Rana nigromaculata spawn incubation method
A technology for frog eggs and black spots, which is applied in the field of breeding, can solve the problems of easily breaking the egg membrane of frog eggs, low number and quality of frogs, and low hatching rate of frog eggs, so as to ensure hatching synchronization, suitable pH, and avoid eggs. The effect of membrane rupture
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Embodiment 1
[0017] A method for hatching the frog eggs of the black-spotted frog, comprising adding the extract solution of the jellygrass jelly to water in a mass ratio of 1:20, and then adding it to a hatching pond, and then putting the frog eggs of the black-spotted frog into the hatching pond for hatching at 20°C.
[0018] Further, the extract of the jelly grass accounts for three-quarters of the volume of the hatching pond after being watered.
[0019] Further, the jelly jelly extract is obtained by a microwave-assisted lye extraction method.
[0020] Further, the preparation method of the Herba jelly extract is:
[0021] Mix jelly jelly and aloe vera in a mass ratio of 5:1.2, pulverize, pass through a 100-mesh sieve to obtain a powder, add 12 times its mass of clear water to the powder, and stir for 20 minutes at a speed of 1200r / min to obtain a mixed slurry, and then use Adjust the pH of the mixed slurry to 8.9 with sodium carbonate, heat to 60°C, extract under 350W microwave for ...
Embodiment 2
[0027] A method for hatching the eggs of the black-spotted frog, adding water to the extract solution of the black-spotted frog in the hatching tank after adding the extract solution of the jellyfish at a mass ratio of 1:20, and then putting the frog eggs of the black-spotted frog in the hatching tank for hatching at 22°C.
[0028] Further, the extract of the jelly grass accounts for three-quarters of the volume of the hatching pond after being watered.
[0029] Further, the jelly jelly extract is obtained by a microwave-assisted lye extraction method.
[0030] Further, the preparation method of the Herba jelly extract is:
[0031] Mix jelly jelly and aloe vera in a mass ratio of 5:1.5, pulverize, pass through a 100-mesh sieve to obtain a powder, add 15 times its mass of clear water to the powder, and stir for 20 minutes at a speed of 1200r / min to obtain a mixed slurry, and then use Adjust the pH of the mixed slurry to 8.9 with sodium carbonate, heat to 65°C, extract under 35...
Embodiment 3
[0037] A method for incubating the eggs of the black-spotted frog, comprising adding the extract solution of Herba japonicus to water in a mass ratio of 1:20, and then adding it to a hatching pond, and then putting the frog eggs of the black-spotted frog into the hatching pond for hatching at 21°C.
[0038] Further, the extract of the jelly grass accounts for three-quarters of the volume of the hatching pond after being watered.
[0039] Further, the jelly jelly extract is obtained by a microwave-assisted lye extraction method.
[0040] Further, the preparation method of the Herba jelly extract is:
[0041] After mixing jelly jelly and aloe vera in a mass ratio of 5:1.3, pulverize and cross a 100-mesh sieve to obtain a powder, add 13 times its mass of clear water to the powder, and stir for 20 minutes at a speed of 1200r / min to obtain a mixed slurry, and then use Adjust the pH of the mixed slurry to 8.9 with sodium carbonate, heat to 62°C, extract under 350W microwave for 25 ...
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