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A method for improving the survival rate of camellia grafting

A technology of survival rate and camellia, which is applied in the field of improving the survival rate of camellia grafting, can solve the problems of increasing the risk of bacterial infection, low survival rate of grafted seedlings, and great difference in survival rate, so as to improve callus vitality and promote callus Tissue growth, the effect of increasing the number of lateral buds

Active Publication Date: 2019-08-16
杭州富阳优信科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent "201710737138, Grafting method for improving the survival rate of camellias" uses a mixture of indole butyric acid, naphthalene acetic acid and vitamin C to help the wound tissue of the rootstock and scion to heal as soon as possible, thereby improving the survival rate of the scion, and the grafting survival rate reaches More than 93.6%. However, the grafting methods and survival rates of different camellia varieties vary greatly. This method does not disclose the specific type. The applicant found through experiments that the above-mentioned method can achieve a survival rate of about 90%. However, for some Yunnan Camellia, the survival rate is only between 50-80%, and the varieties that are difficult to graft and survive, such as Tongzimian, have a survival rate of less than 50%. For the defects of slow wound healing of grafted plants and low survival rate of grafted seedlings, China Invention patent "2017104980905, a method of improving the survival rate of camellia grafting" uses solid agar to coat the grafting site to form an agar layer, which can promote moisturizing and nutrient absorption, but increases the risk of bacterial infection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for improving the survival rate of camellia grafting, comprising the steps of:

[0033] Configure biostimulants: Streak inoculate Fusarium chlamysporum on PDA medium for culture to obtain a single colony; pick a single colony and inoculate it on the seed medium for culture, and cultivate at 30°C for 24 hours to obtain the seed liquid of Fusarium chlamysporum. Use; the formula of described seed medium is: glucose 10g / L, yeast extract 6g / L, dipotassium hydrogen phosphate 1g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.01g / L, ferrous sulfate 0.01g / L;

[0034] Inoculate Cladosporium on PDA medium and cultivate to obtain a single colony; pick a single colony and inoculate it on PDA liquid medium for cultivation, and cultivate at 30°C for 24 hours to obtain Cladosporium seed liquid, which is ready for use;

[0035] Inoculate the seed solution of Fusarium chrysosporum and Cladosporium into MS medium in sequence, the inoculum amounts were 3% and 5% res...

Embodiment 2

[0042] A method for improving the survival rate of camellia grafting, comprising the steps of:

[0043]Configure biostimulants: Streak inoculate Fusarium chlamysporum on PDA medium for culture to obtain a single colony; pick a single colony and inoculate it on the seed medium for culture, and cultivate at 30°C for 24 hours to obtain the seed liquid of Fusarium chlamysporum. Use; the formula of described seed medium is: glucose 10g / L, yeast extract 6g / L, dipotassium hydrogen phosphate 1g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.01g / L, ferrous sulfate 0.01g / L;

[0044] Inoculate Cladosporium on PDA medium and cultivate to obtain a single colony; pick a single colony and inoculate it on PDA liquid medium for cultivation, and cultivate at 30°C for 24 hours to obtain Cladosporium seed liquid, which is ready for use;

[0045] Inoculate the seed solution of Fusarium chlamysporum and Cladosporium in sequence into MS medium, the inoculation amounts were 4% and 7%, ...

Embodiment 3

[0052] Taking Example 1 as an example, the impact of biostimulants on grafting has been detected:

[0053] A multi-factor test was used to select different groups for testing. See Table 1 for the specific classification:

[0054] Table 1

[0055] factor Group 1 (Example 1) group 2 group 3 group 4 Group 5 Fusarium chlamysporum seed solution ＋ － ＋ ＋ － Cladosporium seed solution ＋ ＋ － ＋ － 6-BA+NAA+DA-6 ＋ ＋ ＋ － ＋

[0056] According to Table 1, five groups were set up, which were divided into five test fields, and all the other treatment methods were the same as in Example 1. 10 plants were randomly selected from each test field as test objects, and the average index was calculated. The statistical results of the survival rate of scion, the growth of scion and the number of lateral buds in each group are shown in Table 2:

[0057] Table 2

[0058] group Scion survival rate% Spike growth cm The number of latera...

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Abstract

The invention belongs to the technical field of plant grafting and propagation, and discloses a method for improving the grafting survival rate of camellia. The method includes the following steps of1, preparing a biostimulation agent; 2, conducting rootstock treatment; 3, conducting scion treatment; 4, grafting; 5, conducting aftertreatment. According to the method, by adopting ordinary oil-teacamellia as rootstocks, rare yunnan black tea trees are grafted, by adopting the efficient biostimulation agent, the survival rate of the yunnan black tea trees is increased, and the growth quantity of scions and the shooting quantity of lateral buds are obviously increased.

Description

technical field [0001] The invention belongs to the technical field of plant grafting and propagation, and in particular relates to a method for improving the survival rate of camellia grafting. Background technique [0002] Grafting refers to the technology of transferring the buds and branches of an excellent plant variety to the stem and root of another plant, and making them heal to form a new independent plant. It is a technology widely used in the process of seedling cultivation and production of horticultural crops. The biological principle of graft survival is that a layer of brown septum is formed from the residue of dead cells on the surface of the cut surface of the stock and ear, and then the cells around the wound are divided due to the action of the callus, and the cambium cells are active, breaking through the septum to form a callus. Injury tissue, callus cells differentiate further, form new xylem inwardly, and form new phloem outwardly, connect the xylem ve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G2/30A01G2/35
Inventor 潘文鋆赵志华吕伟红赵成龙吕伟德
Owner 杭州富阳优信科技有限公司
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