Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving chemiluminescence immune assay precision of magnetic particles of core antibodies

A chemiluminescent immunoassay and core antibody technology, applied in the field of immunoassay, can solve the problems of repeated detection fluctuations, detection precision interference, etc., and achieve the effect of improving precision, improving precision, and reducing quantitative differences

Active Publication Date: 2018-09-11
捷和泰(北京)生物科技有限公司
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] After protein C self-assembles into virus-like particles in vitro, there are still a considerable number of free protein C dimers, and once the virus-like particles assembled by non-covalent interactions are damaged by external forces, self-assembly and disassembly will occur. The balance of assembly is tilted towards the direction of disassembly, and the actual epitopes on the virus-like particles are less than the theoretical epitopes in the end, and there will be fluctuations between repeated detections, which seriously interferes with the detection precision

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving chemiluminescence immune assay precision of magnetic particles of core antibodies
  • Method for improving chemiluminescence immune assay precision of magnetic particles of core antibodies
  • Method for improving chemiluminescence immune assay precision of magnetic particles of core antibodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A core antibody magnetic particle chemiluminescence immunoassay kit, comprising: M reagent, R1 reagent and R2 reagent;

[0038] (1) M reagent

[0039] Including polyglycine-coated magnetic particles, core antigen-coated magnetic particles and magnetic particle buffer; the magnetic particle buffer is 0.02MpH7.5 HEPES buffer, polyglycine-coated magnetic particles and core antigen-coated The magnetic particles are mixed in the magnetic particle buffer, the final concentration of polyglycine-coated magnetic particles is 0.4 mg / ml, and the final concentration of core antigen-coated magnetic particles is 0.2 mg / ml.

[0040] 1) The reaction system of polyglycine-coated magnetic particles is: coating buffer, magnetic particles, glycine, EDC and magnetic particle buffer;

[0041] Coating buffer: MES buffer at 0.05MpH6.0;

[0042] Magnetic particles: MS300carboxyl magnetic particle suspension (100mg / ml) provided by JSR Corporation;

[0043] Glycine: Glycine provided by BBI Cor...

Embodiment 2

[0065] The influence of M reagent containing polyglycine-coated magnetic particles and M reagent without polyglycine-coated magnetic particles on the precision of core antibody immunoassay was compared.

[0066] Experimental group: the core antibody magnetic particle chemiluminescence immunoassay kit in Example 1.

[0067] Control group: M reagent includes core antigen-coated magnetic particles and magnetic particle buffer; the composition, ratio and preparation method of the core antigen-coated magnetic particles and magnetic particle buffer are the same as in Example 1. R1 and R2 reagents are the same as in Example 1.

[0068] Three human serum samples with known HBcAb(+) and three human serum samples with known HBcAb(-) were tested with reagents in the experimental group and the control group, among which M reagent 50uL, R1 reagent 50uL, R2 reagent 100uL, sample 50uL , detected by a fully automatic chemiluminescence immunoassay analyzer, each sample was tested 10 times, an...

Embodiment 3

[0075] The effects of M reagent containing polyglycine-coated magnetic particles and M reagent containing fish gelatin-coated magnetic particles on the precision of core antibody immunoassay were compared.

[0076] Experimental group: the core antibody magnetic particle chemiluminescence immunoassay kit in Example 1.

[0077] Control group:

[0078] M reagent includes fish gelatin-coated magnetic particles, core antigen-coated magnetic particles and magnetic particle buffer.

[0079] The preparation process of fish gelatin-coated magnetic particles is as follows: add magnetic particles, fish gelatin and EDC sequentially to the coating buffer solution without magnetic stirring, the ratio of fish gelatin to magnetic particles is 10ug / mg, and the ratio of EDC to magnetic particles is 1mg / mg mg. React overnight at 2-8°C, collect magnetism for 2 minutes, discard the liquid, add an equal volume of magnetic particle buffer to wash, repeat this 3 times, finally collect magnetism, di...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving the chemiluminescence immune assay precision of magnetic particles of core antibodies. The method disclosed by the invention has the beneficial effects that by mixed use of magnetic particles coated by polyglycine and magnetic particles coated by core antigens, relative balance of the self-assembly rate and the disassembly rate of virus-like particlesis maintained, the influences of various mechanical forces in the detection process on the electrostatic action, the hydrophobic action and hydrogen bonds depended by self assembly of the virus-likeparticles is reduced, further the actual number of antigen epitopes on the virus-like particles is ensured to be close to the theoretical number, the difference of the quantity of antibody markers ofthe virus-like particles bonded on solid-phase carriers in multiple repeated detection is reduced, and the precision of immunoassay of the core antibodies is improved. Furthermore, based on the method, the invention also provides a kit for chemiluminescence immune assay of the magnetic particles of the core antibodies, so that the immune assay precision of the core antibodies can be obviously improved.

Description

technical field [0001] The invention relates to the technical field of immunoassay, and more specifically relates to a method for improving the precision of core antibody magnetic particle chemiluminescence immunoassay. Background technique [0002] Hepatitis B is one of the most widespread contagious public health problems in the world. The hepatitis B virus has infected 2 billion people in the world, of which 360 million people have become chronic hepatitis B, causing about 600,000 deaths each year. . In the process of infecting the human body, the hepatitis B core antigen (HBcAg) is the most immunogenic part of the hepatitis B virus. It is a particle composed of multiple copies of the C protein and is the nucleocapsid of the hepatitis B virus. Under the electron microscope, the nucleocapsid exhibits an icosahedral topology. All patients who have been infected with hepatitis B virus will produce high titer core antibody (anti-HBc), and regardless of the prognosis, the an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/576G01N21/76
CPCG01N21/76G01N33/56983G01N33/5761G01N33/5762G01N2333/005
Inventor 翁宇
Owner 捷和泰(北京)生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com