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A method for preparing six compounds of oxytroflavoside from Oxytropis falciparum and its application

A technology of Oxytropis falciparum and crude extracts, which is applied in organic chemistry, pharmaceutical formulations, drug combinations, etc., can solve the problems of complex and cumbersome extraction methods, poor repeatability, environmental impact, etc., and achieve broad market application prospects, simple operation, The effect of high purity

Active Publication Date: 2021-03-23
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Wang et al. (Wang S S, Zhang X J, Que S, et al.3-Hydroxy-3-methylglutaryllavonol glycosides from Oxytropis falcata.[J].Journal of Natural Products,2012,75(7):1359-1364) for the first time from Seven kinds of flavonol derivatives were extracted from Oxytropis falciparum: Oxytroflavoside A, B, C, D, E, F, G. However, the extraction method is complicated and cumbersome, and the separation method adopts the conventional silica gel column chromatography method, which takes a long time , poor repeatability, and has a great impact on the environment

Method used

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  • A method for preparing six compounds of oxytroflavoside from Oxytropis falciparum and its application
  • A method for preparing six compounds of oxytroflavoside from Oxytropis falciparum and its application
  • A method for preparing six compounds of oxytroflavoside from Oxytropis falciparum and its application

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Experimental program
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Effect test

Embodiment 1

[0028] The preparation of embodiment 1, Oxytroflavoside A, B, C, D, F, G

[0029] (1) Extraction: Take 2 kg of Oxytropis falciparum and grind it, extract with 60 L of 5% V / V ethanol solution to obtain an extract, and recover the ethanol to obtain a crude extract.

[0030] (2) Impurity removal:

[0031] a Take the above crude extract, filter it with a 0.1μm-0.5μm ceramic membrane (pressure: 3MPa, flow rate 50-500ml / min) to remove fine slag, soil and other impurities;

[0032] b Take the extract obtained in step a, wash it successively with petroleum ether and ethyl acetate, absorb the washed water phase with AB-8 macroporous resin, wash the resin column with water, first elute with 40% ethanol solution to remove impurities, and then Elute with 60% ethanol solution, collect the 60% ethanol eluate, and recover the ethanol to obtain the crude extract.

[0033] (3) One-dimensional separation and preparation: take the crude extract obtained in step (2), and use C18 chromatographic...

Embodiment 2

[0040] The preparation of embodiment 2, Oxytroflavoside A, B, C, D, F, G

[0041] (1) Extraction: Take 2 kg of Oxytropis falciparum and grind it, extract with 10 L of 95% V / V ethanol solution to obtain an extract, and recover the ethanol to obtain the extract.

[0042] (2) Impurity removal:

[0043] a Take the above-mentioned extract, add water to dissolve it, filter it with a ceramic membrane of 0.1 μm ~ 0.5 μm (pressure: 3 MPa, flow rate 50 ~ 500ml / min) to remove fine slag, soil and other impurities;

[0044] b Take the extract obtained in step a, wash it successively with petroleum ether and ethyl acetate, absorb the washed water phase with D101 macroporous resin, wash the resin column with water, first elute and remove impurities with 40% ethanol solution, and then use 60 % ethanol solution for elution, collect 60% ethanol eluate, and recover the ethanol to obtain the crude extract.

[0045] (3) One-dimensional separation and preparation: take the crude extract obtained ...

Embodiment 3

[0049] Embodiment 3, the preparation of Oxytroflavoside A, B, C, D, F, G

[0050] (1) Extraction: Take 2kg of Oxytropis falciparum and crush it, extract it with 40L of 50% V / V ethanol solution to obtain the extract, recycle the ethanol to obtain the extract.

[0051] (2) Impurity removal:

[0052] a Take the above-mentioned extract, add water to dissolve it, filter it with a ceramic membrane of 0.1 μm ~ 0.5 μm (pressure: 3 MPa, flow rate 50 ~ 500ml / min) to remove fine slag, soil and other impurities;

[0053] b Take the extract obtained in step a, wash it successively with petroleum ether and ethyl acetate, absorb the washed water phase with D101 macroporous resin, wash the resin column with water, first elute and remove impurities with 40% ethanol solution, and then use 60 % ethanol solution for elution, collect 60% ethanol eluate, and recover the ethanol to obtain the crude extract.

[0054] (3) One-dimensional separation and preparation: take the crude extract obtained in...

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Abstract

The invention provides a method for preparing oxytroflavoside A, B, C, D, E, F and G from oxytropis falcate bunge. The method comprises the following steps: (1) extraction: pulverizing the oxytropis falcate bunge, extracting the oxytropis falcate bunge with ethanol to obtain an extracting solution, and recycling the ethanol to obtain a crude extracting solution; (2) impurity removal: filtering thecrude extracting solution with a ceramic film and then separating the crude extracting solution with macroporous resin columns, carrying out elution by using a 40% ethanol solution and a 60% ethanolsolution successively, collecting 60% ethanol eluant, and recycling ethanol to obtain a crude extract; (3) one-dimensional separation and preparation: carrying out gradient elution on the crude extract obtained from step (2) by using a C18 chromatographic column to obtain four components including Fr.1, Fr.2, Fr.3 and Fr.4; and (4) two-dimensional separation and preparation: separately eluting thefour components obtained from step (3) by using C18 chromatographic columns to obtain the oxytroflavoside A, B, C, D, E, F and G. The method is simple and convenient to operate, the purity of the obtained product is high, and the market application prospect is wide.

Description

technical field [0001] The invention relates to a method for preparing six compounds of oxytroflavoside from Oxytropis falciparum and its application. Background technique [0002] Oxytropis falcate Bunge is a medicinal plant belonging to the genus Oxytropis of the Fabaceae family. It is a perennial herb and is widely distributed in beaches and sandy lands at an altitude of 2700-4300m in Tibet, Qinghai, southern Gansu, western Sichuan and Xinjiang. Mountains, slopes, valleys, and meadows are rich in resources. Tibetan medicine is called "E Daxia" and "Daxia". One of the folk herbs. [0003] Oxytropis falciparum mainly contains alkaloids, flavonoids and other compounds, among which flavonoids have antibacterial, anti-oxidative free radical, anti-tumor, and cough-relieving activities. At present, more than 20 kinds of flavonoids have been isolated, mainly in the form of flavone glycosides, flavones and dihydroflavones, flavonols, chalcones, hydrochalcones, isoflavanes and fl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07A61K31/7048A61P29/00
CPCA61K31/7048A61P29/00C07H17/07
Inventor 陈晨李玉林陈涛谭亮王环
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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