Preparation method and application of sandwich type immunosensor for detecting stomach cancer tumor marker
A tumor marker and immunosensor technology, which is applied in the field of preparation of sandwich type immunosensors for detecting gastric cancer tumor markers, can solve the problems of cumbersome detection methods, high cost and high detection limit, and achieves good biocompatibility and low cost. , the effect of more active sites
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Embodiment 1
[0038] 1. A preparation method for detecting gastric cancer tumor marker sandwich type immunosensor, characterized in that the preparation steps are as follows:
[0039] (1) Preparation of h-MoS 2 / Au NPs
[0040] Take 20 mg of (NH 4 ) 2 MoS 4 and 2mL of 1% HAuCl 4 4H 2 O was mixed and dissolved in 20 mL of ultrapure water, and ultrasonicated for 10 min; then, 100 µL of hydrazine hydrate solution was added, and the mixed liquid was ultrasonically dispersed for 30 min to disperse the mixed liquid evenly; the mixed liquid was transferred to a 50 mL polytetrafluoroethylene reactor and heated to 180 ℃, reacted for 8 h; cooled to room temperature, and centrifuged and washed twice with ultrapure water and ethanol; the product was dissolved in 3 mL ultrapure water again, and dried in a lyophilizer to obtain h-MoS 2 / Au NPs;
[0041] (2) Preparation of Cu 2 O@TiO 2 / PtCu-Ab 2 Detection antibody incubation solution
[0042] Add 8 mL, 2.0 mol / L NaOH solution dropwise to 100 m...
Embodiment 2
[0060] 1. A preparation method for detecting gastric cancer tumor marker sandwich type immunosensor, characterized in that the preparation steps are as follows:
[0061] (1) Preparation of h-MoS 2 / Au NPs
[0062] Take 22 mg of (NH 4 ) 2 MoS 4 and 2mL of 1% HAuCl 4 4H 2 O was mixed and dissolved in 20 mL of ultrapure water, and ultrasonicated for 10 min; then, 100 µL of hydrazine hydrate solution was added, and the mixed liquid was ultrasonically dispersed for 30 min; the mixed liquid was transferred to a 50 mL polytetrafluoroethylene reactor , heated to 200 °C, and reacted for 10 h; cooled to room temperature, washed with ultrapure water and ethanol for 3 times; the product was dissolved in 3 mL ultrapure water again, and dried by a lyophilizer to obtain h-MoS 2 / Au NPs;
[0063] (2) Preparation of Cu 2 O@TiO 2 / PtCu-Ab 2 Detection antibody incubation solution
[0064] Add 10 mL, 2.0 mol / L NaOH solution dropwise to 100 mL, 0.01 mol / L CuCl containing 3.33 g polyvinyl...
Embodiment 3
[0082] 1. A preparation method for detecting gastric cancer tumor marker sandwich type immunosensor, characterized in that the preparation steps are as follows:
[0083] (1) Preparation of h-MoS 2 / Au NPs
[0084] Take 24 mg of (NH 4 ) 2 MoS 4 and 2mL of 1% HAuCl 4 4H 2 O was mixed and dissolved in 20 mL of ultrapure water, and sonicated for 10 min; then, 100 µL of hydrazine hydrate solution was added, and the mixture was sonicated for 30 min to disperse the mixture evenly; Heated to 220 °C and reacted for 12 hours; cooled to room temperature, centrifuged and washed four times with ultrapure water and ethanol; the product was dissolved in 3 mL ultrapure water again, and dried by a lyophilizer to obtain h-MoS 2 / Au NPs;
[0085] (2) Preparation of Cu 2 O@TiO 2 / PtCu-Ab 2 Detection antibody incubation solution
[0086] Add 12 mL, 2.0 mol / L NaOH solution dropwise to 100 mL, 0.01 mol / L CuCl containing 3.33 g polyvinylpyrrolidone 2 solution, fully stirred for 0.5 h; con...
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