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Laser desorption ionization mass spectrometry detection method

An ionization mass spectrometry and detection method technology, applied in the field of laser desorption ionization mass spectrometry detection, can solve the problems of complex preparation process, generation of molecular fragments, short service cycle, etc. Effect

Inactive Publication Date: 2018-11-20
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems of low ionization efficiency, easy generation of molecular fragments, high production cost, complicated preparation process and short service life of the existing inorganic materials used as laser desorption ionization sample loading substrates, the present invention provides a high-efficiency light-absorbing Materials as a method for loading samples in laser desorption ionization

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  • Laser desorption ionization mass spectrometry detection method

Examples

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Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Using a light-absorbing material with an absorbance of 80% as the LDI mass spectrometry analysis of the substrate

[0048] Preparation of analytical samples: prepare a reserpine solution with a concentration of 1 mM, the solvent is methanol / water 3 / 7, and place it in a 4°C refrigerator for later use.

[0049] LDI mass spectrometry analysis:

[0050] (1) Prepare a light-absorbing metal material with an absorbance rate of 80% on copper.

[0051] (2) Clean the material with an organic solvent, dry it thoroughly in the air, fix it on the target plate and use it as a sample loading substrate, take 1 μL of reserpine solution and drop it on the surface of the material and dry it.

[0052] (3) The target plate was placed in a MALDI-TOF mass spectrometer for laser desorption ionization mass spectrometry analysis.

[0053] (4) After the test, the light-absorbing material was ultrasonically cleaned with organic solvents and water and dried, and kept for the next use. ...

Embodiment 2

[0055] Embodiment 2 uses conventional MALDI (substrate used is DHB) and uses the comparison of the LDI mass spectrometry analysis that uses high-efficiency light-absorbing material to do substrate

[0056] Preparation of matrix solution: DHB solution with a concentration of 20 mg / mL was prepared, and the solvent was acetonitrile / water 3 / 7.

[0057] Preparation of analytical samples: Glucose was prepared as a deionized aqueous solution with a concentration of 1 mM, and placed in a refrigerator at 4° C. for later use.

[0058] Conventional MALDI mass spectrometry analysis of samples: take 1 μL matrix DHB solution and spot it on a commercial stainless steel target plate, after it dries to form a matrix thin layer, take the same volume of glucose solution and drop it on top of the thin layer. After being dried naturally, it was put into a MALDI-TOF mass spectrometer for mass spectrometry analysis. Carry out 5 repeated experiments, calculate its main ion peak ([M+Na] + ) average ...

Embodiment 3

[0065] Example 3 Using high-efficiency light-absorbing copper material as a sample-loading substrate for laser desorption ionization mass spectrometry analysis of neutral sugar mixtures

[0066] Preparation of analysis samples: Lactose, maltotriose, maltopentaose, β-cyclodextrin and γ-cyclodextrin were respectively configured as deionized aqueous solutions with a concentration of 1mM, and equal volumes of the five solutions were mixed to obtain a mixture solution , placed in a 4°C refrigerator for later use.

[0067] LDI mass spectrometry analysis using high-efficiency light-absorbing copper material as the sample-loading substrate:

[0068] (1) Prepare high-efficiency light-absorbing metal materials on copper, and the light-absorbing rate is 95%.

[0069] (2) Clean the material with an organic solvent, dry it thoroughly in the air, fix it on the target plate and use it as a sample loading substrate, take 2 μL of the sugar mixture solution and drop it on the surface of the ma...

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Abstract

The invention discloses a laser desorption ionization mass spectrometry detection method. The method includes the following steps: (1) preparing an efficient light absorbing material as a sample substrate; (2) dropping a to-be-detected sample into the sample substrate, and drying the to-be-detected sample; and (3) performing laser desorption ionization mass spectrometry analysis. The desorption ionization of organic and biomolecules can be effectively realized by using the efficient light absorbing material as the sample substrate of laser desorption ionization, and fragment ions are hardly generated; mass spectrometry detection and analysis on various samples such as carbohydrate, amino acid, metabolite and drug molecules can be realized; and good signal to noise ratio, repeatability, high sensitivity and high salt tolerance can be realized; through the method, complex sample preparing processes are not needed, the addition of organic matrixes are not needed, and there is no background peak interference at low quality end; and a general, simple, efficient, fast and sensitive soft ionization detection method can be provided for various biomolecules.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry, in particular to a laser desorption ionization mass spectrometry detection method using a high-efficiency light-absorbing material as a sample loading substrate. Background technique [0002] Matrix-assisted laser desorption / ionization (MALDI) has been extensively developed since it was proposed in the late 1980s. Due to the characteristics of high sensitivity, high accuracy, fast detection speed and wide range of mass, MALDI has special advantages in the application of determination of biological macromolecules and high polymers. A powerful tool for nucleotides and polymers. The key to MALDI analysis is the introduction of an organic matrix that can effectively absorb laser light. After the matrix co-crystallizes with the analyte, it absorbs the energy of the laser pulse and transfers it to the analyte, so that the analyte can be desorbed and ionized. The currently commonly used subs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 欧阳证杨静钟毓霖
Owner TSINGHUA UNIV
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