Anti-ageing and wrinkle-removing composition containing dendrobium stem cell extract and application thereof
An extract and anti-aging technology, applied in the field of anti-aging and wrinkle-removing compositions containing Dendrobium stem cell extract, to achieve the effect of improving skin elasticity, strong anti-aging activity and improving skin quality
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[0032] The preparation method of the dendrobium stem cell extract of the present invention is as follows: 1) select high-quality dendrobium leaves, clean and disinfect, and cut into powder to make callus; 2) inoculate the callus on a solid medium for induction Cultivate, cut the regenerated part of the callus for subculture, transfer the cultured product to a liquid medium for expanded culture, and then transfer to a bioreactor for large-scale culture until a saturated dendrobium stem cell suspension is obtained; 3 ) Filter the Dendrobium stem cell suspension obtained in 2) with a 1 μm filter, remove the culture medium, and harvest the retained cells; put the cells in ultra-low temperature freezing at -80°C for 2 hours, then remove and thaw for 30 minutes, and repeat the above operation for 5 minutes After the last time of thawing at room temperature, the cells were broken with an ultrasonic breaker, filtered through a filter, and the filtrate was collected and concentrated in ...
Embodiment 1
[0042] The anti-aging wrinkle-removing composition described in this embodiment includes the following ingredients in parts by weight: 1.5 parts of Dendrobium stem cell extract, 10 parts of Rhodiola root extract, 5 parts of purslane extract, and 5 parts of Panax notoginseng extract .
[0043] The composition of this embodiment can be obtained by mixing the stem cell extract of dendrobium, the root extract of Rhodiola rosea, the extract of purslane and the extract of notoginseng according to the parts by weight.
[0044] This embodiment provides that the anti-aging and wrinkle-removing composition is applied in skin care products, and the skin care products include the following ingredients in mass percentage: 5.0% of anti-aging and wrinkle-removing composition, 10% of glycerin, 0.2% of sodium hyaluronate, polydimethylformaldehyde 2% silicone, 3% glyceryl stearate, 1% xanthan gum, 0.3% phenoxyethanol, and the balance is deionized water.
[0045] The preparation method of above...
Embodiment 2
[0049] The anti-aging wrinkle-removing composition described in this embodiment includes the following components by weight: 2 parts of stem cell extract of Dendrobium, 12 parts of rhodiola root extract, 6 parts of purslane extract, 4 parts of Panax notoginseng extract .
[0050] This embodiment provides that the anti-aging composition is applied in skin care products, and the skin care products include the following components in mass percentage: 4% of anti-aging composition, 10% of glycerin, 1% of potassium cetyl phosphate, 3% of jojoba oil %, acacia gum 1.5%, phenoxyethanol 0.5%, and the balance is deionized water.
[0051] The preparation method of above-mentioned skin care product, comprises the following steps:
[0052] 1) Add glycerin and acacia gum to the emulsification pot, then add deionized water, stir at room temperature until it is completely dispersed, then heat up to 75-80°C while stirring, continue stirring until it dissolves evenly, and keep the temperature; ...
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