Microorganism calcareous glue, preparation method and application thereof, sandy soil column and preparation method thereof
A technology of microorganisms and microbial bacteria liquid, applied in the fields of microbial calcareous glue and preparation, application, sand column and preparation, can solve the problem of high brittleness of the solidified sand column, and achieve the improvement of cohesion, contact force, and increase in The effect of a large angle of internal friction
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Embodiment 1
[0049] This embodiment provides a green microbial calcareous glue, which is mainly used for sandy soil consolidation. Knot.
[0050] The main components of the green microbial calcareous gel are mineralized microbial bacterial liquid, calcium source nutrient solution, and green colloid, mineralized microbial bacterial liquid, calcium source nutrient solution, and green gelatin. The volume ratio of the three is 1: (10-100): (0.001-1).
[0051] When the microbial bacterial liquid was prepared, the strain of the microbial bacterial liquid was Bacillus sarcina pasteurii (Sporosarcina pasteurii, ATCC11859), and the bacillus sarcina pasteurii was inoculated to the The culture medium was shaken and expanded to make a bacterial liquid.
[0052] Among them, the medium components are: 10~30g / L yeast powder, 2~10g / L NH 4 SO 4 , 0~15.748g / LC 4 h 11 NO 3 , 0~3g / L NiC l2 ; Remove the original medium from the bacterial solution after shaking and expansion, and then dilute it with fre...
Embodiment 2
[0059] This embodiment provides the preparation method of the green microbial calcareous gel of embodiment 1. The preparation method of the green microbial calcareous gel comprises the following steps.
[0060] Step 1, prepare the microbial bacterial liquid of mineralization performance
[0061] The strain of the microbial bacterial liquid is Bacillus sarcinis, and the bacillus sarcinis is inoculated into the culture medium according to the inoculum amount of 5%, and the culture medium is shaken and propagated to make the bacterial liquid, wherein, the culture medium The ingredients are: 10~30g / L yeast powder, 2~10g / L NH 4 SO 4 , 0~15.748g / L C 4 h 11 NO 3 , 0~3g / L NiC l2 .
[0062] Remove the original medium from the bacterial solution after shaking and expansion, and then dilute it with fresh medium, when the OD of the bacterial solution is 600 When the value is 0.5-1.0, the dilution is stopped, and the obtained bacterial liquid is the microbial bacterial liquid. The...
Embodiment 3
[0068] This embodiment discloses the method for using the green microbial calcareous glue in embodiment 1 in consolidating sandy soil, which includes the following steps.
[0069] Step 1: Prepare the microbial bacterial liquid with mineralization performance: the strain of the microbial bacterial liquid with mineralization performance is Bacillus pasteurii (Sporosarcina pasteurii, ATCC11859), which is inoculated into the culture medium according to the inoculum size of 5% and shaken Expand culture to make bacterial liquid, in which the medium components are 10-30g / L yeast powder, 2-10g / L NH 4 SO 4 , 0~15.748g / L C 4 h 11 NO 3 , 0~3g / L NiCl 2 , the bacterial solution after shaking and expansion was centrifuged in a centrifuge at 30°C and 200rpm for 20 minutes, the original medium was removed, and the Bacillus sarcina was diluted with fresh medium to remove the original medium. OD 600value (the absorbance value of the solution at a wavelength of 600nm, which is often used t...
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