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Method for establishing renal dysfunction model of mice with IgA nephropathy

A kidney function and mouse technology, which is applied in the field of establishing a model of renal insufficiency in mice with IgA nephropathy, can solve the problem of no report on the model of renal insufficiency, and achieves improvement of hypothermia, high success rate, and good application prospects. Effect

Inactive Publication Date: 2018-12-07
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But there is no report yet about the establishment method of a kind of IgA nephropathy mouse renal insufficiency model of the present invention

Method used

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  • Method for establishing renal dysfunction model of mice with IgA nephropathy
  • Method for establishing renal dysfunction model of mice with IgA nephropathy
  • Method for establishing renal dysfunction model of mice with IgA nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Establishment of IgA Nephropathy Mouse Renal Insufficiency Animal Model (1)

[0041] 1 Materials and methods

[0042] 1.1 Materials

[0043] 1.1.1 Experimental animals

[0044] 40 BALB / c mice were purchased from Shanghai Bikai Company, and the experiment began after one week of adaptive feeding observation in the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.

[0045] 1.2 Method

[0046] 1.2.1 Grouping and administration

[0047] During the observation period, the mice were weighed and numbered, sorted by body weight using IBM SPSS Statistics 23 software, and randomly divided into two groups, 20 mice in each group, and the two groups were the normal control group (Control) and the model group (Model) .

[0048] The mice in the normal control group were given intragastric administration of 0.5 mL of normal saline every day, and the mice in the model group were given oral administration of 0.5 mL of acidified water with 0...

Embodiment 2

[0084] Example 2 Establishment of IgA nephropathy mouse renal insufficiency animal model (2)

[0085] 1 Materials and methods

[0086] 1.1 Materials

[0087] 1.1.1 Experimental animals

[0088] 20 BALB / c mice with similar specifications were purchased from Shanghai Bikai Company, and 20 Kunming mice with similar specifications were observed in the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine for one week after adaptive feeding and observation.

[0089] 1.2 Method

[0090] 1.2.1 Grouping and administration

[0091] During the observation period, the mice were weighed and numbered. There were 20 mice in each group, and the two groups were BALB / c mouse group and Kunming mouse group.

[0092] The mice in the two groups were given 0.5 mL of acidified water with 0.1% BSA orally orally orally every other day, and 0.2 mL of 2% BSA buffer was injected into the tail vein at regular intervals in the sixth week, once a day for 3 consecutive days. ...

Embodiment 3

[0097] Example 3 Establishment of IgA Nephropathy Mouse Renal Insufficiency Animal Model (3)

[0098] 1 Materials and methods

[0099] 1.1 Materials

[0100] 1.1.1 Experimental animals

[0101] 40 BALB / c mice of similar size were purchased from Shanghai Bikai Company, and the experiment began after one week of adaptive feeding observation in the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.

[0102] 1.2 Method

[0103] 1.2.1 Grouping and administration

[0104] During the observation period, the mice were weighed and numbered, sorted by body weight using IBM SPSS Statistics 23 software, and randomly divided into two groups, 20 mice in each group, and the two groups were the normal control group (Control) and the model group (Model) .

[0105]In the model group, 0.5 mL of acidified water with 0.1% BSA was administered orally every other day, and 0.2 mL of 2% BSA buffer solution was injected into the tail vein at regular intervals in the...

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Abstract

The invention relates to a method for establishing a renal dysfunction model of mice with IgA nephropathy. The method comprises the steps of selecting BALB / C mice as animal models; the experiment comprises the steps that 1, an oral low-endotoxin bovine serum albumin acidified aqueous solution is used for intragastric administration every other day for five weeks; 2, in the sixth week, tail veins are injected with 0.2mL of a 2% BSA buffer solution once a day for 3 consecutive days; 3, general states of the mice are observed from the ninth week to the eleventh week; in the 12th week, 0.2mL of the SEB buffer solution is injected into the tail veins at a dose of 0.8 mg / kg per mouse once a week for 3 weeks; the 15th week is the end point of the experiment. The method has the advantages that (1)the method effectively reduces the mortality of the mice with the IgA nephropathy; (2) the model can better simulate the pathogenesis of human IgA nephropathy and further screen for drugs for treating the IgA nephropathy; (3) technical operation is easy and convenient, the animal models have a high success rate, and the method has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of animal models and construction methods, in particular to a method for establishing a renal insufficiency model of IgA nephropathy mice. Background technique [0002] Chronic kidney disease is a global public health problem. According to reports, the prevalence of CKD among adults over the age of 18 in my country is 10.8%. IgA nephropathy (IgA nephropathy, IgAN) is mainly IgA or IgA immunoglobulin Diffuse deposition in the glomerular mesangium and capillary loops, clinical-pathological syndrome with different clinical manifestations and pathological changes, IgA nephropathy is the most common primary glomerular disease, accounting for about 30% of renal diseases The patient was diagnosed with IgA nephropathy after biopsy, which can only be confirmed by renal biopsy. Since Berger first reported IgAN in 1968, scholars from various countries have done a lot of work in the research of IgAN, but the pathogenesis...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61K35/74A61K38/38A61K49/00
CPCA01K67/02A61K35/74A61K38/385A61K49/0008
Inventor 高建东史丽强万强刘伟伟吴燕升林评兰
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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