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Lactic-acid-bacterium sports beverage capable of enhancing immunity and preparation method thereof

A technology for enhancing immunity and lactic acid bacteria beverage is applied in the field of sports lactic acid bacteria beverage and its preparation, which can solve problems such as the influence of immunity on digestion and absorption capacity of the gastrointestinal tract, and achieve the effects of good antimicrobial effect, easy process control and simple method.

Active Publication Date: 2018-11-02
JUNLEBAO DAIRY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to the massive consumption of nutrients such as electrolytes, sugar, protein, and vitamins before and after exercise, when the body is stressed before exercise and fatigued during or after exercise, immunity and the digestion and absorption capacity of the gastrointestinal tract may be greatly affected

Method used

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  • Lactic-acid-bacterium sports beverage capable of enhancing immunity and preparation method thereof
  • Lactic-acid-bacterium sports beverage capable of enhancing immunity and preparation method thereof
  • Lactic-acid-bacterium sports beverage capable of enhancing immunity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 A sports lactic acid bacteria drink with enhanced immunity and its preparation method

[0036] The present embodiment is a sports-type lactic acid bacteria drink with enhanced immunity, which is carried out in sequence according to the following steps:

[0037] (11) Preparation of whey fermentation broth

[0038] (1Ⅰ) Take 15kg of whey powder, 1kg of sucrose and 10kg of water, mix them well, and keep them at 65℃ for 30min to obtain A1;

[0039] (1II) Cool A1 to 40 °C, inoculate Lactobacillus deutschmus bulgaricus 0.0001 kg, Streptococcus thermophilus JMCC0019 0.0001 kg, Lactobacillus plantarum N3117 0.0001 kg, Lactobacillus paracasei N1115 0.0001 kg, and ferment at 37 °C 8h, stop the fermentation when the pH is 4.6 to obtain B1;

[0040] (1Ⅲ) Stir B1 evenly, after-cooking at 4°C for 4 hours, to obtain whey fermentation broth, for use;

[0041] (12) Preparation of sports lactic acid bacteria drink with enhanced immunity

[0042] (11) Homogenize 15 kg of ...

Embodiment 2-5

[0045] Example 2-5 Sports lactic acid bacteria drink with enhanced immunity and preparation method thereof

[0046] The present embodiment is respectively a sports lactic acid bacteria drink with enhanced immunity, and the preparation method is similar to that of Example 1, the difference is only that the corresponding parameters in the preparation process are different, as shown in the following table:

[0047]

[0048] The beverage prepared in this embodiment is rich in nutrition, good in taste, unique in flavor and good in anti-microbial effect, and has the function of regulating the resistance of exercising human body.

Embodiment 6

[0049] Example 6 Evaluation of in vitro cell model

[0050] In this example, different addition amounts of Lactobacillus plantarum N3117 and Lactobacillus paracasei N1115 were set, and the predominant ratios of the above two raw materials were evaluated through an in vitro cell model. The specific evaluation methods are as follows:

[0051] The cultured Caco-2 and J774A.1 cells were pre-treated, washed twice with sterile PBS, and then added 1 mL of antibiotic-free DMEM culture medium and heat-killed Lactobacillus plantarum N3117 and paracheese milk with different contents. Bacillus N1115 lyophilized powder, 5% CO 2 Incubator at 37 °C for 3 h. After standing, washed three times with sterile PBS, extracted the total RNA of Caco-2 and J774A.1 cells, transcribed into cDNA and used to detect interleukin-6 (Interleukin-6, IL-6), interleukin -12 (Interleukin-12, IL-12) and tumor necrosis factor-α (Tumor necrosis factor-α, TNF-α) expression. Real-time PCR was used for detection. T...

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Abstract

The invention discloses a lactic-acid-bacterium sports beverage capable of enhancing immunity. The lactic-acid-bacterium sports beverage capable of enhancing immunity comprises the following raw materials in parts by weight: 15-30 parts of malt-oligosaccharides, 15-25 parts of glucose, 10-20 parts of whey fermentation liquid, 0.1-1 part of KCl, 0.1-5 parts of NaCl, 0.5-5 parts of citric acid, 0.2-2 parts of vitamins and 60-85 parts of water. The lactic-acid-bacterium sports beverage is rationally matched, as well as rich in nutrients and good in taste. The whey fermentation liquid is preparedby adopting four kinds of strains, so that, lactic acid is produced by lactose in whey in result of fermentation of abundant nutrients in the whey by using the lactic acid bacteria. The produced lactic acid is used as an acidulant for developing beverages, and has unique flavor as well as good anti-microorganism effects. The invention further discloses a preparation method of the lactic-acid-bacterium sports beverage capable of enhancing immunity. The preparation method of the lactic-acid-bacterium sports beverage capable of enhancing immunity comprises the steps of uniformly mixing raw materials, and carrying out sterilization. Thus, the preparation method is simple, easy in process control, short in production cycle and low in cost. The preparation method is suitable for preparing a lactic-acid-bacterium sports beverage capable of enhancing immunity.

Description

technical field [0001] The invention belongs to the field of food, and relates to the preparation of a lactic acid bacteria beverage, in particular to a sports lactic acid bacteria beverage with enhanced immunity and a preparation method thereof. Background technique [0002] With the general improvement of people's living standards in our country and the implementation of the national fitness program, in recent years, endurance sports such as brisk walking and marathon have become a new fashion that leads the national fitness in China. However, during long-term or high-intensity exercise, the energy consumption of the human body continues to increase and symptoms such as muscle soreness and mental fatigue appear, which enhance the body's material and energy metabolism activities, and increase the consumption of water, electrolytes and energy substances. Supplement in a timely manner. Functional sports drinks are a type of fast replenishment products based on the purpose of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C21/02A23C21/08A23C21/10
CPCA23C21/026A23C21/08A23C21/10A23V2400/137A23V2400/165A23V2400/169A23V2400/249
Inventor 荀一萍朱宏王世杰王璐冯丽莉张栋薛玉玲
Owner JUNLEBAO DAIRY GRP CO LTD
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