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SSR (Simple Sequence Repeat) primesr of platygaster robiniae buhl and duso and application thereof to population genetic diversity analysis

A technology of genetic diversity and S. larvae, which is applied to the specific SSR primers of the locust leaf gall mosquito S. spp. and its application field, can solve the problems of origin, spread and genetic differentiation, etc., which are still blank.

Active Publication Date: 2018-09-28
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] At present, the research on the locust leaf gall midge is still in the stage of basic biological research, and its origin, spread and genetic differentiation are still blank

Method used

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  • SSR (Simple Sequence Repeat) primesr of platygaster robiniae buhl and duso and application thereof to population genetic diversity analysis
  • SSR (Simple Sequence Repeat) primesr of platygaster robiniae buhl and duso and application thereof to population genetic diversity analysis
  • SSR (Simple Sequence Repeat) primesr of platygaster robiniae buhl and duso and application thereof to population genetic diversity analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Design and screening of embodiment 1 specific SSR primer

[0057] 1 test material

[0058] Collect the damaged leaves of Robinia pseudoacacia in the wild and send them back to the laboratory, put them into jars for rearing, cover the mouth of the jar with three layers of gauze, and tie it tightly with rubber bands to prevent the bees from emerging and escaping; constant temperature (25°C) ) and constant humidity (70%), managed by full-time personnel, put the eclosion adults into centrifuge tubes containing absolute ethanol with a small brush every day, and store them at -20°C for later use.

[0059] 2 test method

[0060] 2.1 DNA extraction

[0061] The adults of Robinia pseudoacacia leaf gall midge, Leptospermia grandis, in Yingkou City, Liaoning Province were taken as the object, and the genomic DNA was extracted by referring to Wang Wen and Shi Liming (1993), and the collected genomic DNA was detected by 1% agarose gel electrophoresis.

[0062] 2.2 Genomic DNA Fra...

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Abstract

The invention discloses SSR (Simple Sequence Repeat) primers of platygaster robiniae buhl and duso and application thereof to population genetic diversity analysis. The invention designs according tomitochondrial genome data of the platygaster robiniae buhl and duso to obtain more than 130 thousand of SSR sequences and screens out 37 pairs of SSR specific primers which are relatively stable and have excellent polymorphism from the SSR sequences. The invention further discloses application of the 37 pairs of SSR specific primers in the aspects of population genetic diversity analysis, population evolution analysis and polymorphic analysis of the platygaster robiniae buhl and duso, construction of fingerprint spectrum of the platygaster robiniae buhl and duso and the like. The SSR primers and the application thereof have the important value for developing natural enemy class group population genetic diversity research of the platygaster robiniae buhl and duso, mastering the source and the population differentiation condition of the platygaster robiniae buhl and duso and the like.

Description

technical field [0001] The present invention relates to SSR primers, in particular to specific SSR primers for Platygaster robiniae Buhl and Duso, and the present invention further relates to the genetic diversity of the specific SSR primers in the population of Robinia robiniae Buhl and Duso It belongs to the field of specific SSR primers of Robinia locust leaf gall midge and its application in aspects such as sex analysis. Background technique [0002] Platygaster robiniae Buhl and Duso (Hymenoptera: Platygastridae) is an important alien species in China, Obolodiplosis robiniae (Haldeman) (Diptera: Gastrophyidae) egg-larva intertemporal parasitic natural enemies, The parasitism rate of Robinia pseudoacacia leaf gall midge is relatively high, especially in the later stage of its host year, the parasitism rate can often reach more than 80%, and it is a very good natural enemy resource. In the past ten years, the host of the locust leaf gall midge, Robinia pseudoacacia leaf ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2600/156C12Q2525/151
Inventor 姚艳霞杨君赵文霞淮稳霞林若竹
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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