Method for constructing animal model of the rabbit knee cartilage subchondral bone injury and method for evaluating osteogenic material

Abstract

The invention discloses a method for constructing animal model of the rabbit knee cartilage subchondral bone injury and a method for evaluating osteogenic material. The method for constructing animalmodel of the rabbit knee cartilage subchondral bone injury comprises the following steps of: (1) carrying out general anesthesia treatment on the test New Zealand rabbits and fixing in supine position; (2) carrying out skin preparation on one side knee joint of the New Zealand rabbit; (3)carrying out the longitudinal incision by using the knee joint as the center, cutting open along the gap of themedial side of the patella and the quadriceps femoris, and turning the patella to expose the joint cavity and the articular surface of the tibial plateau,; (4) longitudinally forming a cylindrical hole in the median longitudinal direction of the joint surface of the tibial plateau by using a corneal ring drill so as to obtain an animal model of the rabbit knee cartilage subchondral bone injury. The rabbit knee cartilage subchondral bone injury animal model can effectively evaluate the osteogenic drug or the osteogenic material, thereby providing a reliable basis for screening and evaluating the osteogenic drug or the osteogenic material.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular, the invention relates to a method for constructing an animal model of rabbit knee subchondral bone injury and a method for evaluating osteogenic materials. Background technique [0002] Knee Osteoarthritis (KOA) animal models are diverse, including animal sources, and production methods. The reproducibility of animal models is crucial for elucidating the mechanism of OA disease, and it is also more helpful to apply the experimental results as soon as possible. clinical. Among the commonly used OA models, in addition to different animal choices (mice, rats, guinea pigs, rabbits, dogs, goats, horses, etc.), the preparation methods also have their own characteristics. The main methods are: 1. Spontaneous formation, commonly used animals include : Rodents, dogs, horses, such as: C57 / BI6, Balb / c mice, treadmill-trained rats, guinea pigs, etc. Evaluation: Clinically relevant, but the disease ...

AI Technical Summary

Problems solved by technology

Disadvantages: slow disease progression, moderate cartilage damage, highly clinically relevant, no joint instability, trauma modeling required

However, the existing animal models involving knee subchondral bone injury and KOA only clarify the relationship between the two: subchondral bone injury is closely related to KOA, which can lead to the occurrence of KOA

But none of the models of subchondral bone injury have involved healing, let alone inducing local regeneration

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