Methods to construct and use animal model to study anti-inflammatory effect of spirulina extract

A technology for spirulina extraction and anti-inflammatory effect, which can be used in anti-inflammatory agents, preparations for in vivo experiments, drug combinations, etc., and can solve problems such as glucocorticoid mental disorders and allergic rashes

Inactive Publication Date: 2018-09-21
GUANGXI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Glucocorticoid is a hormone drug secreted by the adrenal gland that has a powerful and rapid anti-inflammatory e

Method used

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  • Methods to construct and use animal model to study anti-inflammatory effect of spirulina extract
  • Methods to construct and use animal model to study anti-inflammatory effect of spirulina extract
  • Methods to construct and use animal model to study anti-inflammatory effect of spirulina extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 animal feeding and processing method

[0051] After feeding 70 clean-grade Kunming mice with an average weight of 18-22g for 3 days, they were randomly divided into 7 groups, with 10 mice in each group, half male and half female, and a blank control group, a positive control group, and spirulina extract were added. Group (0.05%, 0.1%, 0.2%, 0.3%, 0.4%). The specific grouping and treatment plan are shown in Table 1.

[0052] Table 1 Grouping and treatment plan

[0053]

Embodiment 2

[0054] The establishment of embodiment 2 inflammation model

[0055] The mice were weighed once before administration and inflammation. One hour after the last administration, smear 0.05 ml of xylene evenly on both sides of the base of the left ear of the mouse with a 1 mL syringe, and wait for 20 min. Then, a 6mm hole punch was used to punch ear pieces at the same position on the left and right ears of the mice, and the mice were weighed quickly. Calculate the ear swelling degree, ear swelling rate and swelling inhibition rate of the mice. Each calculation method is as follows:

[0056] Mouse ear swelling = left ear mass - right ear mass;

[0057] Ear swelling rate = (left ear mass - right ear mass) / right ear mass;

[0058] Swelling inhibition rate=(average swelling degree of blank group-average swelling degree of medication group) / average swelling degree of blank group×100%.

[0059] The effect results of Spirulina extract on ear swelling test mouse ear swelling are s...

Embodiment 3

[0065] Example 3 Observation of the Effect of Spirulina Extract on the Organ Index of Mice

[0066] After causing inflammation and removing the mouse ears, the mice were dissected, and the tissues and organs were observed with the naked eye. The liver, kidney and thymus of the mice were removed for weighing, and the indexes of each organ were recorded and calculated. The calculation method is as follows:

[0067] Organ index = mass of each organ / mouse body weight.

[0068] Table 3 The effect of spirulina extract on the organ index of mice

[0069]

[0070] Note: "**" means that compared with the blank control group, the difference is extremely significant (P<0.01). The same below.

[0071] as table 3 and Figure 2-Figure 4 As shown, the liver index of the positive control group was significantly higher than that of the blank control group (P<0.01).

[0072] The results showed that there was no significant difference in liver index, kidney index, spleen index, and thymu...

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Abstract

The invention discloses a method to construct an animal model to study anti-inflammatory effect of spirulina extract, namely to construct a verification model of mouse acute ear swelling by using xylene as inflammatory agents. The contents of IL-2, IL-6, IL-1 beta, TNF-alpha and IFN-gamma in serum of a model mouse are detected; mouse organ indexes are recorded; pathological tissue slices of thymusand spleen are made; tissue structural changes of the thymus and spleen are observed in terms of histomorphology; anti-inflammatory effect of spirulina extract is evaluated. The study shows that theanti-inflammatory effect can partly increase the IL-2 and IL-6 levels in mice and reduce the IL-1 beta level in mice, plays a certain positive role of restoring TNF-alpha and IFN-gamma in mice to normal levels, and therefore, inhibits related pathological injury processes and relieves inflammatory injury. Therefore, the method can be used to provide basis for the deep development and utilization and clinical application of spirulina.

Description

technical field [0001] The invention belongs to the technical field of spirulina development, and in particular relates to a method for constructing and using an animal model for studying the anti-inflammatory effect of spirulina extract. Background technique [0002] Inflammation is a defensive response of the animal body to adverse stimuli. Sometimes, the inflammatory response is beneficial to the body, but more often it is harmful. Inflammatory response is the basis for the occurrence and development of many diseases. With the progress of inflammatory response, many diseases begin to appear and cause damage to the body. Therefore, the anti-inflammatory effect is one of the key directions of many drug development and research. Glucocorticoid is a hormone drug secreted by the adrenal gland that has a powerful and rapid anti-inflammatory effect. However, glucocorticoid has adverse reactions such as mental disorders, immune suppression, and allergic rashes. In recent years,...

Claims

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Application Information

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IPC IPC(8): A61K31/015A61K35/748A61P29/00A61K49/00
CPCA61K31/015A61K35/748A61K49/0008A61P29/00
Inventor 胡庭俊谷俐媛陈忠伟梁万文何颖韦英益施君邱洁
Owner GUANGXI UNIV
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