Cell fixation liquid for flow detection and use method thereof
A cell fixation and flow detection technology, applied in the preparation of test samples, measurement devices, sampling, etc., can solve the problems of short storage time and many cell debris.
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specific Embodiment approach 1
[0015] Specific embodiment one: In this embodiment, a cell fixative solution for flow detection is mixed by paraformaldehyde, glutaraldehyde, phosphate buffer and deionized water; wherein the mass concentration of paraformaldehyde in the fixative solution is 0.3-0.5%, the mass concentration of glutaraldehyde is 0.3-0.5%, and the final concentration of phosphate buffer is 0.01-0.03M.
[0016] In this embodiment, the process of glutaraldehyde immobilizing proteins through cross-linking will be accompanied by the release of hydrogen ions, which will lower the pH value of the sample. In order to keep the pH stable at an optimal level, a sufficient amount of buffer should be added to the fixative of glutaraldehyde, therefore, corresponding phosphate buffer should be added to the fixative. Glutaraldehyde can fix DNA and RNA in tissues and cells by fixing nucleoproteins, can preserve glycogen, and can also fix lipids associated with proteins or containing amino and imino groups, and ...
specific Embodiment approach 2
[0017] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the mass concentration of paraformaldehyde in the stationary solution is 0.4%. Others are the same as those in Embodiment 1 or 2.
specific Embodiment approach 3
[0018] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the mass concentration of glutaraldehyde in the stationary solution is 0.4%. It is the same as the specific embodiment 1 or 2.
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