A kind of ultra-low molecular weight dendrobium officinale polysaccharide and its preparation and application

A Dendrobium officinale polysaccharide and Dendrobium officinale technology, which is applied in the direction of medical preparations containing active ingredients, organic active ingredients, preparations for toiletry, etc., can solve the problems affecting the structure and activity of polysaccharides, harmful to human health, and organic solvent residues. To meet the requirements of safe and non-toxic, mild extraction conditions, and excellent transdermal absorption

Active Publication Date: 2021-02-09
山东朵拉朵尚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the acid-base method is easy to break some glycosidic bonds of polysaccharide molecules, which seriously affects the structure and activity of polysaccharides.
Sevage method, trichloroacetic acid method, trifluorotrichloroethane method and other methods use a lot of toxic chemical reagents. These methods inevitably have the problem of organic solvent residue, which is not only harmful to human health, but also has potential safety hazards.

Method used

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  • A kind of ultra-low molecular weight dendrobium officinale polysaccharide and its preparation and application
  • A kind of ultra-low molecular weight dendrobium officinale polysaccharide and its preparation and application
  • A kind of ultra-low molecular weight dendrobium officinale polysaccharide and its preparation and application

Examples

Experimental program
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Effect test

preparation example Construction

[0053] The preparation step of PDA solid medium in the embodiment is: fresh potato is washed and peeled, takes by weighing 200g potato and cuts into pieces, adds water and boils (boiling 30min), utilizes 300 order filter cloth to filter residue, adds agar 20g in filtrate, Glucose 20g, add water to 1L, sterilize at 120°C for 20min, cool down and store for later use.

[0054] The configuration method of Sabouraud's liquid medium is: 40.0g glucose, 10.0g peptone are dissolved in artificial seawater (artificial seawater formula: NaCl 20g, MgSO 4 ·7H 2 O 1g, CaCl 2 2H 2 O 1g, KCl 0.5g, NaHCO 3 0.2g, KBr0.1g, H 3 BO 3 0.01mg, distilled water was added to 1L), and the volume was adjusted to 1L, and then sterilized at 120°C for 20min and stored for later use.

[0055] The disposition method of described nichloramine 18% glycerin medium is: peptone 5.0g, anhydrous glucose 10.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, nicloramine 0.002g, chloramphenicol 0.1g, glycerin 200g, di...

Embodiment 1

[0057] (1) Activation and expansion of strains: Dissolve Aspergillus tubingensis in sterile water to make a bacterial suspension, pipette 3 μL of the bacterial suspension and streak on the surface of the PDA solid medium, activate the culture, and cultivate at a temperature of 30°C. Time 48h, obtain the activated Aspergillus tubingensis bacterial classification (concentration after activation is 1 * 10 4 CFU / mL); Inoculate the activated Aspergillus tubingensis strain into a fermentation shaker flask containing Sabouraud's liquid medium with 1% inoculum amount to carry out expansion cultivation. The cultivation temperature is 30° C. and the rotation speed is 150 r / min. , to obtain the fermented seed liquid of Aspergillus tubingensis, the concentration of the strain was 1×10 7 CFU / mL;

[0058] (2) raw material processing of Dendrobium candidum: take 4kg fresh Dendrobium candidum, pulverizer breaks up, juice is put into 100L fermenter together with slag, injects 96kg Sabouraud l...

Embodiment 2

[0063] (1) Strain activation and expansion: Dissolve Aspergillus tubingensis in sterile water to make a bacterial suspension, pipette 4 μL of the bacterial suspension in DRBC agar medium for activation, culture temperature 28 ° C, culture time 60 h, to obtain The activated Aspergillus tubingensis species (concentration of 1.5 × 10 5 CFU / mL); Inoculate the activated Aspergillus tubingensis strain into the fermentation shaker flask containing Sabouraud liquid medium with 1% inoculum amount to carry out expansion culture, the culture temperature is 28 ℃, the rotating speed 170r / min, after cultivating 60h , to obtain the fermented seed liquid of Aspergillus tubingensis, the concentration of the strain was 1.0×10 8 CFU / mL;

[0064] (2) Dendrobium candidum raw material processing: take 5kg fresh Dendrobium candidum, pulverizer breaks up, juice is put into fermenter together with slag, injects 95kg Sabouraud's liquid culture medium, makes the mass fraction of Dendrobium candidum be ...

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Abstract

The invention belongs to the technical field of dendrobium polysaccharide preparation, and discloses an ultra-low molecular weight dendrobium officinale polysaccharide and its preparation and application. Method: (1) Put the activated Aspergillus tabiensis species into the liquid seed medium for cultivation to obtain the seed liquid; (2) Take the seed liquid, inoculate it into the fermentation medium of the fermenter, and adjust the fermenter The pH of the feed liquid is 4.0 to 7.0, fermented at 25 to 35°C to obtain a fermented liquid; the fermentation medium contains broken Dendrobium candidum; (3) inactivating the fermented liquid; (4) inactivating the inactivated The fermented liquid is purified, concentrated, added with excipients, homogenized under high pressure, and dried to obtain the polysaccharide of Dendrobium officinale. The method of the present invention is simple, safe, and pollution-free, and the yield of polysaccharides is high, and the purity is high; among the polysaccharides obtained from Dendrobium nobile polysaccharides, the polysaccharides with a weight-average molecular weight less than 10 kDa account for more than 98% of the total polysaccharides, so the polysaccharides from Dendrobium obtained from the present invention belong to Dendrobium polysaccharides with ultra-low molecular weight.

Description

technical field [0001] The invention belongs to the technical field of dendrobium polysaccharide preparation, and in particular relates to an ultra-low molecular weight dendrobium officinale polysaccharide and its preparation method and application. Background technique [0002] Dendrobium officinale kimura et Migo is a perennial epiphytic herb of the genus Dendrobium orchidaceae, rich in polysaccharides, dendrobines, polyphenols, phenanthrenes, joints, xenone compounds, amino acids and trace elements, etc. Bioactive substances are traditional Chinese herbal medicines in my country. According to phytochemical and phytopharmacological studies, the main active ingredient of Dendrobium candidum is polysaccharides, which have the functions of immune regulation, anti-tumor, anti-fatigue, anti-allergic and anti-inflammatory, anti-oxidation, anti-aging, and moisturizing. Based on the excellent biological activity of Dendrobium officinale polysaccharides, Dendrobium polysaccharides ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04A23L33/125A61K8/60A61K31/715C12R1/66
CPCA61K8/60A61K31/715A23L33/125C12P19/04
Inventor 周心慧程建华黄桂州周健邓燕柠付尉芳张定宇张继勇
Owner 山东朵拉朵尚生物科技有限公司
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