Northern corn leaf blight efficient disease resistance identification inoculant and preparation method thereof

A technology for maize large spot and inoculum, which is applied in the field of plant pathogenic fungi research, can solve the problems of inability to determine single-cell pure strains, unsatisfactory disease resistance identification results, and inconvenient disease resistance identification operations, so as to accelerate growth and improve yield. The amount of spores and the effect of easy mixing and uniformity

Inactive Publication Date: 2018-08-28
GUANGXI AGRI VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method still utilizes plate culture medium to carry out sporulation, and its sporulation, take sporulation operation process is loaded down with trivial details, is unfavorable for the acquisition of a large amount of conidia, is not convenient to carry out large-area disease-resistant identification operation, can't inoculate with pathogen-carrying medium
[0004] In the disease resistance identification test, usually randomly isolated Pythora maize is used as the test strain, but sometimes the strains used are not strong in pathogenicity, and the effect of disease resistance identification is not ideal; in the process of pathogen isolation, dilute Single-spore isolation by method, it is not sure whether a single-cell pure strain is obtained
In the preservation of pathogens, PDA medium is often used for cultivation and preservation, but because of the high nutrition of PDA medium, the growth of pathogens is vigorous, and the vitality of the strains is easy to decline after preservation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation method of the high-efficiency disease-resistant identification inoculum of maize leaf spot, the steps are as follows:

[0033] 1) For the screening of strong pathogenic strains of corn leaf spot, first isolate the pathogens, then purify them with single cells, use Koch's law to verify the pathogens, and finally screen out the strong pathogenic strains through the determination of the pathogenicity of conventional strains. Virulent strains, high-purity, strong pathogenicity, and high vigor strain HD12 were obtained.

[0034]Among them, the single-cell purification method adopts the labeling method for single-cell purification. The specific steps are: pour 10ml of thin-layer PDA medium into a petri dish, spread the diluted spore suspension on the medium, and the spore suspension is at 100 The concentration of 1-2 spores per field of view under a magnification lens, seal the cover of the culture dish with a sealing film, and move it into an incubator at 26-...

Embodiment 2

[0041] The preparation method of the high-efficiency disease-resistant identification inoculum of maize leaf spot, the steps are as follows:

[0042] 1) For the screening of strong pathogenic strains of corn leaf spot, first isolate the pathogens, then purify them with single cells, use Koch's law to verify the pathogens, and finally screen out the strong pathogenic strains through the determination of the pathogenicity of conventional strains. Virulent strains, high-purity, strong pathogenicity, and high vigor strain HD12 were obtained.

[0043] Wherein, the single cell purification method adopts the labeling method for single cell purification, and the specific steps are the same as in Example 1.

[0044] 2) Put the screened highly pathogenic strains into PCA slant medium for 4-7 days and store them in a 4°C refrigerator. The formula of the PCA medium is: 20g of potatoes, 20g of carrots, 16-20g of agar, and 1000ml of water.

[0045] 3) Preparation of secondary strains: Put...

Embodiment 3

[0050] The preparation method of the high-efficiency disease-resistant identification inoculum of maize leaf spot, the steps are as follows:

[0051] 1) For the screening of strong pathogenic strains of corn leaf spot, first isolate the pathogens, then purify them with single cells, use Koch's law to verify the pathogens, and finally screen out the strong pathogenic strains through the determination of the pathogenicity of conventional strains. Virulent strains, high-purity, strong pathogenicity, and high vigor strain HD12 were obtained.

[0052] Wherein, the single cell purification method adopts the labeling method for single cell purification, and the specific steps are the same as in Example 1.

[0053] 2) Put the screened highly pathogenic strains into PCA slant medium for 4-7 days and store them in a 4°C refrigerator. The formula of the PCA medium is: 20g of potatoes, 20g of carrots, 16-20g of agar, and 1000ml of water.

[0054] 3) Preparation of secondary strains: Put...

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Abstract

The invention provides a northern corn leaf blight efficient disease resistance identification inoculant and a preparation method thereof. The inoculant is prepared from the following raw materials: 100g of wheat, 2.0-3.0g of mannitol and 10-25g of corn leaves. The preparation method comprises the following steps: 1) screening northern corn leaf blight highly-pathogenic strains; 2) preserving thenorthern corn leaf blight highly-pathogenic strains; 3) preparation of second-level strains; 4) preparation of the inoculant; 5) culture of the inoculant. The inoculant and the preparation method thereof disclosed by the invention have the following advantages: the strains for northern corn leaf blight disease resistance identification has strong pathogenicity, high purity and high activity, and thus the accuracy and the high efficiency of corn disease resistance identification are guaranteed; the working efficiency is greatly improved compared with a plate medium; injection of gnats and hybrid bacteria is avoided, and a high-quality finished product of inoculant is obtained; the nutrition and the culture conditions of the inoculant are favorable for generating exserohilum turcicum conidia, the spore production is fast, a large quantity of spores are produced, and the spore production period is long.

Description

technical field [0001] The invention belongs to the technical field of plant pathogenic fungus research, and in particular relates to an inoculum for high-efficiency disease-resistant identification of maize leaf spot and a preparation method thereof. Background technique [0002] Northern Corn Leaf Blight (Northern Corn Leaf Blight) is an important leaf disease of corn. Maize spot disease was first discovered in Italy in 1876, and it has become one of the world-wide spread diseases. Big losses, severe land production drops by 30% to 50%, or even more. At present, the prevention and control of this disease is mainly based on planting disease-resistant varieties, scientific and reasonable variety layout, reducing primary infection sources, chemical control and other comprehensive technical measures. Using disease-resistant germplasm resources, breeding and planting disease-resistant varieties is the main effective measure to control the epidemic of maize leaf spot. Obtaini...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12N1/14C12R1/645
CPCC12N1/14C12Q1/18G01N2333/37
Inventor 蒙成黄艳花
Owner GUANGXI AGRI VOCATIONAL COLLEGE
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