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Culture medium and method for inducing generation of macleaya cordata embryoid callus

A technology of callus and medium, applied in the field of plant tissue culture, to shorten the growth period, improve the culture efficiency, and speed up the culture speed.

Active Publication Date: 2018-11-23
MICOLTA BIORESOURCE INC LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there have been no reports of its application in plant tissue culture.

Method used

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  • Culture medium and method for inducing generation of macleaya cordata embryoid callus
  • Culture medium and method for inducing generation of macleaya cordata embryoid callus
  • Culture medium and method for inducing generation of macleaya cordata embryoid callus

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Embodiment Construction

[0055] The present invention will be further described in detail through the following experimental cases, but the content of the present invention is not limited thereto.

[0056] 1. The preparation of the culture medium, first, the MS medium powder bought on the market, according to the standard ratio, 4.42 grams of MS medium powder and 1L of water are adjusted to the basic medium, then add sucrose: 30g / L+ plant gel: 3g / L, adjust the pH value to 5.8 to obtain MS medium;

[0057] Then prepare different culture media required for different stages, and mark them separately. The culture media and their composition are shown in Table 1 below:

[0058] Table 1 Medium number and corresponding formula table

[0059]

[0060]

[0061] 2. Experimental operation

[0062] 2.1 Selection of explants: Take fresh and healthy leaves and twigs, and bring them back to the laboratory in an ice box for future use.

[0063] 2.2 Sterilization of explants: First, use scissors to subtract t...

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Abstract

The invention provides a culture medium and method for inducing generation of macleaya cordata embryoid callus, and belongs to the technical field of plant tissue culture. The culture medium for inducing generation of the macleaya cordata embryoid callus is prepared from an MS culture medium and additives 2,4-D, IAA (indole acetic acid), KT (kinetin) and PQQ (pyrroloquinoline quinone), wherein theconcentration of the MS culture medium is 4.42g / L, the concentration of 2,4-D is 0.2-0.6 micromole / L, the concentration of IAA is 4.0-6.5 micromole / L, the concentration of KT is 0.2-0.6 micromole / L,and the concentration of PQQ is 100-1,000nmol / L. The method comprises the following steps that (1) an explant is selected and sterilized; (2) the above culture medium is adopted to induce culture of embryoid callus; (3) the embryoid is induced and cultured; (4) somatic embryogenesis germinates; (5) a grown-up seedling is cultured; (6) a regrowth seedling is transplanted; and (7) the regrowth seedling is cultured in a greenhouse. The explant is selected from stems or leaves of macleaya cordata, PQQ is added into the culture medium on the basis of common plant hormones, the culture medium and the method can achieve the aim that the tissue culture seedlings of macleaya cordata can be rapidly cultured with high yield, can greatly shorten the seedling time of macleaya cordata, and are applicable to large-area production.

Description

technical field [0001] The present invention belongs to the technical field of plant tissue culture of syringae, specifically a culture medium and method for inducing scorpion embryoid callus, especially relates to a somatic cell rapid induction of scorpion embryoid callus A method of producing plant regeneration. Background technique [0002] bo fall back [Macleaya cordata(Willd) R .B] is a perennial plant of the genus Papaveraceae, which is an important traditional medicinal plant. The active ingredients are mainly isoquinoline alkaloids, mainly including sanguinarine (SA), chelerythrine (chelerythrine, CHE), protopine (PRO) and allocryptopine (ALL), etc., have significant biological activities such as anti-inflammatory, anti-tumor, improving liver function, and regulating gastrointestinal tract. Boluohui extract has been approved as my country's first second-class new veterinary drug feed additive [(2011) New Veterinary Drug Zhengzi No. 33, No. 34]. Since January 2006,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾建国余林岚
Owner MICOLTA BIORESOURCE INC LTD
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