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Method for evaluating strawberry stamen gray mold resistance

A technique for botrytis and stamens, which is applied in the field of agricultural plant germplasm resource evaluation, and can solve problems such as no public reports.

Active Publication Date: 2018-11-30
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Invented a kind of strawberry stamen to the evaluation method of Botrytis cinerea resistance accordingly, through literature search, do not see the public report of a kind of strawberry stamen identical with the present invention to the evaluation method of gray mold resistance

Method used

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  • Method for evaluating strawberry stamen gray mold resistance
  • Method for evaluating strawberry stamen gray mold resistance
  • Method for evaluating strawberry stamen gray mold resistance

Examples

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Embodiment 1

[0032] A method for evaluating strawberry stamen gray mold resistance, the steps are:

[0033] 1. When strawberry flowers just start to bud, select 10 or 12 or 14 or 16 flowers that have just started to bud for each variety, and pick the flowers and flower stalks from the plant together;

[0034] 2. Insert the flowers into the solidified water agar, and culture them in vitro under constant temperature and light at 25°C for 24 hours, then remove the flowers whose petals have not fully unfolded, and ensure that there are more than 9 flowers with fully unfolded petals for each variety ;

[0035] 3. Use tweezers to remove all the petals from the flower;

[0036] 4, stick the botrytis cinerea conidia suspension with a writing brush, apply the conidia to the anthers of the stamens (the stamens include filaments and anthers, and the anthers here are a part of the stamens) to inoculate; Suspension preparation method: Botrytis cinerea mycelium blocks are inoculated on PDA medium, and...

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Abstract

The invention discloses a method for evaluating strawberry stamen gray mold resistance. The method comprises the steps: A, picking off flowers with stalks from a plant when the strawberry flowers areat the beginning of squaring stage; B, inserting the flowers in solidified water agar, culturing in vitro under an illumination condition; C, picking off petals from the flowers by a tweezer; D, dipping botrytis cinerea conidiospore suspension by a brush pen, coating stamen anther with conidiospore for inoculating; inoculating botrytis cinerea sclertium onto a PDA culture medium; E, re-inserting the flower into the solidified water agar, placing under a constant temperature and illumination condition for culturing in vitro; F, removing young strawberry fruit from sepals, G, measuring the length of the disease spot on filaments, respectively classifying a disease index of each stamen; H, respectively calculating the disease index of each variety; I, classifying the gray mold resistance of each variety according to the disease index of each variety. The method provided by the invention is simple and easy to operate. The method can be used for quickly identifying the gray mold resistanceresistance of sepals of strawberry varieties.

Description

technical field [0001] The invention belongs to the technical field of evaluation of agricultural plant germplasm resources, and more specifically relates to a method for evaluating resistance to botrytis cinerea of ​​strawberry stamens. Background technique [0002] Botrytis cinerea is a kind of important plant pathogenic bacteria widely distributed in the world, which can harm more than 200 host plants such as fruits, vegetables, field crops, and ornamental plants, and cause botrytis cinerea. The above-ground parts of strawberry can be infected with gray mold, especially the flowers and fruits are most seriously damaged by gray mold. Strawberries are very sensitive to Botrytis cinerea within 2 to 3 days after flowering, and the field resistance to gray mold of different strawberry varieties is significantly different. Mildew incidence is very high. The existing resistance evaluation methods can only evaluate the resistance to Botrytis cinerea by observing the field sever...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/645
CPCC12Q1/18G01N2333/37
Inventor 韩永超张庆华曾祥国过聪向发云
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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