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Brucellosis detection card and preparation method thereof

A technology for brucellosis and brucellosis, which is applied in the direction of analyzing materials through chemical reactions and material analysis through observation of the influence of chemical indicators, and can solve the problem that the judgment results are greatly affected by subjective factors , inconvenient storage of test results, long time-consuming test and other problems, to achieve the effect of shortening the test cycle, simple structure and easy operation

Inactive Publication Date: 2018-12-07
石家庄久正生物科技有限公司
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Problems solved by technology

Although the test tube agglutination test has always been used as an important method for serological detection of brucellosis, it has disadvantages such as cumbersome operation, long test time, need for heating in an incubator, subjective factors to determine the results, and inconvenient storage of test results.

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  • Brucellosis detection card and preparation method thereof

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Embodiment Construction

[0028] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0029] A kind of brucellosis detection card, described detection card comprises 2-10 microtubes, contains antigen solution and glass microbead in the microtube, and described antigen solution is made of brucella bacterial body fluid, carbolic acid, BSA, phenolic acid Sodium protein, eosin and saline.

[0030] The preferred scheme is that the formula of the antigen solution is: Brucella bacterial body fluid 0.01-0.08wt%, carbolic acid 0.5-1wt%, BSA 0.6-1.2wt%, casein sodium 0.2-0.5wt%, eosin 0.001-0.01wt%, the balance is normal saline.

[0031] A further preferred technical solution is that the microtube contains 15-25 µL of antigen solution and 20-50 mg of glass microbeads.

[0032] A further preferred technical solution is that the detection card includes 4-8 microtubes.

[0033] Brucellosis detection card preparation method of the present inve...

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Abstract

The invention discloses a Brucellosis detection card and a preparation method thereof and belongs to the technical field of Brucellosis detection. The Brucellosis detection card comprises 2-10 micro tubes, wherein the micro tubes contain antigen solutions and glass beads; and the antigen solutions are prepared from Brucellosis body fluid, carbolic acid, BSA, casein-sodium, eosine and normal saline. The invention further provides a preparation method of the detection card. The detection card disclosed by the invention is simple in structure, simple and convenient to operate and short in detection period, exogenous heating is not needed, the test results are easily judged, and the result determination is not influenced by subjective factors; and the detection result is convenient to store, and the detection card has extremely high popularization value.

Description

technical field [0001] The invention relates to the technical field of brucellosis detection, in particular to a brucellosis detection card and a preparation method thereof. Background technique [0002] Brucellosis (also known as brucellosis, referred to as brucellosis) is a zoonotic disease caused by Brucella infection. Sick sheep, cattle and other diseased animals are the main source of infection for brucellosis. Brucella can be transmitted through damaged skin and mucous membranes, digestive tract and respiratory tract. Brucellosis is widespread in many countries of the world, with approximately 500,000 new cases worldwide each year. Before the founding of New China, the epidemic was serious. After the founding of New China, special prevention and control institutions were established, and the incidence rate has been significantly reduced. However, since 1994, human and animal brucellosis has rebounded again in our country, and new human and animal brucellosis epidemics...

Claims

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Application Information

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IPC IPC(8): G01N21/82
CPCG01N21/82
Inventor 王振华刘栓江孙杰王云霄
Owner 石家庄久正生物科技有限公司
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