Multivariate volume column chip for visual detection of copper, lead and mercury ions and detection method thereof

Abstract

The invention discloses a multi-volume column chip for visually detecting copper, lead and mercury ions, which comprises a first glass chip and a second glass chip, and an ink moving channel group, an ink indicating channel and a sample groove group are arranged inside the first glass chip , the inner side of the second glass chip is provided with a reagent tank group, and its detection method is also disclosed at the same time, including the preparation of a multivariate volume column chip, the preparation of nucleic acid modified magnetic silica nanoprobes, the preparation of DNA modified platinum nanoprobes, the preparation of composite There are six steps of probe, establishment of standard curve and sample detection; using multi-volume column chip to push the ink column to move through the oxygen generated by the decomposition of hydrogen peroxide, and present the detection result on the chip, without the need for expensive instruments and complicated data processing. It can realize visual and quantitative detection of the concentration of three metal ions of copper, lead and mercury in water, with accurate results, simple operation, low cost and wide application range.

Description

technical field [0001] The invention belongs to the technical field of microfluidic analysis, in particular to a multi-volume column chip for visually detecting copper, lead and mercury ions and a detection method thereof. Background technique [0002] At present, the detection of metal ions in water is mainly based on atomic absorption, atomic emission spectroscopy, and inductively coupled plasma mass spectrometry. These instruments can detect multiple metal ions at the same time, with high detection sensitivity and low detection limit. However, the equipment is expensive and requires high technical skills of the operator, which limits the application of on-site detection and resource-poor areas. [0003] In order to simplify the operation and reduce the cost, a visual detection method based on the metal-specific response of DNA was designed for the detection of metal ions. A series of specific DNA for metal ions can be produced through in vitro screening, which is used fo...

AI Technical Summary

Problems solved by technology

However, these DNA-based visual detection can only give qualitative or semi-quantitative detection results, and the detection limit of the method cannot meet the requirements of the national standard, and instrumental methods are still needed

Moreover, the number of metal ions detected by these methods is too small, which limits the practical application.

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