A method for recovering sweet tea polyphenols from sweet tea flocculation slag after extracting rubusoside

A technology for sweet tea polyphenols and sweet tea glycosides, applied in the direction of organic chemistry and the like, can solve the problems such as the content of polyphenols cannot reach 90%, the pretreatment process of sweet tea leaves is complicated, the waste of sweet tea polyphenol resources, etc., and achieves low cost and short extraction time. , the effect of reducing pollution

Active Publication Date: 2020-10-02
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is: the pretreatment process of sweet tea leaves is more complicated, and the extract is directly passed through the D-101 macroporous adsorption resin, rubusoside and sweet tea polyphenols will be adsorbed at the same time, and most of the sweet tea polyphenols will be adsorbed on the resin , the amount of sweet tea polyphenols in the effluent is less, so a large amount of resources of sweet tea polyphenols will be wasted
Although this method can obtain high-purity sweet tea polyphenols, the cost of alcohol extraction is high, and when using macroporous adsorption resin to absorb sweet tea polyphenols, it will also enrich rubusoside, so the polyphenol content cannot reach more than 90%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

reference example 1

[0060] The macroporous adsorbent resins adopted in the reference examples or examples of the present invention are all soaked in 2BV ethanol solution with a volume concentration of 95% for 24 hours before use, and then washed with an ethanol solution with a volume concentration of 95% until the effluent is colorless and odorless. Wash with deionized water until there is no alcohol smell, then use 3BV of 5% sodium hydroxide solution for alkali washing, the flow rate is 1BV / h, then wash with water until neutral, and finally pickle with 3BV of 5% hydrochloric acid solution, The flow rate is 1BV / h, and then washed with water until neutral.

reference example 2

[0062] Before using the alumina used in the examples of the present invention, first activate the alumina at a high temperature of 550°C for 18 hours, and then wash it with an ethanol solution with a volume fraction of 95% until the effluent is colorless and transparent, and the smell of ethanol is removed. Until there is no other smell outside, finally wash with water until there is no ethanol smell.

reference example 3

[0064] Crush 1000kg of sweet tea dry leaves, pass through a 20-mesh sieve, and extract 3 times under reflux at 90°C (add 10t of water for the first time, 8t for the second time, and 6t for the third time), extract for 2 hours each time, and extract at a speed of 20000r / min, butterfly centrifugation to obtain 21t of extract; add 21kg FeCl to 21t of extract 3 , at a pH value of 4, fully stir for 30 minutes, let stand for 50 minutes, then adjust the pH value to 9 with saturated lime water, stir for 30 minutes, let stand for 50 minutes for flocculation, and use a plate filter with a filter membrane pore size of 1.0 μm for plate Frame pressure filtration, and wash the flocculation residue until it has no sweet taste, collect 2.3t of sweet tea flocculation residue 1 after extracting rubusoside (water content 70.5%, sweet tea polyphenols mass content 4.35%), and the flocculation liquid at a flow rate of 0.5BV / h , after applying D-101 macroporous adsorption resin, wash with water at ...

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PUM

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Abstract

A method for recovering sweet tea polyphenols from sweet tea flocculation slag after rubusoside extraction, comprising the following steps: (1) acid dissolution: adding an acid solution to the sweet tea flocculation slag after extraction of rubusoside, stirring until the flocculation slag dissolves, Centrifuge and filter to obtain filtrate; (2) Macroporous adsorption resin enrichment: enrich the filtrate on a macroporous adsorption resin column, wash with water, elute with organic solvent, concentrate the eluate under reduced pressure and recover the solvent to obtain a concentrated solution; (3) Alumina refining: put the concentrated solution on the alumina chromatography column, wash with water, gradient elution, concentrate the eluate under reduced pressure and recover the eluent, dry in vacuum at low temperature to obtain sweet tea polyphenol products. The purity of the sweet tea polyphenol product obtained by the method of the present invention is ≥96%, and the yield is ≥90%. Not only wastes are reused, cost is saved, but also environmental pollution is reduced; the method of the present invention has short extraction time, simple operation process, energy consumption, The cost is low, and continuous mass production can be realized.

Description

technical field [0001] The invention relates to a method for recovering sweet tea polyphenols, in particular to a method for recovering sweet tea polyphenols from sweet tea flocculation slag after extracting rubusoside. Background technique [0002] Rubus is a perennial deciduous shrub belonging to the family Rosaceae, Rubus. Studies have shown that sweet tea polyphenols have good biological activity, anti-inflammatory, anti-oxidation, anti-allergic and anti-aging effects, and have a good application prospect. Usually, in the process of rubusoside production, sweet tea polyphenols are discharged as waste. On the one hand, it causes a huge waste of resources and also pollutes the environment. Even if it is recycled, the cost is high and the product purity is low. [0003] CN105012359A discloses a method for recovering sweet tea polyphenols. The sweet tea leaves are cleaned, greened, quick-frozen, microwave-thawed 3 times, steam-exploded, extracted with an organic solvent, tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
CPCC07G99/00
Inventor 陈钱龙伟岸黄华学贺进军
Owner HUNAN HUACHENG BIOTECH
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