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A kind of crop straw waste fermentation agent and its preparation method and application

A crop straw and starter technology, applied in the agricultural field, can solve the problems of poor stability of straw waste fermentation effect, unreasonable combination of bacteria and bacteria, and low bacterial content in products, so as to improve the ecological protection environment, The effect of increasing farmers' income and stabilizing the properties of compound structure

Active Publication Date: 2021-06-29
HUBEI WUDANG ANIMAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing starter products, most of the starter products are bacterial agent products developed and produced under stacking conditions, which have the disadvantages of low bacterial count and too small enzyme activity, and are difficult to meet The actual needs of market applications, at the same time, the strains and strain combinations selected for starter products are not reasonable, and the enzyme production ability of the strains themselves and the symbiotic, mutualistic, and antagonistic effects of the strains on the product have not been fully considered. Influenced by the influence, especially there are few types of fungi, and the fermentation effect stability of crop straw waste in use is poor

Method used

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  • A kind of crop straw waste fermentation agent and its preparation method and application
  • A kind of crop straw waste fermentation agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of a crop straw waste starter

[0039] (1) Preparation of Brevibacillus sporogenes powder

[0040] Transfer the freeze-dried Brevibacillus sporogenes to the inclined surface of the test tube in a sterile environment, the temperature is controlled at 30°C, cultivate for 40 hours, and then transfer to the eggplant-shaped bottle with the culture medium for 32 hours. The culture medium in the type bottle is composed of the following components in terms of mass percentage: tryptone 1%, yeast extract 0.5%, sodium chloride 0.5%, the balance is water, pH=7.2.

[0041] Put steam into the fermenter, turn on stirring, keep warm for 30 minutes when the temperature of the medium in the fermenter reaches 121°C, turn off the steam after the sterilization is completed, turn on the cooling system to reduce the temperature of the medium to 32°C, and cultivate in the fermenter The base consists of the following components in terms of mass percentage: 0.05% glucose, 0.05% beef...

Embodiment 2

[0055] Preparation of a crop straw waste starter

[0056] (1) Preparation of Brevibacillus sporogenes powder

[0057] Transfer the freeze-dried Brevibacillus sporogenes to the inclined surface of the test tube in a sterile environment, the temperature is controlled at 32°C, cultivate for 36 hours, and then transfer to the eggplant bottle with the culture medium for 34 hours. The culture medium in the type bottle is composed of the following components in terms of mass percentage: tryptone 1%, yeast extract 0.5%, sodium chloride 0.5%, the balance is water, pH=7.5.

[0058] Put steam into the fermenter, turn on stirring, keep warm for 30 minutes when the temperature of the medium in the fermenter reaches 121°C, turn off the steam after the sterilization is completed, turn on the cooling system to reduce the temperature of the medium to 35°C, and cultivate in the fermenter The base is composed of the following components in terms of mass percentage: 0.01% glucose, 0.07% beef ext...

Embodiment 3

[0072] Preparation of a crop straw waste starter

[0073] (1) Preparation of Brevibacillus lateralsporosa bacteria powder

[0074] Transfer the freeze-dried Brevibacillus sporogenes to the inclined surface of the test tube in a sterile environment, the temperature is controlled at 37°C, cultivate for 24 hours, and then transfer to the eggplant-shaped bottle with the culture medium for 36 hours. The culture medium in the type bottle is composed of the following components in terms of mass percentage: tryptone 1%, yeast extract 0.5%, sodium chloride 0.5%, the balance is water, pH=7.

[0075] Put steam into the fermenter, turn on stirring, keep warm for 30 minutes when the temperature of the medium in the fermenter reaches 121°C, turn off the steam after the sterilization is completed, turn on the cooling system to reduce the temperature of the medium to 30°C, and cultivate in the fermenter The base is composed of the following components in terms of mass percentage: 0.07% gluco...

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Abstract

The invention relates to a crop straw waste starter and its preparation method and application, belonging to the field of agricultural technology. The starter is composed of Brevibacillus spp. Mold powder is composed, and the bacterium concentration of each bacterium in the described fermentation agent is 10 7 -10 9 Cfu / g; the Geotrichum candidum powder is prepared by the following method: After mixing the Geotrichum candidum spore powder and yam powder, inoculate it on the solid medium, and then carry out the bent plate fermentation of the solid medium after the inoculation, the bent plate After the fermentation is finished, it is dried and pulverized to obtain Geotrichum candidum powder. The starter has the characteristics of stable compound structure, small antagonism, strong enzyme production ability, and clear directionality of functional flora. The effect of quickly and thoroughly fermenting crop stalk waste is achieved. Using it in agriculture has good economic and environmental benefits.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a crop stalk waste fermentation agent and a preparation method and application thereof. Background technique [0002] Straw is a kind of biomass energy resource, which is the fourth largest energy after coal, oil and natural gas in the world today, and it is also the only renewable energy. However, most straw resources in my country are burned in the form of fuel, and only a small part is returned to the field through the stomach or directly. The direct burning of straw not only pollutes the environment, but also causes waste of resources. [0003] It is an excellent way for animals to eat straw directly, but direct consumption of straw will cause problems such as low utilization rate of straw, indigestibility, and affecting growth. The use of microbial fermentation can degrade most of the toxic and harmful substances in straw raw materials, and degrade complex ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14A23K10/12C12R1/865C12R1/645C12R1/01
CPCA23K10/12C12N1/14C12N1/18C12N1/20
Inventor 殷素会陈国明熊琛
Owner HUBEI WUDANG ANIMAL PHARMA
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