Paenibacillus macerans and straw decomposing agent

A straw decomposing agent and Bacillus technology, applied in the directions of bacteria, biochemical equipment and methods, applications, etc., can solve the problems of high environmental requirements, unstable decomposition effect, and reduced decay-inducing effect, and achieve the goal of killing bacteria, The effect of shortening the decomposing time and stabilizing the decomposition effect

Active Publication Date: 2018-09-07
四川明湖环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the existing straw decomposing agents on the market require a long time to decompose. The straw needs to be piled up, the operation is complicated, the decomposition effect is unstable, and the requirements for the environment are very high. In case of low temperature or rain, the rot will be accelerated. The effect is obviously reduced, and there is a singleness, so it cannot be widely used
For example, the components of the decomposing agent disclosed in the invention patent titled "a straw decomposing agent" (application number 201310666396.9) include the following active components: Leather, as well as Geobacillus stearothermophilus, have a large number of components, require a preparation process, and the preparation process is complicated, which brings difficulties to the promotion and application of enterprises.
Therefore, the research on the decomposing agent with good effect and relatively simple preparation method needs to be deepened.

Method used

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  • Paenibacillus macerans and straw decomposing agent
  • Paenibacillus macerans and straw decomposing agent
  • Paenibacillus macerans and straw decomposing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the preparation of Paenibacillus softening fermented liquid

[0033] (1) Strain activation: Inoculate Paenibacillus softening into slant medium (peptone 10g, yeast extract 2g, beef extract 5g, sodium chloride 5g, agar 20g, replenish water to 1000ml, pH7.2), keep the temperature at 35°C Cultivate in the incubator for 24h;

[0034] (2) Shake flask culture: inoculate the Paenibacillus softening bacillus activated in step (1) in the liquid medium (peptone 10g, yeast extract 2g, beef extract 5g, sodium chloride 5g, replenish water to 1000ml, pH7.2) , cultured on a shaker at 35°C and 130rmp for 24h;

[0035] (3) seed tank fermentation: inoculate the Paenibacillus softening culture solution in the step (2) by 5% inoculum in the seed tank, and cultivate the effective viable count of bacterial classification in the Paenibacillus softening culture solution to be 2 * 10 8 Individual / ml, the liquid culture medium formula in the seed tank culture medium and its step ...

Embodiment 2

[0037] Embodiment 2: the preparation of Bacillus subtilis fermented liquid

[0038] Source of strain: Bacillus subtilis is a commercially available strain, purchased from China Industrial Microorganism Culture Collection Management Center, preservation number CICC No.10088; Address: Building 6, Yard 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing , zip code: 100015;

[0039] (1) Strain activation: Inoculate Bacillus subtilis in slant medium (peptone 10g, yeast extract 2g, beef extract 5g, sodium chloride 5g, agar 20g, replenish water to 1000ml, pH7.0), and culture at 35°C Cultivate in the box for 24h;

[0040] (2) shake flask culture: inoculate the Bacillus subtilis activated in the step (1) in the liquid medium (peptone 10g, yeast extract 2g, beef extract 5g, sodium chloride 5g, replenish water to 1000ml, pH7.2), Under the conditions of 35°C and 130rmp, shaking culture was carried out for 24h;

[0041] (3) Seed tank fermentation: the Bacillus subtilis nutrient solu...

Embodiment 3

[0043] Embodiment 3: the preparation of straw decomposing agent

[0044] Mix the Paenibacillus softening bacteria liquid prepared in Example 1 and the Bacillus subtilis bacterial liquid prepared in Example 2 at a volume ratio of 2:1 to obtain a mixed bacterial liquid, and mix the mixed bacterial liquid with the crushed straw with a size no more than 2cm in 3 : 7 wet weight ratio mixing and stirring, naturally air-dried, crushed through a 20-mesh sieve, and prepared to obtain a straw decomposing agent, the total effective number of viable bacteria of this straw decomposing agent is about 10 8 pcs / g.

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Abstract

The invention discloses a straw decomposing agent which comprises components of Paenibacillus macerans CGMCC No. 14564 and Bacillus subtilis CICC No. 10089; the total effective viable cell count in the decomposing agent is 0.5*10<8>-2*10<8> / g, and the effective viable cell count of the Paenibacillus macerans is 65%-70% of the total effective viable cell count; the invention also discloses a preparation method of the straw decomposing agent, compared with use of various strains to prepare the decomposing agent in the prior art, the preparation method has a small variety of raw materials, and has a simple preparation process, the Bacillus subtilis whoch is not used in the prior art is used in the preparation method as one of the raw materials to interact with the Paenibacillus macerans, andthe use effect is remarkable, so that the preparation method can be promoted in related enterprises.

Description

technical field [0001] The invention relates to the technical field of decomposing agent preparation, in particular to a straw decomposing agent and a preparation method thereof. Background technique [0002] Crop straw is a biological resource produced in the process of crop production. Since the 1980s, with the substantial increase in grain production, the amount of straw has also increased rapidly, and the output of grain crop straw has increased from 4.6×108t in 2001 to 5.8×108t in 2010. With the promotion and popularization of clean energy, there is a large amount of surplus straw in agriculture. Every year, most corn, wheat and other crop stalks are burned, and a large amount of CO and CO are produced during the incineration process. 2 , Straw burning will not only pollute the air, but also destroy the soil structure and soil microbial flora. [0003] As an advanced conservation tillage technology, straw returning to the field has received enough attention from the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/00C12R1/01C12R1/125
CPCC12N1/20C05F17/20C12N1/205C12R2001/01Y02W30/40
Inventor 周玉珍张荣贵李鹏辉
Owner 四川明湖环保科技有限公司
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