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Heterotrophic nitrification-aerobic denitrification pseudomonas bacterium agent as well as preparation method and application thereof

A nitrifying Pseudomonas and heterotrophic nitrification technology, applied in the field of sewage treatment, can solve the problems of lack of denitrification process, unsatisfactory total nitrogen removal effect, etc., and achieve the effect of improving effluent quality

Inactive Publication Date: 2018-11-23
中科美大(北京)生态环境工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, since the membrane bioreactor operates under continuous aeration to treat sewage, and lacks the denitrification process, although ammonia nitrogen can be quickly oxidized to nitrate nitrogen, the removal effect of total nitrogen in sewage is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Screening and strain identification of embodiment 1 Pseudomonas W12

[0017] 1) Screening:

[0018] The activated sludge was collected from the sewage treatment plant, stored at 4°C and transported to the laboratory as the screening material for the target bacteria. The heterotrophic nitrifying-aerobic denitrifying bacteria-Pseudomonas W12 was isolated and screened according to the following method:

[0019] ① Preparation of heterotrophic nitrification medium: use analytical reagent and deionized water to prepare heterotrophic nitrification medium, which consists of (NH4) 2 SO 4 2.5g / L, Na 2 HPO 4 12H 2 O 2.0g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.1g / L, sodium citrate 3.3g / L, 0.2% (volume ratio) trace element solution; Wherein the trace element solution consists of EDTA-Na 2 50g / L, ZnSO 4 2.2g / L, CaCl 2 5.5g / L, MnCl 2 4H 2 O 5.1g / L, FeSO 4 ·7H 2 O 5.0g / L, CuSO 4 ·5H 2 O1.6g / L, CoCl 2 ·6H 2 O1.6g / L; the pH range of the heterotrophic nitrification ...

Embodiment 2

[0027] The preparation of embodiment 2 Pseudomonas W12 inoculum

[0028] As an embodiment of the present invention, the preparation method of described Pseudomonas W12 bacterial agent comprises the following steps:

[0029] 1) Preparation of Pseudomonas W12 liquid culture medium: get 4.5 kilograms of ammonium sulfate, 0.25 kilograms of disodium hydrogen phosphate, 1 ton of sweet potato starch waste whose COD concentration is 45g / L, adjust the pH of the medium to 7.8;

[0030] 2) Cultivation of Pseudomonas W12 liquid seeds: the Pseudomonas W12 liquid medium prepared by step 1) is packed into an Erlenmeyer flask, and the slanted-plane preservation is continued to be inoculated in the Erlenmeyer flask containing this liquid medium. Pseudomonas W12 was shaken at 28°C and 180 rpm for 22 hours to obtain Pseudomonas liquid seeds;

[0031]3) Preparation of Pseudomonas W12 liquid bacterial agent: the Pseudomonas W12 liquid medium prepared by step 1) is packed into a fermenter, and th...

Embodiment 3

[0032] The preparation of embodiment 3 Pseudomonas W12 microbial agent

[0033] As another embodiment of the present invention, the preparation method of the Pseudomonas W12 bacterial agent comprises the following steps:

[0034] 1) Preparation of Pseudomonas W12 liquid medium: get 5 kg of ammonium sulfate, 0.3 kg of disodium hydrogen phosphate, and 1 ton of sweet potato starch waste with a COD concentration of 30 g / L, and adjust the pH of the culture medium to 7.5;

[0035] 2) Cultivation of Pseudomonas W12 liquid seeds: the Pseudomonas W12 liquid medium prepared by step 1) is packed into an Erlenmeyer flask, and the slanted-plane preservation is continued to be inoculated in the Erlenmeyer flask containing this liquid medium. Pseudomonas W12 was shaken and cultured at 30°C and 150 rpm for 24 hours to obtain Pseudomonas liquid seeds;

[0036] 3) Preparation of Pseudomonas W12 liquid bacterial agent: the Pseudomonas W12 liquid medium prepared by step 1) is packed into a ferm...

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Abstract

The invention discloses a heterotrophic nitrification-aerobic denitrification pseudomonas bacterium agent as well as a preparation method and application thereof. The heterotrophic nitrification-aerobic denitrification pseudomonas bacterium agent is a pseudomonas sp. W12 strain and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation location is Institute of Microbiology, Chinese Science Academy, #3, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing; the registered preservation number is CGMCC No. 12460 and the preservation date is May 17, 2016. The heterotrophic nitrification-aerobic denitrification pseudomonas bacterium agent takes sweet potato wastewater as a main culture medium and the W12 strain is inoculated; air is introduced to culture the strain to obtain a pseudomonas W12 liquid bacterium agent. After a proper amount of the pseudomonas W12 liquid bacterium agent is added, the average removal rate of a membrane bioreactor onammonia nitrogen is remarkably improved, and the average removal rate on total nitrogen is more remarkably improved, so that the water quality of output water of the membrane bioreactor is improved.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a heterotrophic nitrification-aerobic denitrification Pseudomonas sp. Applications. Background technique [0002] Membrane Bio-Reactor (MBR for short) is a high-efficiency sewage treatment technology that combines a membrane separation unit with a biological treatment unit. It replaces the secondary settling tank with membrane components and maintains highly active sludge in the bioreactor. Concentration, increase the organic load of biological treatment, reduce the land occupation of sewage treatment facilities, and reduce the amount of sludge by keeping the sludge load low. Compared with traditional biochemical water treatment technology, membrane bioreactor has the following advantages: high treatment efficiency, good effluent quality; compact equipment, small footprint; easy to realize automatic control, convenient operation and management. [0003] However, since ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/38
CPCC02F3/34C02F2101/16C12N1/20
Inventor 张剑韩振飞吕鹏翼夏丛伟郑美大白志辉
Owner 中科美大(北京)生态环境工程有限公司
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