivity reference products of Helicobacter pylori urease Urease antibody, cytotoxin-related protein CagA antibody and vacuolar cytotoxin VacA antibody are respectively 1:3, 1:3: 9. For 1:...

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PUM

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Abstract

The invention relates to a detection reagent card for typing test of helicobacter pylori. The detection reagent card comprises a sample pad, a quantum pad, a nitrocellulose membrane, absorbent paper and a lining plate, wherein a quantum dot microsphere labeled antigen complex is coated on the quantum pad; the antigen complex comprises a Urease antigen, a CagA antigen, a VacA antigen and rate IgG;a first detection line, a second detection line, a third detection line and a quality control line are arranged on the nitrocellulose membrane; the VacA antigen is coated on the first detection line;the CagA antigen is coated on the second detection line; the Urease antigen is coated on the third detection line; and the goat anti-rat IgG is coated on the quality control line. The detection reagent card for typing test of helicobacter pylori provided by the invention is capable of rapidly and accurately obtaining the results, the infection type of the helicobacter pylori can be judged according to the detection result, and auxiliary diagnosis is further provided for clinical related diseases.

Description

technical field [0001] The invention belongs to the technical field of quantum dot immunofluorescence chromatography in medical immunology, and particularly relates to a detection reagent card for Helicobacter pylori typing detection and a preparation method thereof. Background technique [0002] Epidemiological studies have shown that the infection rate of Helicobacter pylori (HP) is very high, and about 50% of the world's people carry HP, which has been recognized by the International Cancer Organization as the most important cause of gastric ulcers, chronic gastritis and gastric cancer. causative factor. The incidence rate is closely related to socioeconomic level, population density, public health conditions and water supply. Epidemiological studies have shown that HP infection mostly occurs in childhood, and in the absence of antibiotic treatment, HP infection usually accompanies life-long; In addition, HP infection is also the most widespread chronic bacterial infect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56916
Inventor 张念林赵亚丽孙婵马丹丹左云国
Owner 重庆新赛亚生物科技有限公司
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