Ginsenoside CK preparation method

Abstract

The invention discloses a ginsenoside CK preparation method. According to the present invention, ginsenoside CK is prepared by using a mixed acid hydrolysis method, wherein the ginsenoside CK with thecontent of more than 98% is prepared through preparation of the total saponin of the leaves of Panax notoginseng, preparation of ginsenoside CK, purification and other steps; the conversion efficiency of the method is high, the conversion product is easily separated so as to remove the impurity, and the ginsenoside CK crude product with the content of more than 90% can be obtained by using the simple solvent treatment, such that the disadvantage that the ginsenoside CK can be obtained by repeatedly using the silica gel column and gel column chromatography method is avoided; and the method hasadvantages of low cost and high efficiency, and is suitable for industrial production and marketing application.

Description

technical field [0001] The invention relates to a preparation method of ginsenoside CK, belonging to the field of extract preparation. Background technique [0002] Ginsenoside CK is the metabolite of diol-type ginsenoside in ginseng in the human intestine, and it is the entity that ginseng exerts activity in the body; biological activity studies have shown that ginsenoside CK is a multi-target, highly active compound, which not only has anti-tumor , Anti-inflammation, liver protection and anti-allergic aspects have shown good activities, and also have a good regulatory effect on the nervous system and immune system, and can inhibit tumor cell proliferation and inflammation, anti-carcinogenic and anti-cancer, anti-allergic, relieve Symptoms of Alzheimer's disease, anti-diabetes and antioxidant factors promote the secretion of isulin, protect the liver and skin. [0003] The stability of ginsenoside CK is poor, and ginsenoside does not exist in natural ginseng. The outstandi...

AI Technical Summary

Problems solved by technology

The traditional thinking is to obtain products by using acid-base hydrolysis, but chemical methods have disadvantages such as large pollution, high cost, and many hydrolysis by-products; chemical methods are difficult to control products, and it is difficult to obtain a single target product, and a large number of separations are required in the follow-up process, serious environmental pollution and other disadvantages

Therefore, relevant researches are decreasing year by year, and now they are mostly concentrated on biotransformation methods, mainly including microbial transformation and enzymatic transformation; the conversion efficiency of ginsenoside β-glucosidase isolated from ginseng in literature reports is relatively high, and there are few impurities, but The enzyme is not a single compound, it is difficult to artificially prepare, and the cost of direct separation is high, so it is difficult to apply to the industrialization of the enzymatic hydrolysis preparation method of ginsenoside CK