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Test paper and kit for quickly screening late onset severepre-eclampsia

A technology for preeclampsia and late onset, applied in biological testing, material inspection products, measuring devices, etc., can solve problems such as non-occurrence, and achieve the effects of convenient use, rapid screening, and good clinical application prospects

Active Publication Date: 2018-11-09
邓成
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The main methods currently on the market can only screen patients for advanced preeclampsia by speculating on symptoms such as high blood pressure and proteinuria by experienced doctors. There is no qualitative and quantitative indicator to do so. Screening of patients with PE

Method used

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  • Test paper and kit for quickly screening late onset severepre-eclampsia
  • Test paper and kit for quickly screening late onset severepre-eclampsia
  • Test paper and kit for quickly screening late onset severepre-eclampsia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Comparison of APELA concentration in the blood of non-pregnant women, normal pregnant women, early PE pregnant women and late PE pregnant women

[0029] 1. Samples: 7 non-pregnant women, 34 normal pregnant women in the early pregnancy, 18 normal pregnant women in the second trimester, 15 normal pregnant women in the late pregnancy, 21 pregnant women with late PE, and 14 pregnant women with early PE; the above-mentioned pregnant women with late PE and early PE All pregnant women were diagnosed as late PE or early PE by chief obstetricians and gynecologists with more than 15 years of clinical experience, with high reliability.

[0030] 2. The detection method of APELA concentration in the blood of the subject:

[0031] (1) Serum extraction

[0032] Take 2-3mL of blood from the vein, without adding anticoagulant, place at room temperature for 30min, centrifuge at 2500rpm for 5min, transfer the serum to a new EP tube, and store at -80°C after aliquoting.

[0033...

Embodiment 2

[0064] Example 2 Contrast of APELA concentration in urine of non-pregnant women, normal pregnant women and late PE pregnant women

[0065] 1. Samples: 2 non-pregnant women, 7 normal pregnant women in the early pregnancy, 7 normal pregnant women in the second trimester, 8 normal pregnant women in the late pregnancy, and 4 pregnant women in the late PE; The chief physician of obstetrics and gynecology with clinical experience diagnosed late PE or early PE with high reliability.

[0066] 2. The detection method of APELA concentration in the subject's urine:

[0067] Fresh urine samples were centrifuged at 2500rpm at 4°C for 15-20min, and the supernatant was kept at -20 or -80.

[0068] APELA in urine was detected with Human ELA ELISA kit (Phoenix Pharmaceuticals, Inc. Cat. No. EK-007-19). Specific steps are as follows:

[0069] ① Add 50 μL of standard, urine sample and positive control to the 96 wells of the pre-coated secondary binding protein, and then add 25uL of primary bi...

Embodiment 3

[0085] Embodiment 3 The composition and method of use of the kit for detecting the concentration of APELA in the blood or urine of pregnant women according to the present invention

[0086] 1. The composition of the kit

[0087] Detection kit (50 persons)

[0088] components

volume

secondary binding protein

APELA standard

2500uL

primary binding protein

1250uL

Biotin-labeled peptides

1250uL

Assay Buffer

52500uL

DE SA-HRP solution

5000uL

Substrate solution

5000uL

Stop solution (2N HCl)

5000uL

[0089] 2. How to use the kit

[0090] (1) Serum extraction

[0091] Venous blood or urine 2-3mL, without anticoagulant, placed at room temperature for 30min, centrifuged at 2500rpm for 5min, then transferred serum or urine to a new EP tube, aliquoted and stored at -80°C.

[0092] (2) ELISA detection

[0093] Specific steps are as follows:

[0094] ① Add 50uL of standard, blood ...

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Abstract

The invention discloses test paper for quickly screening late onset severepre-eclampsia. The test paper for quickly screening late onset severepre-eclampsia comprises optional test paper for detectingthe APELA concentration in blood or urine. The invention also discloses a kit for screening late onset severepre-eclampsia. The kit comprises an optional reagent for detecting the APELA concentrationin blood or urine. The invention also discloses application of the test paper for detecting the APELA concentration in blood or urine in preparing the test paper for screening late onset severepre-eclampsia. The invention also discloses application of the reagent for detecting the APELA concentration in blood or urine in preparing a reagent for screening late onset severepre-eclampsia. Accordingto the test paper and the kit for quickly screening late onset severepre-eclampsia disclosed by the invention, only blood and urine of a patient need to be acquired, and whether the patient is suffered from late onset severepre-eclampsia or not can be screened, so that the test paper and the kit can be used for noninvasively, effectively and quickly examining and screening late onset severepre-eclampsia, and are convenient to use and favorable in clinic application prospect.

Description

technical field [0001] The invention relates to a test paper and a kit for rapid screening of late-onset preeclampsia. Background technique [0002] Preeclampsia (preeclampsia) belongs to the category of hypertensive disorders of pregnancy and is a multisystem disease that is idiopathic during pregnancy. Preeclampsia manifests as high blood pressure and proteinuria in pregnant women, with or without multiple organ damage, fetal growth restriction, fetal distress, and oligohydramnios, which seriously threaten the life of the mother and fetus. The incidence of preeclampsia is about 5% to 8%. According to the gestational age of occurrence, it is divided into early onset preeclampsia and late onset preeclampsia. 34 weeks of gestation is the boundary line between early onset and late onset. Preeclampsia before 34 weeks is called early-onset preeclampsia and late-onset preeclampsia after 34 weeks of gestation. Among them, late-onset preeclampsia accounts for the vast majority of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558
CPCG01N33/558G01N33/6893G01N2800/368
Inventor 邓成
Owner 邓成
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