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Canine distemper genetic engineering subunit vaccine

A subunit vaccine, canine distemper technology, applied in genetic engineering, vaccines, veterinary vaccines, etc., can solve the problems of high cost and short duration of immune effect of inactivated vaccines, to prevent epidemics and spread, and achieve good protection effects , the effect of reducing economic losses

Inactive Publication Date: 2018-09-28
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new type of bacteria called Corynebactum (C.) catheterizes that causes encephalitis or paralysis from infected dogs with necrotic enteric lesions due to their high virus content. These microorganisms are able to cause harmful diseases such as feline leukemia which affecting over 20 million people worldwide every year causing significant financial losses for veterans who work alongside these animals. By administering an effective antigen-based vaccination program made up primarily of specific types of BCG cells it could help control this problem without requiring expensive medical treatment measures like surgery.

Problems solved by technology

There were several technical problem addressed during research into developing effective veterinary medicinal products against canin Distemper Virus Disease: Current methods involve giving up control over these types of illness through traditional measures like wearing masks containing protectants around their nostrils, reducing airborne transmission, avoidance of contact with horseshoe bats, and controlling environmental conditions like temperature and light intensity. However, current treatment options may lack effectiveness because they target only limited areas where cancers occur frequently.

Method used

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  • Canine distemper genetic engineering subunit vaccine
  • Canine distemper genetic engineering subunit vaccine
  • Canine distemper genetic engineering subunit vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Cloning of canine distemper virus H gene

[0017] The minks in a farm in Zhucheng City, Shandong Province are about 60 days old; they have not been immunized with canine distemper vaccine products for minks, and the minks have symptoms of suspected canine distemper, with obvious secretions from the eyes and nose, loss of appetite, and dry nose. However, there were no mink deaths, and it was suspected that they were infected by the attenuated strain of mink distemper virus. Virus screening was performed on the samples obtained from the farm, and finally a canine distemper virus CDV QN-1 strain was obtained, which was preserved in the Chinese Type Culture Collection Center located in Wuhan and Wuhan University on April 11, 2018. Deposit number: CCTCC NO:V201814;

[0018] Molecular biology identification According to the specific primers for amplifying the highly conserved sequence of the N gene, the expected amplified length is 265 bp; for a pair of specifi...

Embodiment 2

[0029] Embodiment 2: Construction of canine distemper virus H gene recombinant expression virus

[0030] The strains, bacterial strains, cells and plasmids required in this embodiment are as follows:

[0031] CDV QN-1 strain H gene fragment, Vero cells and pFastBac1 plasmid were all preserved by our laboratory; Escherichia coli E.coli DH5a was purchased from Treasure Bioengineering (Dalian) Co., Ltd.; Sf9 insect cells were obtained from China Animal Health and Epidemiological Control Center gift.

[0032] 1. Construction of recombinant expression vector pFastBac1-Head Domain

[0033] 1) Design 2 pairs of expression primers (F2, R2), and introduce BamHI and XhoI restriction sites and protective bases at the 5' end of the primers. The expected fragment size is 1921bp, PCR amplifies the Head Domain gene, and the target fragment and expression For double enzyme digestion of the vector pFastBac1, the gene fragment recovered from the gel was ligated with the pFastBac1 vector in an...

Embodiment 3

[0056] Example 3: Laboratory Trial Production of Canine Distemper Virus H Gene Subunit Vaccine and Product Quality Research

[0057] virus and cell

[0058] The virus seed used in the manufacture of this product is CDV recombinant baculovirus, constructed, kept and supplied by Qingdao Agricultural University, with a virus titer ≥ 10 7 IFU / ml; the virus species used for testing is CDV GN strain, ID 50 for 10 -2.0 / ml, isolated, identified, kept and supplied by Qingdao Agricultural University. The sixth generation of Sf9 production cells was identified, kept and supplied by Qingdao Agricultural University.

[0059] experimental animals

[0060] 6-month-old mink, the breeds are Golden State Black Standard Mink and American Shorthair Black Mink. The minks used in the test are in good health, with normal appetite and drink desire, no infectious diseases, and the neutralizing antibody titer is not higher than 1:4. Mink distemper has never occurred in the mink farm where it is ...

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Abstract

The invention provides a canine distemper genetic engineering subunit vaccine and a preparation method and application thereof. The canine distemper genetic engineering subunit vaccine comprises a vaccine adjuvant and an antigen. The antigen is an H protein having an amino acid sequence shown in the formula of SEQ ID NO: 2. The canine distemper subunit vaccine has stable biological characteristics, has good immunogenicity, is safe and reliable, has the very good protective effect on the leeches attacked by the canine distemper virulent GN strain, can effectively prevent the prevalence and spread of leech canine distemper after immunity, can reduce the economic losses caused by this disease and has broad application prospects.

Description

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Claims

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Application Information

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Owner QINGDAO AGRI UNIV
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