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Preparation method of novel rat osteoarthritis model

An osteoarthritis and model technology is applied in the field of preparation of a new rat osteoarthritis model, and achieves the effects of good safety, strong near-infrared light absorption, and high photothermal conversion rate

Inactive Publication Date: 2018-08-28
SHANGHAI FIRST PEOPLES HOSPITAL
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In the prior art, there is no report about the preparation method of the rat osteoarthritis model of the present invention

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  • Preparation method of novel rat osteoarthritis model
  • Preparation method of novel rat osteoarthritis model
  • Preparation method of novel rat osteoarthritis model

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Embodiment 1

[0026] The preparation method of embodiment 1 novel rat osteoarthritis model

[0027] 1. Modeling method

[0028] 1. Preparation of photothermal nanomaterial Cu 9 S 5 @SiO 2 , the photothermal nanomaterial used Cu 9 S 5 @SiO 2 Prepared by Donghua University according to the preparation methods of published papers (Tian Q, Jiang F, Zou R, et al. HydrophilicCu 9 S 5 nanocrystals: a photothermal agent with a 25.7% heat conversion efficiency for photothermal ablation of cancer cells in vivo [J]. ACS Nano, 2011, (12): 9761-9771), its physical form was verified by electron microscopy.

[0029] 2. SD male rats, 6-8 weeks old, weighing 180-240 grams, were intraperitoneally anesthetized with 1% pentobarbital at a dose of 40 mg / Kg. Intra-articular injection of 100 μg / ml mixed Cu 9 S 5 @SiO 2 , injected into the rat knee joint cavity through the infrapatellar ligament of the rat.

[0030] 3. Use 980nm, 0.72W / cm 2 The knee joints of rats injected with materials were irradiate...

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Abstract

The invention discloses a preparation method of a novel rat osteoarthritis model. The preparation method comprises the following steps of (1), preparing a photothermal nano material Cu9S5@SiO2; (2), conducting intraperitoneal anesthesia on abdominal cavities of SD male rats at the age of 6-8 weeks and with the weight of 180-240 g by using 1% pentobarbital at a dose of 40 mg / Kg, injecting 100 microliters of 100 Mug / ml evenly mixed Cu9S5@SiO2 solution into articular cavities, and injecting into knee joint cavities of the rats through infrapatellar ligaments of the rats; (3), using a 980 nm, 0.72W / cm<2> near-infrared laser for irradiation of rat knee joints, wherein the irradiation continues to be conducted for 10 min after the maximum temperature of the rat knee joints exceeds 50 DEG C; (4), after 4 weeks, taking right knee joints of the SD rats to evaluate the damage situation through knee joint cartilage HE staining. The method is simple to operate, quantification and precise controlof energy damage can be achieved, and the temperature rise condition can be monitored under a thermal imager to achieve the visualization of the damage process.

Description

technical field [0001] The invention relates to a method for establishing an animal model for testing, in particular to a method for preparing a novel rat osteoarthritis model. Background technique [0002] Osteoarthritis is a common chronic movement system disease that occurs in the knee joints of the elderly and can cause joint pain and lead to disability. With the increase of the elderly population in our country, the number of patients with osteoarthritis is gradually increasing. The treatment of osteoarthritis includes conservative treatment and surgical treatment. Conservative treatment such as taking non-steroidal anti-inflammatory drugs (NSAID) can temporarily relieve pain. Surgical treatment mainly includes arthroscopic debridement, high fibular osteotomy and joint replacement. . Given the risks and costs of surgery, further research into the disease osteoarthritis and new treatments are warranted. [0003] The pathogenesis of osteoarthritis is unclear, and wear ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00
CPCA61K41/0052
Inventor 王秋根周峰邓国英张菲沙一帆
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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