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Application of fvcpc2 protein and its coding gene in regulating mycelial growth and fruiting body development of various edible fungi

A technology for mycelial growth and encoding genes, applied in the field of genetic engineering, can solve problems such as lack of universal applicability

Active Publication Date: 2021-03-30
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These studies are currently limited to the study of a single species and do not have general applicability among different edible fungi

Method used

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  • Application of fvcpc2 protein and its coding gene in regulating mycelial growth and fruiting body development of various edible fungi
  • Application of fvcpc2 protein and its coding gene in regulating mycelial growth and fruiting body development of various edible fungi
  • Application of fvcpc2 protein and its coding gene in regulating mycelial growth and fruiting body development of various edible fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] Embodiment 1, the construction of Fvcpc2 overexpression mutant strain and Fvcpc2 knockdown expression mutant strain

[0119] 1. Nucleotide sequence of Fvcpc2 gene, amino acid sequence of FvCPC2 protein and expression level of Fvcpc2 in different periods

[0120] 1. Nucleotide sequence of Fvcpc2 gene and amino acid sequence of FvCPC2 protein

[0121] The present invention is effective in treating multiple filamentous fungi (comprising Neurospora crassa, Aspergillus, Fusarium, Schizophyllum and Coprinus cinerea) and multiple edible fungi (comprising Pleurotus bisporus, Ganoderma lucidum, shiitake mushroom, Flammulina velutipes and grass Mushroom) genome and expression profile data analysis process during fruiting body development, a common conserved functional protein coding gene from Flammulina velutipes was obtained, and it was named Fvcpc2. There is no report on its function. The Fvcpc2 gene of the present invention has a full length of 1273bp from the start codon to...

Embodiment 2

[0226] Embodiment 2, the application of Fvcpc2 in regulating the mycelial growth of Flammulina velutipes

[0227] 1. Observation experiment of mycelial growth on plate for Fvcpc2 mutant strain

[0228] Wild type Flammulina velutipes, Fvcpc2 overexpression mutant strain (Fvcpc2 OE#5 , Fvcpc2 OE#33 and Fvcpc2 OE#124 ) and Fvcpc2 knockdown expression mutant strains (Fvcpc2 RNAi#11 , Fvcpc2 RNAi#41 and Fvcpc2 RNAi#43 ) were respectively made into bacterial blocks (d=5mm) of the same size with a puncher, and were inoculated on drug-free CYM plates, cultured at 25°C for 7 days and photographed. During the cultivation process, the growth length of mycelium was measured every 24 hours, and recorded, and the average growth rate of each bacterial strain was calculated.

[0229] The results of the analysis of the growth and growth rate of different strains are as follows: On the CYM plate, the growth of mycelia of the Fvcpc2 overexpression mutant strain was not significantly differ...

Embodiment 3

[0233] Embodiment 3, the application of Fvcpc2 in regulating the development of the fruiting body of Flammulina velutipes

[0234] 1. Observation experiment on fruiting of Fvcpc2 mutant strain

[0235] 1. Wild type Flammulina velutipes, Fvcpc2 overexpression mutant strain (Fvcpc2 OE#5 , Fvcpc2 OE#33 and Fvcpc2 OE #124 ) and Fvcpc2 knockdown expression mutant strains (Fvcpc2 RNAi#11 , Fvcpc2 RNAi#41 and Fvcpc2 RNAi#43) were respectively made into bacterial blocks (d=5mm) of the same size with a puncher, and were respectively inoculated into tissue culture bottles equipped with 325g cultivation material, cultivated at 25°C, 70% humidity, and cultivated in the dark for 15-20 days until All strains mycelium overgrown the cultivation bottle.

[0236] 2. Scratch each cultivation bottle separately, that is, gently scrape off the thick mycelia on the top of the cultivation bottle with a sterilized scalpel, and continue to cultivate at 25°C, 70% humidity, and dark conditions unt...

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Abstract

The invention discloses an application of FvCPC2 protein and a coding gene thereof in regulating and controlling the mycelial growth and fruiting body development of various edible fungi. The overexpression and knockdown expression experiments of FvCPC2 in flammulina velutipes show that the FvCPC2 influences the growth of the flammulina velutipes mycelia in the flat plate and the culture material,and determines the formation of the flammulina velutipes primordium and the development of the fruiting body, and the FvCPC2 can be applied to the breeding of the excellent strain of the flammulina velutipes. At the same time, the FvCPC2 can replace the control function of cpc-2 mycelial growth and fruiting body development in the neurospora crassa through the replenishment experiment, which indicates that FvCPC2 is not only ubiquitous in basidiomycetes and ascomycetes, but also has the universality of the regulation and control function of fruiting body development and can be applied to thebreeding of various edible fungi. The FvCPC2 is the first discovery in the edible fungi, and is also the first application in the genetic engineering breeding of the edible fungi.

Description

technical field [0001] The present invention relates to the technical field of genetic engineering, in particular to the application of FvCPC2 protein in regulating the mycelial growth and fruiting body development of various edible fungi (including Shiji mushroom, Agaricus bisporus, Ganoderma lucidum, Lentinus edodes, Flammulina velutipes and Straw mushroom), and a strain with Fvcpc2 overexpression mutant strain of Flammulina velutipes (Fvcpc2 OE#5 ). Background technique [0002] Edible fungi refer to fungi that can form large fruiting bodies or sclerotium-like tissues, and can be used for human consumption or medicine (Zhang Weidong et al., Gardening Specialty Products, 2017). At present, there are more than 1,000 edible fungi in my country (Dai Yucheng et al., Acta Mycophyta Sinica, 2010.29: p.625-628.), most of them belong to Basidiomycetes, and a small part belong to Ascomycetes. Edible fungi are rich in polysaccharides, proteins, triterpenoids, polypeptides, dietary ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/375C12N1/15C12N15/80C12R1/645
CPCC07K14/375C12N15/80
Inventor 李少杰吴塔菊孙宪昀张振颖
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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