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A kind of analysis method of nicotine and cotinine in urine

An analytical method and nicotine technology, applied in analytical materials, material separation, instruments, etc., can solve the problems of low degree of automation, cumbersome operation, batch analysis, etc., and achieve the effect of convenient analytical method, good repeatability and high sensitivity

Active Publication Date: 2021-08-17
SHANGHAI TOBACCO GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, urine pretreatment is generally carried out with commercially available solid phase enrichment (SPE) cartridges, but the steps of loading and elution are cumbersome, the degree of automation is not high, and the process takes a long time, which is not conducive to batch analysis of large-scale samples

Method used

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  • A kind of analysis method of nicotine and cotinine in urine
  • A kind of analysis method of nicotine and cotinine in urine
  • A kind of analysis method of nicotine and cotinine in urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] 1. Experimental device

[0094]The present invention provides an on-line analysis system for nicotine and cotinine in urine, comprising an enrichment device, a gas chromatograph, and a mass spectrometer connected sequentially through pipelines, and the enrichment device includes a thermostatic cup, an enrichment packed column , a switch valve, the enrichment packed column and the switch valve are placed in a constant temperature cup, the inlet and outlet of the enriched packed column are respectively connected with the switch valve through pipelines, and the switch valve is also connected through pipelines There are auxiliary gas and carrier gas externally connected respectively. Wherein, the switching valve is a six-way valve, and the six-way valve is provided with a first port, a second port, a third port, a fourth port, a fifth port, and a sixth port sequentially in a counterclockwise direction. A control valve is provided on the pipeline between the discharge port ...

Embodiment 2

[0112] 1. Experimental device

[0113] An online analysis system for nicotine and cotinine in urine used in this embodiment is the same as the experimental device in 1 of embodiment 1.

[0114] Wherein, the enrichment packed column is a stainless steel tube with an outer diameter of 6 mm and an inner diameter of 4 mm. The enriched packed column is filled with mesoporous material, the two ends of the enriched packed column are filled with glass fibers, and the chemical composition of the mesoporous material is selected from pure SiO 2 Or one of pure carbon or a mixture of both. The mesoporous material is a mesoporous ball with a pore diameter of 2-5nm. The mesoporous material needs to be pretreated. The pretreatment condition of the mesoporous material is roasting in 100% water vapor at 700°C for 6h. The glass fiber is deactivated glass fiber, and the filling height of the glass fiber at one end of the enrichment packed column is 5mm. The filling amount of the mesoporous mat...

Embodiment 3

[0131] Get the standard solution containing the nicotine of internal standard substance and cotinine, prepare the standard sample containing internal standard substance by 3 in embodiment 2, and measure according to the chromatographic condition of 2 in embodiment 2, contain internal standard substance In the standard solution of nicotine and cotinine, the concentration of nicotine is 2000ng / mL, the concentration of cotinine is 2000ng / mL, and the concentration of internal standard n-hexadecane is 100ng / mL.

[0132] During the measurement, the filling amount of the mesoporous material in the enrichment packed column was 0.1 g. See Table 1 for the adsorption and desorption of typical exposure markers nicotine and cotinine in the urine of smokers. Electron diagram of mesoporous materials such as Figure 3a , 3b shown. From Table 1, Figure 3a , 3b It can be seen that the mesoporous material selected in the present invention has excellent adsorption and desorption properties....

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Abstract

The invention provides a method for analyzing nicotine and cotinine in urine, which includes the following steps: 1) Take a standard solution of nicotine and cotinine containing an internal standard, inject it into an enrichment device and carry out the process under auxiliary gas purging Adsorption enrichment, after thermal desorption under carrier gas purging, a standard sample containing an internal standard is obtained; 2) Take a urine sample, and use the same steps as step 1) to obtain a sample to be tested; 3) Separately The standard sample and the test sample containing the internal standard substance enter the GC-MS under the purging of the carrier gas for detection, compare the retention time according to the obtained mass spectrum for qualitative determination, determine the nicotine and cotinine components in the test sample, and The content of nicotine and cotinine in the sample to be tested is calculated by internal standard method. The method for analyzing nicotine and cotinine in urine provided by the invention is convenient, efficient, fast, high in sensitivity and good in repeatability, and can fully meet the analysis requirements of nicotine and cotinine in urine.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical detection of tobacco components, and relates to an analysis method for nicotine and cotinine in urine. Background technique [0002] As people's calls for health are getting higher and higher, the issue of "smoking and health" has become the focus of attention of the public and the World Health Organization. risk, it is of great significance to produce low-harm or low-risk cigarette products. On the other hand, although the global tobacco control campaign is in full swing, the current smoking population is still huge, and it is difficult to completely disappear in the short term. At this time, the only way is to objectively evaluate the risks of cigarettes, and on this basis, vigorously develop harm reduction and risk assessment technologies to reduce the health risks of smokers. [0003] Nicotine and cotinine are the most commonly used and most important smoke exposure markers. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/08G01N30/06
CPCG01N30/02G01N30/06G01N30/08G01N2030/045
Inventor 李钢汪阳忠蔡振波陶立奇
Owner SHANGHAI TOBACCO GRP CO LTD
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