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Serratia marcescens strain and application thereof to production of ligninolytic enzyme and degradation of lignin

A technology of Serratia marcescens and lignin degrading enzyme, applied in the field of lignin degradation

Inactive Publication Date: 2018-09-07
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the enzymes that insect intestinal bacteria can produce are 1,4-β-endoglucanase, 1,4-β-xylanase, pectinase, α-amylase, etc. Promote the digestion and absorption of nutrients such as lignin, xylan, pectin and starch in the insect gut, but there are very few studies on the decomposition of lignin by lignin-degrading enzymes in bacteria isolated from the insect gut

Method used

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  • Serratia marcescens strain and application thereof to production of ligninolytic enzyme and degradation of lignin
  • Serratia marcescens strain and application thereof to production of ligninolytic enzyme and degradation of lignin
  • Serratia marcescens strain and application thereof to production of ligninolytic enzyme and degradation of lignin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Strain isolation and preservation

[0044] The tested strain of Serratia marcescens was isolated from the intestinal tract of Monochamus alternata larvae. The larvae of Monochamus alternata come from the masson pine forest (N26.150°; E119.593°) around Zhongbang Reservoir, Guantou Town, Lianjiang County, Fujian Province, and are collected by splitting the masson pine wood. The isolated Serratia marcescens strain is preserved in the China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCCNO.15175.

[0045] (2) Expansion of bacterial strains

[0046] A single colony of the preserved Serratia marcescens was inoculated into a sterilized SOC medium, and placed in a 30° C. constant temperature incubator under dark conditions for activation culture to obtain an expanded culture of Serratia marcescens strains. SOC medium comprises the following content components: 2% (W / V) tryptone, 0.5% (W / V) yeast extract, 0.05% (W / V) NaC...

Embodiment 2

[0070] The Serratia marcescens bacterial strain SM01 preserved in Example 1 was obtained after expanded culture, and the bacterial liquid was inoculated into the liquid enzyme-producing medium with an inoculum size of 5%, and the liquid enzyme-producing medium was Kraft lignin 10.0g / L; (NH 4 ) 2 SO 4 2.0g / L; K 2 HPO 4 0.5g / L; KH 2 PO4 1.5g / L; MgSO 4 0.6g / L; CaCl 2 0.2g / L; FeSO 4 0.05g / L; MnSO 4 0.02g / L; Yeast extract is 2g / L; The concentration of described urea is preferably 5g / L. The pH of the medium is 6.5. Continuous culture was carried out at 30° C. for 4 days at a rotational speed of 200 rpm, and the lignin degradation rate was measured according to the method in Example 1. Centrifuge the cultured bacterial solution at 4°C for 5 minutes at a speed of 10,000 g, and take the supernatant, which is the crude enzyme solution. The obtained crude enzyme liquid was assayed for enzyme activity according to the method in Example 1. The results are shown in Table 2.

Embodiment 3

[0072] The Serratia marcescens strain SM01 isolated in Example 1 is obtained after expanded culture, and the bacterial liquid is inoculated into the liquid enzyme-producing medium with an inoculum size of 8%, and the liquid enzyme-producing medium is Kraft lignin 1.0g / L; (NH 4 ) 2 SO 4 2.0g / L; CaCl 2 0.5g / L; FeSO 4 0.2g / L; MnSO 4 0.06g / L; Yeast extract is 6g / L; The concentration of described urea is preferably 3g / L. The pH of the medium is 5.0. Continuous culture was carried out at 30° C. for 4 days at a rotational speed of 200 rpm, and the lignin degradation rate was measured according to the method in Example 1. Centrifuge the cultured bacterial solution at 4°C for 5 minutes at a speed of 10,000 g, and take the supernatant, which is the crude enzyme solution. The obtained crude enzyme liquid was assayed for enzyme activity according to the method in Example 1. The results are shown in Table 2.

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Abstract

The invention provides a serratia marcescens strain and application thereof to production of a ligninolytic enzyme and degradation of lignin and belongs to the technical field of lignin degradation. The serratia marcescens strain SM01 (Serratia marcescens) has a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO. 15175. The serratia marcescens strain providedby the invention is applied to the production of the ligninolytic enzyme. The serratia marcescens strain provided by the invention is applied to the degradation of the lignin. The serratia marcescensstrain has an efficient lignin degradation capability and the accumulated degradation rate of the lignin reaches 92 percent or more.

Description

technical field [0001] The invention belongs to the technical field of lignin degradation, and in particular relates to a Serratia marcescens strain and its application in producing lignin degrading enzyme and degrading lignin. Background technique [0002] Lignin is the main source of nutrition for the larval stage of tree borer pests, and plays an important role in the growth and development of this kind of insects. Among them, lignin is the main component of lignin, which is a macromolecular substance with stable properties, insoluble in water and most solvents, and cannot be directly absorbed and operated by insect intestinal wall cells. Many studies have shown that insect gut microbes play an important role in the biodegradation of lignin. They promote lignin cleavage reactions by producing a variety of extracellular enzymes to form small molecules that can be absorbed. [0003] At present, most of the studies on lignin-degrading enzymes produced by insect gut microbes...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/02C12N9/08C12P1/00C12R1/425
CPCC12N9/0061C12N9/0065C12P1/00C12Y110/03002C12Y111/01013C12Y111/01014C12N1/205C12R2001/425
Inventor 胡霞傅慧静张飞萍李明吴松青梁光红王荣罗巧玉陈昊
Owner FUJIAN AGRI & FORESTRY UNIV
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