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Method for preparing sandwich type photoelectric chemical sensor for cardiac troponin I

A cardiac troponin and photoelectrochemical technology, which is applied in the field of preparation of sandwich-type photoelectrochemical sensors, can solve the problems of high requirements for testing personnel, low detection sensitivity, and complicated steps, and achieve the effects of low price, large surface area, and improved conductivity

Active Publication Date: 2018-11-16
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many detection methods for cardiac troponin I, these methods have complicated steps, long time consumption, low detection sensitivity and high requirements for detection personnel.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 A kind of preparation method of the sandwich type photoelectrochemical sensor of cardiac troponin I

[0037] (1) The 2.5 cm × 0.8 cm ITO conductive glass was ultrasonically cleaned with detergent, acetone, absolute ethanol and ultrapure water in sequence, and dried with nitrogen;

[0038] (2) Take 8 µL, 8 mg / mL nanometer square titanium dioxide TiO 2 Add the suspension dropwise to the conductive surface of the conductive glass electrode, and let it dry naturally at room temperature;

[0039] (3) on TiO 2 On the modified electrode surface, 4 µL of carboxylated CdS quantum dot solution was added dropwise, dried at room temperature, and the electrode surface was rinsed with ultrapure water to obtain TiO 2 / CdS electrode;

[0040] (4) on TiO 2 Add 4 μL, 0.1 mol / L mercaptoacetic acid dropwise to the surface of the CdS electrode, let it dry at room temperature, and then add 4 μL 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide dropwise / N-hydroxysuccinimide, r...

Embodiment 2

[0046] Embodiment 2 A kind of preparation method of the sandwich type photoelectrochemical sensor of cardiac troponin I

[0047] (1) The 2.5 cm × 0.8 cm ITO conductive glass was ultrasonically cleaned with detergent, acetone, absolute ethanol and ultrapure water in sequence, and dried with nitrogen;

[0048] (2) Take 9 µL, 10 mg / mL nanometer square sheet of titanium dioxide TiO 2 Add the suspension dropwise to the conductive surface of the conductive glass electrode, and let it dry naturally at room temperature;

[0049] (3) on TiO 2 On the modified electrode surface, 5 µL carboxylated CdS quantum dot solution was added dropwise, dried at room temperature, and the electrode surface was rinsed with ultrapure water to obtain TiO 2 / CdS electrode;

[0050] (4) on TiO 2 Add 5 µL, 0.1 mol / L mercaptoacetic acid dropwise to the surface of the / CdS electrode, let it dry at room temperature, and then add 5 µL of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide dropwise / N-hydroxysuc...

Embodiment 3

[0056] Embodiment 3 A kind of preparation method of the sandwich type photoelectrochemical sensor of cardiac troponin I

[0057] (1) The 2.5 cm × 0.8 cm ITO conductive glass was ultrasonically cleaned with detergent, acetone, absolute ethanol and ultrapure water in sequence, and dried with nitrogen;

[0058] (2) Take 10 µL, 12 mg / mL nanometer square sheet of titanium dioxide TiO 2 Add the suspension dropwise to the conductive surface of the conductive glass electrode, and let it dry naturally at room temperature;

[0059] (3) on TiO 2 On the modified electrode surface, 5 µL carboxylated CdS quantum dot solution was added dropwise, dried at room temperature, and the electrode surface was rinsed with ultrapure water to obtain TiO 2 / CdS electrode;

[0060] (4) on TiO 2 Add 5 µL, 0.1 mol / L mercaptoacetic acid dropwise to the surface of the / CdS electrode, let it dry at room temperature, and then add 5 µL of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide dropwise / N-hydroxysu...

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Abstract

The invention relates to a method for preparing a sandwich type photoelectric chemical sensor for cardiac troponin I and belongs to the technical field of nano functional materials, immunoassay and photoelectrochemistry sensation. According to the method, a carboxylation CdS quantum dot is adopted to sensitize a 001 crystal surface of a nano square piece TiO2, then visible light absorption can beimproved, a composite material TiO2 / CdS of which the photoelectric activity is remarkably improved is prepared, by using a layer-by-layer self-assembling method, a cardiac troponin I antibody, bovineserum albumin and cardiac troponin I antigen are assembled on the composite material TiO2 / CdS, Ag@Cu2O shell-core nanoparticles are adopted as a second antibody marker, and by virtue of the excellentphotoelectric activity of the TiO2 / CdS and specific combination of antigen and antibodies of the cardiac troponin I, super-sensitive detection on the cardiac troponin I can be achieved, and the methodhas great significances for analysis and detection on the cardiac troponin I.

Description

technical field [0001] The invention belongs to the technical fields of nanometer functional materials, immune analysis and photoelectrochemical biosensing, and provides a preparation method of a sandwich type photoelectrochemical sensor of cardiac troponin I. Specifically, the use of carboxylated CdS quantum dots to modify nano-square TiO 2 The 001 crystal facet of the antibody was used as the antibody capture substrate, using Ag@Cu with dual inhibition 2 O core-shell nanoparticles were used as secondary antibody markers to prepare a sandwich-type photoelectrochemical sensor for detecting cardiac troponin I-specific antigens. Background technique [0002] Cardiovascular disease is the number one killer threatening human health in the world today, and acute myocardial infarction has the characteristics of rapid onset, high disability rate, and high mortality, so the research on acute myocardial infarction has always been a hot spot. Cardiac troponin is an internationally r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 孔玲陈杰陈志伟孙晓凯
Owner SHANDONG UNIV OF TECH
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