Method for culturing cord blood type embryonic stem cells as well as identification and application

Abstract

The invention discloses a method for culturing cord blood type embryonic stem cells as well as identification and application, relating to a method for culturing stem cells as well as identification and application. The culture method comprises: 1, collection of cord blood; 2, separation of the cord blood; 3, coating of a cord blood type embryonic stem cell culture dish; and 4, culture amplification of the cord blood type embryonic stem cells. The cord blood type embryonic stem cells are used for assisting recovery of injured lymphocytes. The formula of the culture medium adopts multiple cell factors to stably maintain the biological characteristic of cells and accelerate cell proliferation. The culture dish is coated by adopting laminin, and the laminin is beneficial to wall attachment of cells, so that the wall attachment time of the cells is effectively shortened, the adhesion degree of the cells to the bottom of the dish is improved and the cells are attached to the wall more firmly.

Description

Technical field [0001] The invention relates to a method for culturing stem cells, identification and application. Background technique [0002] Type I diabetes (formerly known as juvenile diabetes or insulin-dependent diabetes) is a type of diabetes. It has a completely different pathogenesis from type II diabetes. It is an autoimmune disease and may be caused by the destruction of the pancreatic islets β that produce insulin by the autoimmune system. Caused by cells. According to data released by the International Diabetes Federation (IDF) in 2009, there are currently approximately 30 million type 1 diabetes patients in the world. [0003] Type I diabetes mainly has the following prevalence factors: 1) Genetic factors: It is reported that about 25%-50% of people have the disease due to genetic factors. The genetic factors are relatively certain regardless of type I or type II. According to the study of modern twins, type I CCP dominance is 50%, and the rest are environmental fa...

AI Technical Summary

Problems solved by technology

Although the current research has confirmed that human embryonic stem cells can be induced to differentiate into islet-like cells in vitro, there are still many problems: 1) The host has immune rejection to hES differentiated cells; 2) The induction differentiation efficiency is low and the instability is low; 3 ) Maturity of differentiated cells with potential tumorigenicity

4) The source of embryos involves ethical and moral issues, so research and application are limited, so it has little clinical significance in the near future

Problems in the clinical application of adult stem cells: 1) The access to adult stem cells is limited, the number is small and the proliferation ability is limited; 2) The lack of specific marker molecules makes it difficult to separate, identify, and purify; 3) The differentiation ability is limited and has large variations Degree; lack of effective directed differentiation program, poor maturity of differentiated cells

However, there is still a lack of major technological breakthroughs in these areas

[0008] Although the current clinical studies on stem cell therapy for type 1 diabetes have shown certain effects in controlling blood sugar levels, they all have the problem of being unable to correct the abnormal immune function of patients. Therefore, these methods only have short-term blood sugar regulation effects and cannot guarantee long-term effects.

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