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Paracoccus denitrificans strain with sulfur and ferrum co-substrate autotrophic denitrification function and culture method and application thereof

A denitrification paracoccus, autotrophic denitrification technology, applied in the denitrification paracoccus strain, the application field of wastewater denitrification treatment process, can solve problems such as pollution, achieve the effect of simple process, low cost and strong practical value

Active Publication Date: 2018-11-16
DALIAN UNIV OF TECH
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AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of nitrate and nitrite pollution in wastewater, to provide a bacterium with autotrophic denitrification function of sulfur-iron co-substrate, to remove industrial denitrification under the condition of no external carbon source or insufficient carbon source. Nitrates and Nitrites in Wastewater, Domestic Sewage and Groundwater

Method used

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  • Paracoccus denitrificans strain with sulfur and ferrum co-substrate autotrophic denitrification function and culture method and application thereof
  • Paracoccus denitrificans strain with sulfur and ferrum co-substrate autotrophic denitrification function and culture method and application thereof

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Embodiment 1

[0016] Embodiment 1: bacterial strain of the present invention removes nitrate in waste water with pyrite as electron donor under anaerobic conditions

[0017] Denitrification medium: KH 2 PO 4 14.7mmol / L, NaHCO 3 30mmol / L, NH 4 Cl 18.7mmol / L, MgCl 2 ·6H 2 O 3.25mmol / L, CaCl 2 2H 2 O 0.05mmol / L, pyrite 500mmol / L, adjust the pH to 7.0-7.2, and the medium is sterilized before use.

[0018] Using denitrification medium, inoculate the strain ZGL1 with a dry weight of 0.8g / L under anaerobic conditions, cultivate it at 30°C, and take samples at regular intervals to determine the changes in the concentration of nitrate and nitrite. The results are shown in Table 1. It can be seen from Table 1 that under anaerobic conditions, with pyrite as the electron donor, nitrate can be completely degraded, and after 30 hours of cultivation, 100% of the nitrate in the system was removed. Nitrite accumulated in the middle process and was completely converted into nitrogen after 102 hours...

Embodiment 2

[0021] Embodiment 2: Bacteria of the present invention removes nitrate in waste water with ferrous sulfide as electron donor under anaerobic conditions

[0022] Denitrification medium: KH 2 PO 4 14.7mmol / L, NaHCO 3 30mmol / L, NH 4 Cl 18.7mmol / L, MgCl 2 ·6H 2 O 3.25mmol / L, CaCl 2 2H 2 O 0.05mmol / L, ferrous sulfide 500mmol / L, adjust the pH to 7.0~7.2, and the medium is sterilized before use

[0023] Using the denitrification medium, inoculate the strain ZGL1 with a dry weight of 0.8g / L under anaerobic conditions and cultivate it at 30°C. Samples were taken at regular intervals to determine the changes in the concentration of nitrate and nitrite. The results are shown in Table 2. It can be seen from Table 2 that under anaerobic conditions, with ferrous sulfide as the electron donor, nitrate can be completely degraded, and after 24 hours of cultivation, 100% of the nitrate in the system was removed. Nitrite is accumulated in the middle process, and it is completely conver...

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Abstract

The invention belongs to the environmental biotechnical field and relates to a paracoccus denitrificans strain with a sulfur and ferrum co-substrate autotrophic denitrification function and a culturemethod and application thereof. The bacterium is Paracoccus denitrificans ZGL1. Under an anaerobic condition, nitrate or nitrite in waste water is reduced to nitrogen by taking iron pyrite or ferroussulfide as an electron donor. The bacterium is used for waste water denitrification through an autotrophic process of the sulfur and ferrum co-substrate. The energy consumption is low, the process issimple and the amount of sludge is small. The paracoccus denitrificans strain has a relatively good application potential in the field of waste water denitrification.

Description

technical field [0001] The invention belongs to the field of environmental biology technology, and relates to a paracoccus denitrification strain with autotrophic denitrification function of sulfur-iron co-substrate, a cultivation method and its application in the process of denitrification of wastewater. Background technique [0002] In recent years, with my country's industrial development and social progress, the discharge of industrial wastewater and domestic sewage has gradually increased. Although my country has adopted increasingly strict sewage treatment policies and measures, the problem of water pollution is still serious. According to the "China Environmental Status Bulletin" released in 2013, the discharge of ammonia nitrogen in industrial wastewater and municipal sewage has reached 2.536 million tons nationwide. In the visible period of time, the discharge of ammonia nitrogen pollutants in China is still quite huge. [0003] It is well known that when a large am...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC12N1/20C02F3/34C02F2101/163C02F2101/166C12R2001/01C12N1/205
Inventor 张玉葛小帅孙超越苏志强周翔孙利剑赵睿智周集体
Owner DALIAN UNIV OF TECH
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