A method for identifying the storage time of fresh mutton using dna and pcr techniques
A technology of storage time and technical identification, applied in the field of food safety testing, to achieve the effects of accurate results, simple operation and broad application prospects
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Embodiment 1
[0044] (1) Experimental materials
[0045] 1. Sample source
[0046] The mutton samples were goat legs that had been refrigerated for about 12 hours after slaughter and brought back in ice boxes. Store at -20°C (refrigerator temperature) for 11 months, 4°C (refrigerator temperature) for 5 days, 10°C (intermediate temperature) for 60 hours, and 20°C (room temperature) for 24 hours, ready to use.
[0047] 2. Test reagent
[0048] Mixed indicator (methyl red, methylene blue), DNA extraction buffer (NaCl 1mol / L, Tris-Cl 0.5mol / L, EDTA-Na 0.5mol / L, pH adjusted to 7.5-8.0), 5% SDS lysate (add 20g sodium dodecyl sulfate to 80mL double-distilled water, heat to dissolve at 68°C, adjust to pH=7.2 with concentrated HCl, and dilute to 100mL with double-distilled water), proteinase K (20mg / mL ), ice absolute ethanol (-20°C), ice ethanol aqueous solution (-20°C) with a volume fraction of 70%, TE buffer (2mL of 1mol / L Tris-HCl buffer with pH=8.0 and 0.4mL of 0.5mol / L EDTA aqueous soluti...
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