Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of a kind of artificial cornea

A cornea, artificial technology, applied in the field of biomedicine, to achieve the effect of strong applicability, novel preparation method and wide application potential

Active Publication Date: 2020-11-06
JINAN PANSHENG BIOTECH
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of precise and effective artificial corneas that will not be rejected by the human immune system is a great challenge for related research fields.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of a kind of artificial cornea
  • Preparation method and application of a kind of artificial cornea
  • Preparation method and application of a kind of artificial cornea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Preparation of artificial cornea by sheep autologous keratocytes

[0026] 1. Collection and in vitro expansion and culture of corneal epithelial cells

[0027] (1) The cornea of ​​a sheep suffering from corneal disease was surgically excised, the epithelial cells were separated, and washed twice with DMEM containing 1× double-antibody;

[0028] (2) Take a 60mm cell culture dish, add the prepared Matrix (hESC-qualified Matrix human embryonic stem cell serum-free culture medium) into the culture dish and spread evenly, let it stand at room temperature for 10min, then discard the excess liquid in the culture dish Lose.

[0029] (3) Resuspend the corneal epithelial cell pellet with 4ml DF-K medium, inoculate it in the 60mm cell culture dish coated in step (2), name it AES170504P0, put the cell culture dish in 37℃5%CO 2 cultured in a cell culture incubator;

[0030] (4) Cell observation and photo recording: observe the growth status of the cells under a microsc...

Embodiment 2

[0106] Example 2. Preparation of artificial cornea by human autologous keratocytes

[0107] The discarded corneas of the recipients during the treatment of corneal diseases were collected, and the human cornea-related cells were expanded and cultured in vitro. After the discarded human cornea was obtained from the hospital, the corneal epithelial cell layer, Descemet's layer and stroma were separated from the Descemet's layer and corneal endothelial cells in a sterile ultra-clean workbench in the laboratory. Then, the human corneal epithelial cells and corneal endothelial cells were separated by mixed enzyme method. The separation process can adopt the prior art. Afterwards, human corneal epithelial cells and human corneal endothelial cells were expanded and cultured in vitro. When the human corneal epithelial cells and human corneal endothelial cells are cultured to the third generation, the preparation of human artificial cornea can be started. This part of the method is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention provides a preparation method and an application of an artificial cornea. According to the method, a cornea is separated in vitro, and corneal epithelial cells and corneal endothelial cells are separated out and then subjected to in-vitro amplification culture; preparation of the artificial cornea is started when the corneal epithelial cells and corneal endothelial cells pass to thethird generation; an epithelial cell layer, a Bowman's layer and a stroma layer of the artificial cornea are prepared from the third-generation corneal epithelial cells; a Descemet's membrane and anendothelial cell layer are prepared from the third-generation corneal endothelial cells as the endothelial cells; then the artificial cornea is prepared from the epithelial cell layer, the Bowman's layer, the stroma layer, the Descemet's membrane and the endothelial cell layer. The artificial cornea is prepared with the method according to the cornea structure forming process, functions of the artificial cornea are consistent with those of a receptor corner, immunological rejection can be avoided, and the applicability is high.

Description

technical field [0001] The invention provides a preparation method and application of a novel artificial cornea, belonging to the field of biomedicine. Background technique [0002] The cornea is the transparent part at the front of the eye. It is the focusing lens of the eye. It covers the iris, pupil and anterior chamber of the eye. It provides most of the refractive power for the eye and gathers the light. The light can form an image on the retina at the back of the eye. . And there are very sensitive nerve endings on the cornea. If a foreign object touches the cornea, the eyelids will involuntarily close to protect the eyes. There are no blood vessels on the cornea, so it appears transparent, and it obtains nutrients and oxygen through tears and aqueous humor. [0003] If the cornea is damaged or swollen due to corneal inflammation, physical injury, mechanical injury, chemical injury or congenital corneal abnormalities, it will cause severe vision loss, and even blindn...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/24A61L27/50C12N5/079
CPCA61L27/24A61L27/3808A61L27/3813A61L27/3891A61L27/3895A61L27/50A61L2430/16C12N5/0621C12N2533/30
Inventor 吴训伟邢志青王杰张平张甜甜李霄王红丽
Owner JINAN PANSHENG BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com