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Coix lacryma fermentation extract and preparation method and application thereof

An extract, the technology of barley, applied in the field of fermented barley extract and its preparation, can solve the problems of low technical efficiency and achieve the effect of maintaining natural activity, small molecular weight, and mild fermentation pH

Inactive Publication Date: 2018-11-23
上海仪玳化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Designed to address the inefficiencies of existing extraction techniques

Method used

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  • Coix lacryma fermentation extract and preparation method and application thereof
  • Coix lacryma fermentation extract and preparation method and application thereof
  • Coix lacryma fermentation extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The concentration of the fermentation bacteria in the expansion medium was 1o 5 -10 6 Inoculate 8mL of CFU / ml fermented bacteria liquid into 30 mesh 10g barley powder and 300mL water, and ferment in a shaker at 28°C for 40 hours, then autoclave the obtained fermentation liquid at 115°C for 20min , to inactivate the bacteria, centrifuge the sterilized fermented liquid for 20 min under the conditions of 12000 r / min and a centrifugal radius of 9 cm, and collect the supernatant, which is the barley fermented extract provided by the present invention.

[0046] The barley fermented extract prepared in this example can be directly used as a cosmetic, and its appearance is a viscous liquid, and its color is light pink to brownish red. The pH value is 5.2-6.8, the viscosity is 200-500cP, the soluble solid content is 2-5.0%, the total number of colonies is less than 50CFU / ml, no pathogenic bacteria are detected, and it meets the quality requirements of cosmetics.

[0047] The c...

Embodiment 2

[0052] DPPH is an early synthesized organic free radical, which is often used to evaluate the hydrogen-donating ability of antioxidants. It is very stable in organic solvents, has a purple color, and has a characteristic absorption peak at , when it encounters a free radical scavenger , the lone pair of electrons of DPPH is paired to make it fade, that is, the light absorption value at the maximum absorption wavelength becomes smaller. Therefore, the scavenging effect of the sample on DPPH free radicals can be evaluated by measuring the change of the absorbance value.

[0053] The specific experimental steps of the DPPH free radical scavenging experiment are as follows:

[0054] (1) Take an equal volume (usually 3mL) of the solution to be tested and 2×10 -4 mol / L DPPH solution mixed (A 1 Tube);

[0055] (2) Take an equal volume of absolute ethanol (the solvent for the analyte) and 2×10 -4 mol / L DPPH solution mixed (A 2 Tube);

[0056] (3) Take an equal volume of absolute...

Embodiment 3

[0061] Tyrosinase is the key enzyme of melanin production, it controls the formation process of melanin, and its activity level plays a major role in the deposition of pigment. Many whitening and freckle-removing products currently on the market achieve whitening effects by inhibiting tyrosinase, so the strength of the inhibitory effect on tyrosinase is the main indicator for evaluating whitening cosmetics.

[0062] Evaluate the whitening function of the sample by measuring the impact of the sample on tyrosinase, the specific method is as follows:

[0063] Configure the solution according to the ratio shown in Table 2:

[0064] Table 2, solution preparation list

[0065] Unit (mL)

C 1

C 2

T 1

T 2

L-tyrosine

2

2

2

2

sample

0

0

2

2

PBS

4

5

2

3

Tyrosinase

1

0

1

0

total capacity

7

7

7

7

[0066] Note: C 1 and T 1 Add 1mL of tyrosinase, the enzyme activit...

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Abstract

The invention relates to the technical field of make-ups, in particular to a coix lacryma fermentation extract and a preparation method thereof. The method comprises the following steps that blendingis conducted, wherein a ratio of zymophyte to coix lacryma powder to water is (5 to 10 mL) to (10 to 15 g) to (250 to 350 mL); an initial system is prepared, wherein the coix lacryma powder, the zymophyte and the water are fully mixed to obtain the initial system, and the concentration of the zymophyte in the initial system is 105-106 CFU / ml; fermentation is conducted, wherein the initial system is subjected to fermentation treatment to obtain fermentation liquid; a finished product is obtained, wherein after the fermentation liquid is subjected to sterilization and centrifugal treatment in sequence, supernate is collected, and the coix lacryma fermentation extract is obtained; the coix lacryma powder is powder of coix lacryma root; yeast liquid is adopted as the zymophyte. Compared with the prior art, the coix lacryma fermentation extract and the preparation method thereof have the advantages that the natural activity of plants is effectively maintained, and the safety of the productis improved for a human body.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a barley fermented extract and its preparation method and application. Background technique [0002] Barley is a plant of the Gramineae family of Job's tears, which is mainly produced in the vast areas south of 33°N. The seeds, roots, stems and leaves of Job's tears can all be used by humans, and its nutritional value and medicinal value are unmatched by other plants. Therefore, it is known as "the king of grasses in the world". The Ministry of Health has now listed Job's tears as both food and medicine. [0003] At present, the cosmetics containing barley ingredients in the market all add a certain proportion of barley extract to the basic formula of the cosmetics. cosmetic effect. [0004] Therefore, the prior art has yet to be developed. Contents of the invention [0005] In view of the deficiencies in the prior art above, the object of the present invention is to prov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9794A61Q19/00A61Q19/02A61Q19/08
CPCA61Q19/00A61Q19/02A61Q19/08A61K8/9794A61K2800/782A61K2800/85
Inventor 方国忠
Owner 上海仪玳化妆品有限公司
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