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Method for Establishing Fingerprint of Angelica Decoction Pieces and Its Fingerprint

A technology of fingerprints and decoction pieces, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of limited sample size, low comparability, and non-consideration, so as to avoid singleness and one-sidedness, large number of peaks, and baseline smooth effect

Inactive Publication Date: 2020-12-22
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE +1
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  • Application Information

AI Technical Summary

Problems solved by technology

Although the predecessors have reported the HPLC fingerprints of the medicinal materials of Minxian Angelica sinensis, the sample size is limited, and the quality comparison between the medicinal materials of Angelica sinensis and the commercially available decoction pieces has not been considered.

Method used

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  • Method for Establishing Fingerprint of Angelica Decoction Pieces and Its Fingerprint
  • Method for Establishing Fingerprint of Angelica Decoction Pieces and Its Fingerprint
  • Method for Establishing Fingerprint of Angelica Decoction Pieces and Its Fingerprint

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preparation example Construction

[0039] 2.1 Selection of preparation conditions for the test solution

[0040] 2.1.1 Selection of extraction solvent

[0041] Accurately weigh 0.5g of 3 parts of No. 14 angelica decoction pieces and place them in a 50mL conical flask with a stopper, and add 25mL of 25% methanol, 50% methanol, 70% methanol, 5% formic acid methanol, and 100% acetonitrile solution as extraction Agent, ultrasonic treatment (power 250W, frequency 40kHz) 30min, take out, let cool, make up for weight loss, shake up, get the continued filtrate, obtain the need testing solution. The fingerprints were measured under the same detection conditions ( figure 1 -A, 1-B, 1-C, 1-D, 1-E), determine the best extraction solvent by comparison, such as figure 1. Under the same conditions of the remaining detection methods, it was found by comparison that when the extraction solvent was 25% methanol and 100% acetonitrile, the chromatogram peak response of the obtained test product chromatogram was lower and the nu...

Embodiment 1

[0053] Establishment of fingerprints of Angelica decoction pieces

[0054] (1) Preparation of the test solution: Take about 0.5 g of angelica decoction pieces powder (passed through a No. 3 sieve), accurately weigh it, put it in a 50 mL conical flask with a stopper, add 25 mL of 5% methanol formic acid, weigh it, and ultrasonically Treat (power 250W, frequency 40kHz) for 30min, take it out, let it cool, make up for weight loss, shake well, take the continued filtrate as the test solution;

[0055] (2) Preparation of reference solution: Weigh an appropriate amount of ferulic acid, coniferyl ferulic acid, ligustilide I, ligustilide H, ligustilide, n-butylphthalide, butenylphthalide The reference substance, accurately weighed, was prepared by adding acetonitrile with mass concentrations of 0.06mg / mL, 0.19mg / mL, 0.12mg / mL, 0.52mg / mL, 0.87mg / mL, 0.28mg / mL, 0.61mg / mL Mix the reference substance solution, pass through a 0.22 μm microporous membrane, and take the subsequent filtrate ...

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Abstract

The invention discloses a method for establishing a Chinese angelica decoction piece fingerprint as well as the fingerprint, and relates to the technical field of a fingerprint of traditional Chinesemedicine materials. The method comprises preparation of a test sample solution and a high-performance liquid chromatography detection condition, atlas is processed by using fingerprint software, and the fingerprint of the Chinese angelica decoction piece is acquired; chromatographic data is imported into SPSS19.0 version software to perform clustering analysis, and the clustering result of a standard self-made decoction piece and a commercially available detection piece is obtained. The method has the following positive effects: the mutual mode of the Chinese angelica decoction piece HPLC fingerprint is established, 29 common peaks are calibrated, the quality can be effectively represented, the simplicity of the original quality control method is overcome, and the application value is relatively high.

Description

technical field [0001] The invention relates to the technical field of fingerprints of Chinese medicinal materials, in particular to a method for establishing fingerprints of angelica decoction pieces and the fingerprints thereof. Background technique [0002] Angelica sinensis (Oliv.) Diels is the dry root of the perennial Angelica sinensis of Umbelliferae, also known as Xigui, Qingui, and Yungui. It was first recorded in "Shen Nong's Materia Medica". county. Its raw product is warm in nature, sweet and pungent in taste, and returns to the liver, heart and spleen meridian. , is a traditional Chinese medicine commonly used in clinical practice, and there is a record in "Compendium of Materia Medica" by Li Shizhen in the Ming Dynasty that "Angelica regulates blood and is a medicine for women". According to literature reports, angelica mainly contains phenylpeptides, organic acids and their esters, sugars and other components, which have various pharmacological effects such ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 包贝华石海培张丽严辉黄胜良汪国强左武鹏
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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